International Journal of Agricultural Sciences

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Cheaper Carbon Sources for Micropropagation of Banana cv. 'GRANDE NAINE'


Affiliations
1 Center for Horticulture Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot (Karnataka), India
2 Division of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), India
     

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Micropropagation of banana has become a routine procedure but high production cost is limiting the commercial use of tissue culture technology. Analytical grade sucrose is the most commonly used carbon source for the micropropagation banana, however, the cost is too high to justify the use at commercial scale. Therefore, inexpensive and readily available sources of carbon such as laboratory grade sucrose, common grade sugar, cube sugar, rock sugar, candy sugar, glucose, jaggery and sugarcane juice were evaluated for in vitro propagation of banana cv. 'GRANDE NAINE'. Best response in terms of shoot multiplication and ischolar_maining were achieved with rock sugar and common grade sugar, respectively which could be compared well with that of analytical grade sucrose. The results showed the possibility of successful use of cheaper carbon sources for micropropagation of banana cv. 'GRANDE NAINE'.

Keywords

Analytical Grade Sucrose, Common Grade Sugar, Grande Naine, Micropropagation, Rock Sugar.
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  • Cheaper Carbon Sources for Micropropagation of Banana cv. 'GRANDE NAINE'

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Authors

G. Prabhuling
Center for Horticulture Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot (Karnataka), India
B. N. Sathyanarayana
Division of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), India

Abstract


Micropropagation of banana has become a routine procedure but high production cost is limiting the commercial use of tissue culture technology. Analytical grade sucrose is the most commonly used carbon source for the micropropagation banana, however, the cost is too high to justify the use at commercial scale. Therefore, inexpensive and readily available sources of carbon such as laboratory grade sucrose, common grade sugar, cube sugar, rock sugar, candy sugar, glucose, jaggery and sugarcane juice were evaluated for in vitro propagation of banana cv. 'GRANDE NAINE'. Best response in terms of shoot multiplication and ischolar_maining were achieved with rock sugar and common grade sugar, respectively which could be compared well with that of analytical grade sucrose. The results showed the possibility of successful use of cheaper carbon sources for micropropagation of banana cv. 'GRANDE NAINE'.

Keywords


Analytical Grade Sucrose, Common Grade Sugar, Grande Naine, Micropropagation, Rock Sugar.

References