International Journal of Agricultural Sciences

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Optimization of Explants Density for Tissue Culture Propagation of Banana cv. 'GRANDE NAINE'


Affiliations
1 Center for Horticulture Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot (Karnataka), India
2 Division of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), India
     

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Cost of production is always stressed as the main obstacle for tissue culture. Nutrient media is one of the most costly input which accounts for 30-35 per cent of total cost of tissue culture propagation. Production cost, therefore, can be reduced by efficient utilization of culture media. To find out optimum quantity of media required for shoot proliferation, 1-5 multiple bud explants were incubated in each culture bottle. Explants density of 4/culture bottles was found best as it recorded higher total shoot production/l (291.94), shoot length (3.22 cm), number of leaves/shoot (2.81) and lower cost per shoot (Rs. 1.175). In vitro ischolar_maining was carried out with densities of 6, 8, 10 and 12 microshoots/culture bottle. The maximum response with regard to ischolar_maining had not yet been reached as there were no significant differences among the treatments. Incubation of 4 multiple bud explants and 12 microshoots per culture bottles is optimum for scaling-up of tissue culture production of banana cv. 'GRANDE NAINE'.

Keywords

Tissue Culture, Explants Density, Grande Naine, MS Medium, Cost/Shoot.
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  • Arinaitwe, G., Rubaihayo, P.R. and Magamboo, M.J.S. (2000). Proliferation rate effects of cytokinins on banana (Musa sp.) cultivars. Sci. Hort., 86: 13-21.

  • Besagarahally, R. (1996). Micro propagation and nutritional studies in of tissue cultured banana var. GRANDE NAINE. Ph. D. Thesis, University of Agricultural Sciences, Bangalore, KARNATAKA (INDIA). pp. 38-39.

  • Chun, Y.W., Hall, R.B. and Stephens, L.C. (1986). Influences of medium consistency and shoot density on in vitro shoot proliferation of Populus alba × P. grandidentata. Plnat Cell Tiss. Org. Cult., 5 : 179-185.

  • Goel, M.K., Kukreja, A.K. and Khanuja, S.P.S. (2007).Cost effective approaches for in vitro Mass propagation of Rauwolfia serpentina benth. Ex Kurz. Asian J. Plant Sci., 6: 957-961.

  • Hamad, A.M. and Taha, R.M. (2009). Effect of explants density on in vitro proliferation and growth of separated and cluster shoots of smooth cayenne pineapple [Ananas comosus (L.) Merr.]. Asian J. Plant Sci., 8 (4) : 313-317.

  • Matsubara, S. (1973). Population effect in lateral bud culture of asparagus and promotion of ischolar_main formation by transplanting. J. Jnp. Hort. Sci., 42: 142–146.

  • Matsubara, S. and Clore, W.J. (1974).Vegetative propagation of asparagus from lateral buds. Sci. Rep. Fac. Agric. Okayama Univ., 43: 19–26.

  • McClelland, M.T. and Smith, M.A.L. (1990). Vessel type, closure and explant orientation influence in vitro performance of five woody species. Hort. Sci., 25 (7): 797–800.

  • Monette, P.L. (1983). Influence of size of culture vessel on in vitro proliferation of grape in liquid medium. Plant Cell Tiss. Org. Cult., 2: 327–332.

  • Monette P.L. (1986). Cold storage of kiwi shoot tips in vitro. Hort. Sci., 21: 1203-1205.

  • Sarkar, D., Chandra, R. and Naik, P.S. (1997). Effect of inoculation density on potato Micro propagation. Plant Cell Tiss. Org. Cult., 48 : 63-66.

  • Start, N.E. and Cumming, B.G. (1976). In vitro propagation of Saintpaulia ionantha Wendl. Hort.Sci., 11: 204–206.

  • Anonymous (2002). http://faostat.fao.org/faostat/collections?subset = agriculture.


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  • Optimization of Explants Density for Tissue Culture Propagation of Banana cv. 'GRANDE NAINE'

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Authors

G. Prabhuling
Center for Horticulture Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot (Karnataka), India
B. N. Sathyanarayana
Division of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), India

Abstract


Cost of production is always stressed as the main obstacle for tissue culture. Nutrient media is one of the most costly input which accounts for 30-35 per cent of total cost of tissue culture propagation. Production cost, therefore, can be reduced by efficient utilization of culture media. To find out optimum quantity of media required for shoot proliferation, 1-5 multiple bud explants were incubated in each culture bottle. Explants density of 4/culture bottles was found best as it recorded higher total shoot production/l (291.94), shoot length (3.22 cm), number of leaves/shoot (2.81) and lower cost per shoot (Rs. 1.175). In vitro ischolar_maining was carried out with densities of 6, 8, 10 and 12 microshoots/culture bottle. The maximum response with regard to ischolar_maining had not yet been reached as there were no significant differences among the treatments. Incubation of 4 multiple bud explants and 12 microshoots per culture bottles is optimum for scaling-up of tissue culture production of banana cv. 'GRANDE NAINE'.

Keywords


Tissue Culture, Explants Density, Grande Naine, MS Medium, Cost/Shoot.

References