International Journal of Agricultural Sciences

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Free Radical Quenching Effect of Botanical Treatments on Storability of Greengram Seeds


Affiliations
1 Agricultural College and Research Institute (T.N.A.U.), Thanjavur (T.N.), India
     

   Subscribe/Renew Journal


Storage of seeds enhances the longevity of the seed for sowing in the following season. Seed quality is the prerequisite condition that affects the germination and yield of any crop plants. The studies were carried out to determine the effects of three botanicals on greengram seeds storage. Laboratory studies were conducted with leaf powders of three plants to show the preservative effect for maintaining the quality of greengram seeds in storage. After processing and drying, seeds were preserved with different botanicals such as Albizia amara, Azadirachta indica, Phyllanthus emblica at a dose of 10g/kg and halogen mixture @3g/kg stored in gada cloth bags. At the end of six and twelve months, the germination percentage and the changes in the antioxidant enzymes such as superoxide dismutase, catalase, polyphenoloxidase and peroxidase were determined in the treated seeds. The results revealed that, the botanicals and halogen mixture treatments were significantly effective in controlling and maintaining higher seed quality throughout the storage period when compared to untreated control. Among the botanicals, the Albizia amara was found better by recording significantly higher germination percentage and enzymatic levels were retained when compared to untreated control seeds during storage. From this study, it is revealed that uses of different botanicals are less costly, easily available to the farmers and safe to handle.

Keywords

Greengram, Botanicals, Storage, Seed Quality, Biochemical Analysis.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Atici,O., Agar,G. and Battal, P. (2007). Influence of long term storage on plant growth substance levels, germination and seedling growth in legume seeds stored for 37 years. Indian J.Plant Physiol., 12: 1–5.

  • Bailly, C. (2004). Reactive oxygen species and antioxidants in seed biology. Seed Sci. Res.,14 : 93–107.

  • Chaiyasit, W., Elias, R..J., McClements, D.J. and Decker, E.A. (2007). Role of physical structures in bulk oils on lipid oxidation. Criti. Rev. Food Sci. &Nutr., 47: 299–317.

  • Copeland, L.O. and Mcdonald, Jr. M.B.(1995). Principles of seed science and technology. New York: McMillan, 321pp.

  • De, Meyer G., Capieau, K., Audenaert, K., Buchala, A., Metraux, J.P. and Hotfi te, M. (1999).Nanogram amounts of salicyclic acid produced by the rhizobacterium Pseudomonas aeruginosa 7NSK 2 activate the systemic acquired resistance pathway in bean. Molecular Plant-Microbe Interaction, 12 : 450–458.

  • Esterbauer, H., Schwarzl, E. and Hayn, M. (1977). A rapid assay for catechol oxidase and laccase using 2, nitro-5thiobenzoic acid, Anal. Biochem., 77 : 486-494.

  • Ferguson, J.M., TeKrony, D.M. and Egli, D.B. (1990). Changes during early soybean seed and axes deterioration: II. Lipids. Crop Sci., 30: 179-182.

  • Goel, A., Goel,A.K. and Sheoran, I.S. (2003). Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. J. Plant Physiol.,160: 1093– 100.

  • Hsu, C.C., Chen, C. L., Chen, J. J. and Sung, J. M. (2003). Accelerated ageing enhanced lipid peroxidation in bitter gourd seeds and effects of priming and hot water soaking treatments. Sci. Hort., 98: 201-212.

  • Kakkar, P., Das, B. and Viswanathan, P.N. (1984). A modified spectrophotometric assay of superoxide dismutase, Indian J. Biochem. Biophys., 21 : 130-132.

  • Laloi, C., Ape, K. and Danon, A. (2004). Reactive oxygen signalling: the latest news. Curr. Opinion Plant Biol., 7: 323328.

  • Lapcik, O. Hampl, R., Hill, M. and Starka,(1999). Immunoassay of 7-hydroxysteroids: 2. Radioimmunoassay of 7ahydroxy-dehydroepiandrosterone. J. Steroid Biochem. Mol. Biol., 71: 231-237.

  • Lehner, A., Mamadou,N., Poels, P., Côme, D., Bailly, C. and Corbineau, F. (2008). Changes in soluble carbohydrates, lipid peroxidation and antioxidant enzyme activities in the embryo during ageing in wheat grains. J. Cereal Sci.,47: 555-565.

  • Loycrajjou, Lovigny Y., Steven, P.C., Gischolar_main, Belghazi, M., Job, C. and Job, D. (2008). Proteome wide characterization of seed ageing in arabidopsis. A comparison between artificial and natural ageing. Prot. Pl. Phy., 148 : 620–641.

  • Luck, H. (1974). In: Methods in enzymatic analysis, II Edn., Bergmeyer, New York, pp.805.

