International Journal of Agricultural Sciences

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AFLP Analysis of Genetic Relationships and Diversity of Some Oriental Pickling Melon (Cucumis melo Var. conomon) Genotypes in Karnataka


Affiliations
1 Department of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), India
2 Directorate of Education, University of Horticultural Sciences, Udyanagiri, Bagalkot (Karnataka), India
3 Department of Genetics and Plant Breeding, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), India
     

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Genetic diversity of indigenous plant population is the prerequisite for any research on plant breeding and improvement, making genetic diversity conservation of critical importance. The present study was conducted to assess the amplified fragment length polymorphism (AFLP) marker based diversity in 15 oriental pickling melon (Cucumis melo var. conomon) genotypes during 2009 at University of Agricultural Sciences (UAS), Bangalore. Thirteen primer combinations generated a total of 443 amplicons, of which 342 were polymorphic (76%) with an average of 26.31 bands. The primer combination E-CTT/M-GTG was found to amplify 45 scorable bands, with 91.11 per cent polymorphic bands. Cluster analysis based on Jaccard similarity index and UPGMA algorithm showed a high variation within the studied genotypes and a mean similarity index of 0.79 for AFLP markers. The genotypes CMC GKVK 1 and CMC GKVK 6 expressed the least similarity (0.627) and may serves as very good source in melon breeding programme. Genotypes CMC GKVK1, CMC GKVK8, CMC GKVK15, CMC GKVK11 and CMC GKVK6 fell into solitary clusters and these accessions could be used in future genetic, physiological and morphological studies.

Keywords

Oriental Pickling Melon, AFLP, Genetic Diversity.
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  • Behera, T.K., Gaikward, A.B., Singh, A.K. and Staub, J.E. (2008). Relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia L.). J. Sci. Food Agric., 88 :733-737.

  • Benbouza, H., Jacquemin, J.M., Baudoin, J.P. and Mergeai, G. (2006). Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels, Biotechnol. Agron. Soc. Environ, 10: 77-81.

  • Doyle, J.J. and Doyle, J.L. (1987). A rapid DNA isolation procedure for sample quantities of fresh leaf tissue. Phytochem. Bul., 191:11-15.

  • Garcia, M.J., Oliver, M.H., Gomez, P.H. and Devicente, M.C. (2000). Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon. Theor. Appl. Genet., 101: 860-864.

  • Given, D.R., Bran,Well D., Haman, O., Heywood, V. and Synge, H. (1987). What the conservationist requires of ex situ collections. Botanic Gardens and the World Conservation Strategy. Academic Press, London , 103-116.

  • Jaccard, P. (1908). Nouvelles recherches sur la distribution florale, Bull. Soc. Vaud. Sci. Nat., 44 : 223-270.

  • Lombard, V., Baril, C.P. , Dubreuil, P. F. Blouet and Zhang, D. (2000). Genetic relationship and finger printing of rapeseed cultivars by AFLP: Consequences for varietal registration. Crop Sci. 40:1417-1425.

  • Luan, F., Sheng, Y., Wang, Y. and Staub, J.E. (2010). Performance of melon hybrids derived from parents of diverse geographic origins. Euphytica., 173 :1-16.

  • Maras, M.J., Sustar-Vozlic, B. Javornik and Meglic, V. (2008). The efficiency of AFLP and SSR markers in genetic diversity estimation and gene pool classification of common bean (Phaseolus vulgaris L.). Acta Agric. Slovenica., 91:87-96.

  • Padmavathi, N., Tomason, Y. R., Jeong, J., Gopinath, V., Levi, A., Gibson, P. and Reddy, U.K. (2008). Molecular diversity in the Ukrainian melon collection as revealed by AFLPs and microsatellites. Plant Genetic Resources: Characterizat. & Utilizat., 7(2): 127-134.

  • Park, Y.H., Kim, K.H., Choi, Y.M., Choi, H.S., Chae, Y., Park, K.S. and Chung, S.M. (2010). Evaluation of TYLCV- resistant tomato germplasm using molecular markers. Kor. J. Hort. Sci. Technol., 28: 89-97.

