International Journal of Agricultural Sciences

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Optimising Protocol for Direct Differentiation of Shoot Buds From Leaf ex-plant of Tagetes erecta L. Var. Pusa Narangi Gainda


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1 Division of Floriculture and Landscaping ICAR- Indian Agricultural Research Institute, New Delhi, India
     

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Marigold is an important loose flower crop has gained popularity for its pharmaceutical, industrial, medicinal and therapeutic importance. In this present study, we developed a protocol for direct regeneration using in-vitro raised immature leaves of African marigold (Tagetes erecta L.var. “Pusa Narangi Gainda”). A total of eight treatment combinations of growth hormones such as BAP, IAA and GA3with MS as a basal media was studied. The best medium found for direct, shoot organogenesis from leaf ex-plant was treatment (T3) - MS + BAP 0.5 mg/l + IAA 0.25 mg/l. Pre-treatment with Carbendazim (0.2 %) + Mancozeb (0.2 %) + 8-HQC (200 mg/l) for 2hr followed by HgCl2 for 4 min. resulted in minimum fungal (24.33%), bacterial (8.33%) contamination simultaneously it increased the survival percentage upto (67.33%). This study is helpful for rapid clonal propagation, production of lutein-rich pharmaceutical compounds and secondary metabolites using tissue culture techniques.

Keywords

African Marigold, Shoot Organogenesis, Leaf Ex-plant, Growth Hormones, Clonal Propagation.
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  • Optimising Protocol for Direct Differentiation of Shoot Buds From Leaf ex-plant of Tagetes erecta L. Var. Pusa Narangi Gainda

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Authors

Soudamani Karjee
Division of Floriculture and Landscaping ICAR- Indian Agricultural Research Institute, New Delhi, India
Namita
Division of Floriculture and Landscaping ICAR- Indian Agricultural Research Institute, New Delhi, India
Kanwar P. Singh
Division of Floriculture and Landscaping ICAR- Indian Agricultural Research Institute, New Delhi, India
Sapna Panwar
Division of Floriculture and Landscaping ICAR- Indian Agricultural Research Institute, New Delhi, India

Abstract


Marigold is an important loose flower crop has gained popularity for its pharmaceutical, industrial, medicinal and therapeutic importance. In this present study, we developed a protocol for direct regeneration using in-vitro raised immature leaves of African marigold (Tagetes erecta L.var. “Pusa Narangi Gainda”). A total of eight treatment combinations of growth hormones such as BAP, IAA and GA3with MS as a basal media was studied. The best medium found for direct, shoot organogenesis from leaf ex-plant was treatment (T3) - MS + BAP 0.5 mg/l + IAA 0.25 mg/l. Pre-treatment with Carbendazim (0.2 %) + Mancozeb (0.2 %) + 8-HQC (200 mg/l) for 2hr followed by HgCl2 for 4 min. resulted in minimum fungal (24.33%), bacterial (8.33%) contamination simultaneously it increased the survival percentage upto (67.33%). This study is helpful for rapid clonal propagation, production of lutein-rich pharmaceutical compounds and secondary metabolites using tissue culture techniques.

Keywords


African Marigold, Shoot Organogenesis, Leaf Ex-plant, Growth Hormones, Clonal Propagation.

References