International Journal of Medical Sciences

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Molecular Characterization of Coagulase Negative Nosocomial Staphylococcus sp. by RFLP Method


Affiliations
1 Department of Microbiology, Marudupandiyar College, Thanjavur (T.N.), India
2 Department of Environmental and Herbal Sciences, Tamil University, Thanjavur (T.N.), India
     

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Coagulase negative nosocomial Staphylococcus spp. were collected from infected patient in and around Thanjavur. Colonies were analyzed by using morphological (staining, colony morphology and pigmentation) and biochemical (catalase, coagulase, acid production etc.) analyses. Twenty coagulase negative Staphylococcus spp. (CoNS) were identified by morphological and biochemical tests and submitted to molecular typing by plasmid DNA analysis, genomic DNA analysis, restriction endonuclease digestion pattern by using EcoR I, Hind III, and Alu I enzymes. 20 species of three different type of CoNS were identified, viz., S. epidermidis, S. saprophyticus and S. haemolyticus.

Keywords

Coagulase Negative Staphylococcus spp., Genomic DNA, Plasmid DNA.
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  • Molecular Characterization of Coagulase Negative Nosocomial Staphylococcus sp. by RFLP Method

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Authors

A. Sathya
Department of Microbiology, Marudupandiyar College, Thanjavur (T.N.), India
A. Rajendran
Department of Environmental and Herbal Sciences, Tamil University, Thanjavur (T.N.), India
V. Sivakumari
Department of Environmental and Herbal Sciences, Tamil University, Thanjavur (T.N.), India

Abstract


Coagulase negative nosocomial Staphylococcus spp. were collected from infected patient in and around Thanjavur. Colonies were analyzed by using morphological (staining, colony morphology and pigmentation) and biochemical (catalase, coagulase, acid production etc.) analyses. Twenty coagulase negative Staphylococcus spp. (CoNS) were identified by morphological and biochemical tests and submitted to molecular typing by plasmid DNA analysis, genomic DNA analysis, restriction endonuclease digestion pattern by using EcoR I, Hind III, and Alu I enzymes. 20 species of three different type of CoNS were identified, viz., S. epidermidis, S. saprophyticus and S. haemolyticus.

Keywords


Coagulase Negative Staphylococcus spp., Genomic DNA, Plasmid DNA.