  • McDonald, M. B. (1999). Seed deterioration: Physiology, repair and assessment. Seed Sci. and Technol., 27 (1):177-237.

  • McDonald, M.B. (2004). Orthodox seed deterioration and its repair, pp. 273-304. In: Handbook of seed physiology: Applications to agriculture, Benech-Arnold, R. L. and R.A. Sanchez (Eds.). Food Products Press, New York, U.S.A.

  • Merritt, D.J., Senaratna, T., Touchell, D.H., Dixon, K.W. and Sivasithamparam, K., (2003). Seed ageing of four Western Australian species in relation to storage environment and seed antioxidant activity. Seed Sci. Res.,13: 155–65.

  • Murthy, U. M. N., Kumar, P. P. and Sun, W. Q. (2003). Mechanisms of seed ageing under different storage conditions for Vigna radiata (L.) Wilczek: Lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition. J. Exp. Bot., 54 :1057-1067.

  • Pallavi, M., Sudheer, S.K., Dangi, K.S. and Reddy, A.V. (2003). Effect of seed ageing on physiological, biochemical and yield attributes in sunflower (Helianthus annus L.) cv. MORDEN. Seed Research, 31(2): 161–168.

  • Pal, P. and Basu, R.N. (1994). Extension of wheat seed viability with plant leaf powder and an aspirin containing pharmaceutical preparation. Indian Agriculturist, 38: 145-149.

  • Pal, P. and Basu, R.N. (1988). Treatment of rice seed with chlorine for the maintenance of vigour, viability and productivity. Indian Agric., 32 : 71- 75.

  • Popovic, B. (2006). Uticaj γ- zracenja na antioksidantni sistem odabranih genotipova soje i pojava oksidativnog stresa. Doktorska disertacija, Prirodno-matematièki fakultet, Departman za hemiju, Novi Sad. pp.35-37.

  • Pukacka, S. and Ratajczak, E. (2007). Age-related biochemical changes during storage of beech (Fagus sylvatica L.) seeds. Seed Sci. Res., 17: 45–53.

  • Reddy, K.P., Subhani, S.M., Khan, P.A. and Kumar, K.B. (1995). Effect of light and benzyladenine on darktreated growing rice (Oryza sativa) leaves-changes in peroxidase activity, Plant Cell Physiol., 26 : 987-994.

  • Roberts, E.H. (1972). Cytological, genetical and metabolic changes associated with loss of viability.In: Viability of seeds ed. Roberts, E.H. pp., 253- 306., London, United Kingdom.

  • Scialabba, A., Bellani, L.M. and Dell’aquila, A. (2002).Effects of ageing on peroxidase activity and localization in radish (Raphanus sativus L.) seeds. European J.Histochem., 46 (4): 351–358.

  • Shen, T.Y. and Oden, P.C. (1999).Activity of sucrose synthase, soluble acid invertase and fumarase in germinating seeds of scots pine (Pinus sylvestris L.) of different quality. Seed Sci. & Technol., 27 (3) : 825-838.


Abstract Views: 373

PDF Views: 0




  • Free Radical Quenching Effect of Botanical Treatments on Storability of Greengram Seeds

Abstract Views: 373  |  PDF Views: 0

Authors

M. Chitra
Agricultural College and Research Institute (T.N.A.U.), Thanjavur (T.N.), India
M. Pandiyan
Agricultural College and Research Institute (T.N.A.U.), Thanjavur (T.N.), India

Abstract


Storage of seeds enhances the longevity of the seed for sowing in the following season. Seed quality is the prerequisite condition that affects the germination and yield of any crop plants. The studies were carried out to determine the effects of three botanicals on greengram seeds storage. Laboratory studies were conducted with leaf powders of three plants to show the preservative effect for maintaining the quality of greengram seeds in storage. After processing and drying, seeds were preserved with different botanicals such as Albizia amara, Azadirachta indica, Phyllanthus emblica at a dose of 10g/kg and halogen mixture @3g/kg stored in gada cloth bags. At the end of six and twelve months, the germination percentage and the changes in the antioxidant enzymes such as superoxide dismutase, catalase, polyphenoloxidase and peroxidase were determined in the treated seeds. The results revealed that, the botanicals and halogen mixture treatments were significantly effective in controlling and maintaining higher seed quality throughout the storage period when compared to untreated control. Among the botanicals, the Albizia amara was found better by recording significantly higher germination percentage and enzymatic levels were retained when compared to untreated control seeds during storage. From this study, it is revealed that uses of different botanicals are less costly, easily available to the farmers and safe to handle.

Keywords


Greengram, Botanicals, Storage, Seed Quality, Biochemical Analysis.

References