International Journal of Agricultural Sciences

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Cheaper Carbon Sources for Micropropagation of Banana cv. 'GRANDE NAINE'


Affiliations
1 Center for Horticulture Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot (Karnataka), India
2 Division of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), India
     

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Micropropagation of banana has become a routine procedure but high production cost is limiting the commercial use of tissue culture technology. Analytical grade sucrose is the most commonly used carbon source for the micropropagation banana, however, the cost is too high to justify the use at commercial scale. Therefore, inexpensive and readily available sources of carbon such as laboratory grade sucrose, common grade sugar, cube sugar, rock sugar, candy sugar, glucose, jaggery and sugarcane juice were evaluated for in vitro propagation of banana cv. 'GRANDE NAINE'. Best response in terms of shoot multiplication and ischolar_maining were achieved with rock sugar and common grade sugar, respectively which could be compared well with that of analytical grade sucrose. The results showed the possibility of successful use of cheaper carbon sources for micropropagation of banana cv. 'GRANDE NAINE'.

Keywords

Analytical Grade Sucrose, Common Grade Sugar, Grande Naine, Micropropagation, Rock Sugar.
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  • Das, A. and Gupta, S.N. (2009). Use of low cost resources for banana micropropagation. Indian J. Hort., 66 (3): 295-300.

  • Debnath, S.C. (2005). Effect of carbon sources and concentration on development of Lingonberry (Vaccinium vitis-idea L.) shoot cultivated in vitro from nodal explants. In-Vitro Cell Dev. Biol. Plant., 41: 145-150.

  • Demo, P., Kuria, P., Nyende, A.B. and Kahangi, E.M. (2008). Table sugar as an alternative low cost medium component for in vitro micropropagation of potato (Solanum tuberosum L.). African J. Biotech., 7 (15) : 2578-2584.

  • Ganapathi, T.R., Moha, J.S.S., Supranna, P., Bapat, V.A. and Rao, P.S. (1995). A low cost strategy for in vitro propagation of banana. Curr. Sci., 68 : 646-650.

  • George, E.F. (1993). Plant propagation by tissue culture. Part 1- The technology. Exogetics Limitted.pp.377-356.

  • Goel, M.K., Kukreja, A.K. and Khanuja, S.P.S. (2007). Cost effective approaches for in vitro mass propagation of Rauwolfia serpentine Benth.Ex Kurz. Asian J. Plant Sci., 6 (6): 957-961.

  • Joshi, P., Tridevi, R. and Purohit, S.D. (2009). Micropropagation of Wrightia tomentosa: Effect of gelling agents, carbon sources and vessel type. Indian J. Biotech., 8: 115-120.

  • Kaur, R., Gautam, H. and Sharma, D.R. (2005). A low cost strategy for micropropagation of Strawberry (Fragaria X ananassa Duch.). Acta Hort., 696 : 129-133.

  • Kodym, A. and Zapata-Arias, F.J. (2001). Low- cost alternative for the micropropagation of banana. Plant Cell Tiss. Org. Cult., 66 : 67-71.

  • Prabhakara, H.L. (1999). Studies on in vitro propagation of Anthurium andreanum Lind. M. Sc. (Ag.) Thesis, University of Agricultural Sciences, Dharwad, KARNATAKA (INDIA).

  • Prakash, S. (1993). Production of ginger and turmeric through tissue culture methods and investigations into making tissue culture propagation less expensive. Ph. D. Thesis, Bangalore University, Bangalore, KARNATAKA (INDIA).

  • Prakash, S., Hoque, M.I. and Brinks, T. (2004). Culture media and containers. In: Low cost options for tissue culture technology in developing countries, proceedings of a technical meeting organized by the joint FAO/IAEA division of nuclear techniques in food and agriculture held at Vienna, 26 30 August 2002, pp. 29-40.

  • Perata, P., Matsukura, C., Vernieri, P. and Yamaguchi, J. (1997). Sugar repression of a gibberellin dependent signaling pathway in barley embryos. The Plant Cell., 9 : 2197-2208.

  • Raghu, A.V., Martin, G., Priya, V., Geetha, S.P. and Balachandran, I. (2007). Low cost alternatives for the micropropagation of Centella asiatica. J. Plant Sci., 2(6): 592-599.

  • Robert-Oehlschlager, S. L. (1988). In vitro embryogenesis in cultured barley pollen. Ph. D. Thesis, University of East Anglia.

  • Saeed, B.A. (2006). In vitro selection and evaluation of the genetic purity of regenerated banana plants. Ph. D. Thesis, University of Karachi, Karachi (Pakistan).

  • Sharma, T.R. and Singh, B.M. (1995). Simple and cost-effective medium for propagation of ginger (Zingiber officinale). Indian J. Agric. Sci., 65 (7) : 506-508.

  • Thompson, M. R. and Thorpe, T. A. (1987). Metabolic and non-metabolic roles of carbohydrates. In: Bonga, J. M.; Durzan, D. J., eds. Cell and tissue culture in forestry, Vol. 1. General principles and biotechnogy. Dordrecht: Martinus Nijnoff Publishers, pp. 89-112.

  • Zapata, A. (2001). Cost reduction in tissue culture of banana (Special Leaflet). Int. Atom Energy Labs. Agri. Biotech. Lab., Austria.


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  • Cheaper Carbon Sources for Micropropagation of Banana cv. 'GRANDE NAINE'

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Authors

G. Prabhuling
Center for Horticulture Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot (Karnataka), India
B. N. Sathyanarayana
Division of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), India

Abstract


Micropropagation of banana has become a routine procedure but high production cost is limiting the commercial use of tissue culture technology. Analytical grade sucrose is the most commonly used carbon source for the micropropagation banana, however, the cost is too high to justify the use at commercial scale. Therefore, inexpensive and readily available sources of carbon such as laboratory grade sucrose, common grade sugar, cube sugar, rock sugar, candy sugar, glucose, jaggery and sugarcane juice were evaluated for in vitro propagation of banana cv. 'GRANDE NAINE'. Best response in terms of shoot multiplication and ischolar_maining were achieved with rock sugar and common grade sugar, respectively which could be compared well with that of analytical grade sucrose. The results showed the possibility of successful use of cheaper carbon sources for micropropagation of banana cv. 'GRANDE NAINE'.

Keywords


Analytical Grade Sucrose, Common Grade Sugar, Grande Naine, Micropropagation, Rock Sugar.

References