Polymerase Chain Reaction for Extra Pulmonary Tuberculosis in Cerebrospinal Fluid of HIV-Infected Patients

In the present study, total of 195 symptomatic patients (165 male and 30 female) were taken and positive Mycobacterium tuberculosis were seen in 82 cases. Moreover, 16 out of 82 patients were HIV positive with Mycobacterium tuberculosis. The sputum, blood, biological fluids, stools and urine samples were collected from each patient and were processed under aseptic condition. Standard tests such as acid-fast staining (AFS), culture (Lowenstein-Jensen) and Polymerase Chain Reaction (PCR) tests were carried out for Mycobacterium tuberculosis detection.