International Journal of Medical Sciences

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Anti-Cancer Activity of Arka (Calotropis procera) on HCT-15 Cancer Cell Line


Affiliations
1 School of Biotechnology, University of Jammu, Jammu (J&K), India
2 Division of Biochemistry and Plant Physiology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, FOA, Main Campus, Chatha, Jammu (J&K), India
     

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In vitro assay for cytotoxic activity of the stem-leaves of Calotropis procera was carried out against human cancer cell lines at the concentration of 10, 30 and 100μg/ml. Results revealed that the extracts of the plant possessed in vitro anticancer potential against HCT-15 (colon) cancer cell line at different concentrations. Further, the fractionation of the extracts was carried out and the fractions were tested on the same human cancer cell line. It was found that all the fractions inhibited the growth of HCT-15 at 100μg/ml except water-soluble fractions, but the significant growth inhibition was shown by the chloroform-soluble fractions of the ethanolic extract and 50% ethanolic extract.

Keywords

Calotropis procera, In vitro Cytotoxic, Human Cancer Cell Lines.
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  • Anti-Cancer Activity of Arka (Calotropis procera) on HCT-15 Cancer Cell Line

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Authors

Madhulika Bhagat
School of Biotechnology, University of Jammu, Jammu (J&K), India
Vikas Sharma
Division of Biochemistry and Plant Physiology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, FOA, Main Campus, Chatha, Jammu (J&K), India

Abstract


In vitro assay for cytotoxic activity of the stem-leaves of Calotropis procera was carried out against human cancer cell lines at the concentration of 10, 30 and 100μg/ml. Results revealed that the extracts of the plant possessed in vitro anticancer potential against HCT-15 (colon) cancer cell line at different concentrations. Further, the fractionation of the extracts was carried out and the fractions were tested on the same human cancer cell line. It was found that all the fractions inhibited the growth of HCT-15 at 100μg/ml except water-soluble fractions, but the significant growth inhibition was shown by the chloroform-soluble fractions of the ethanolic extract and 50% ethanolic extract.

Keywords


Calotropis procera, In vitro Cytotoxic, Human Cancer Cell Lines.