  • Raghami, M., Zamani, Z., Lopez-Sese, A.I., Hasandokht, M.R., Moghadam Fattahi, M.R. and Kashi, A. (2014). Genetic diversity among melon accessions from Iran and their relationships with melon germplasm of diverse origins using microsatellite markers. Plant Syst. Evol., 300:139-151.

  • Ramanatha, R.V. and Hodgkin, T. (2002). Genetic diversity and conservation and utilization of plant genetic resources. Plant Cell Tissue & Organ Cult., 68:1-19.

  • Reddy, O., Tomason, Y.R., Zlenko, I., Bashet, A., Kaur, P., Levi, A., Bates, G.T. and Nimmakayala, P. (2005). Molecular diversity in ukrainian melon germplasm. Congr. Plant Animal Genomes. Proceed. Plant Animal Genomes, 13: 177.

  • Rohlf, F.J. (2000). NTSYS-pc: Numerical taxonomy and multivariate analysis system, version 2.1, Exeter Publishing, Setauket, NEW YORK, U.S.A.

  • Sekhon, M.S. and Gupta, V.P. (1995). Genetic distance and heterosis in Indian mustard: developmental isozymes as indicator of genetic relationships. Theo. Appl. Genet., 91:1148-1154.

  • Sheng, Y.Y., Wang, Y.H. and Luan, F.S. (2011).Distribution of simple sequence repeat and AFLP marker on melon genetic map1. China Veget., 1 (8) : 39-45.

  • Vos, P., Hogers, R., Bleeker, M., Reijang, M., Van De Lee, T., Hornes, M., Frijters, A., Plot, J., Kuiper, M. and Zabeau, M. (1995). AFLP a new technique for DNA finger printing. Nucleic Acids Res., 23 : 4407-4444.

  • Xu, Z., Hu, T. and Zhang, F. (2012). Genetic diversity of walnut revealed by AFLP and RAPD markers. J. Agric. Sci., 4 : 271-276.

  • Yashiro, K., Hirouoshi, I., Yukari, A. and Ken,O.T. (2005). Genetic relationships among Asian melon (Cucumis melo L.) Revealed by AFLP analysis. Breed. Sci., 55:197-206.


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  • AFLP Analysis of Genetic Relationships and Diversity of Some Oriental Pickling Melon (Cucumis melo Var. conomon) Genotypes in Karnataka

Abstract Views: 383  |  PDF Views: 0

Authors

L. Mukunda Lakshmi
Department of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), India
H. B. Lingaiah
Directorate of Education, University of Horticultural Sciences, Udyanagiri, Bagalkot (Karnataka), India
A. Mohan Rao
Department of Genetics and Plant Breeding, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), India
S. Ramesh
Department of Genetics and Plant Breeding, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), India

Abstract


Genetic diversity of indigenous plant population is the prerequisite for any research on plant breeding and improvement, making genetic diversity conservation of critical importance. The present study was conducted to assess the amplified fragment length polymorphism (AFLP) marker based diversity in 15 oriental pickling melon (Cucumis melo var. conomon) genotypes during 2009 at University of Agricultural Sciences (UAS), Bangalore. Thirteen primer combinations generated a total of 443 amplicons, of which 342 were polymorphic (76%) with an average of 26.31 bands. The primer combination E-CTT/M-GTG was found to amplify 45 scorable bands, with 91.11 per cent polymorphic bands. Cluster analysis based on Jaccard similarity index and UPGMA algorithm showed a high variation within the studied genotypes and a mean similarity index of 0.79 for AFLP markers. The genotypes CMC GKVK 1 and CMC GKVK 6 expressed the least similarity (0.627) and may serves as very good source in melon breeding programme. Genotypes CMC GKVK1, CMC GKVK8, CMC GKVK15, CMC GKVK11 and CMC GKVK6 fell into solitary clusters and these accessions could be used in future genetic, physiological and morphological studies.

Keywords


Oriental Pickling Melon, AFLP, Genetic Diversity.

References