International Journal of Plant Protection

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Assessment of Temperature Stability of Nanoparticle Formulated Vinblastine


Affiliations
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
     

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Catharanthus roseus L. (Madagascar periwinkle) is regarded as a rich source of pharmaceutically important terpenoid indole alkaloids(TIAs) which are valued highly due to their wide spectrum of pharmaceutical application. Among these, vinblastine and vincristine are of particular importance because of their wide use in cancer chemotherapy. Vinblastine can have extensive use for the treatment of lung cancer, breast cancer, head and neck cancer and testicular cancer. It is also used to treat Langerhens cell histocytosis. This study was done to assess the stability of vinblastine. The extracted crude vinblastine was formulated with nanoparticles at different temperature. Stability was determined at varying temperature. Reduction method was used to synthesize silver nanoparticles, LDH was synthesized using co-precipitation method and MMT was purchased from Sigma-Aldrich.LDH and MMT formulated vinblastine showed maximum temperature stability at 60oC and 80oC. The increase in heat stability will help in increasing the shelf-life as well as storage at room temperature, eliminating the need for refrigeration. Study shows that formulation of NP-TIA’s has significant potential in drug delivery and enhanced shelf-life and storage condition.

Keywords

Catharanthus roseus, Terpenoid Indole Alkaloid, Vinblastine, Silver Nanoparticles, LDH, MMT.
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  • Aslam, J., Khan, S.H., Siddiqui, Z.H., Fatima, Z., Maqsood, M., Bhat, M.A., Nasim, S.A., Ilah, A., Ahmad, I.Z., Khan, S.A., Mujib, A. and Sharma, M.P. (2010). Catharanthus roseus (L.) G. Don. An important drug: its applications and production. Pharmacie Globale Internat. J. Comprehensive Pharmacy, 4 : 55-64.

  • Heijden, R.V., Jacobs, D.I., Snoeijer, W., Hallard, D. and Verpoorte, R. (2004). The catharanthus alkaloids: Pharmacogonosy and biotechnology. Curr. Med. Chem., 11 : 607-628.

  • Murataa, J. and De, L.V. (2005). Localization of tabersonine 16-hydroxylase abd 16-OH tabersonine-16-Omethyltransferase to leaf epidermal cells defines them as a major site of precursor biosynthesis in the vindoleline pathway in Catharanthus Ros. Plant J., 44 : 581-594.

  • Noble, C.O., Guo, Z., Hayes, M.E., Marks, J.D., Park, J.W., Benz, C.C., Kirpotin, D.B. and Drummond, D.C. (2009). Characterization of highly stable liposomal and immunoliposomal formulations of vincristine and vinblastine.Cancer Chemother Pharmacol., 64 : 741–751.

  • Noble, R.L. (1990).The discovery of the vinca alkaloidschemotherapeutic agents against cancer. Biochem. & Cell Biol., 68 : 1344–1351.

  • St-pierre, B., Vazquez-Flota, F.A. and De, L.V. (1999). Multicellular compartmentation of Catharanthus roseus alkaloid biosynthesis predicts intercellular translocation of a pathway intermediate. Plant Cell, 11 : 887-900.

  • Tikhomiroff, C. and Jolicoeur, M. (2002). Screening of Catharanthus roseus secondary metabolites by highperformance liquid chromatography. J. Chromatography A, 955 : 87–93.

  • Uchidia, K., Otsuka, H., Kaeko, M., Kataoka, K. and Nagasaki, Y. (2005).A reactive poly(ethylene glycol) layer to achieve specific surface Plasmon resonance sensing with high S/N ratio: the substantial role of a short underbrushed PEG layer in minimizing non-specific adsorption. Analytical Chemistry, 77 : 1075-1080.

  • Za´rate, R., Dirks, R., Heijden, R. and Verpoorte, R. (2001). Terpenoid indole alkaloid profile changes in Catharanthus pusillus during development. Plant Sci., 160 : 971–977.


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  • Assessment of Temperature Stability of Nanoparticle Formulated Vinblastine

Abstract Views: 281  |  PDF Views: 1

Authors

Manar W. Jan
Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Alok Singh
Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Jishnu Naskar
Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India

Abstract


Catharanthus roseus L. (Madagascar periwinkle) is regarded as a rich source of pharmaceutically important terpenoid indole alkaloids(TIAs) which are valued highly due to their wide spectrum of pharmaceutical application. Among these, vinblastine and vincristine are of particular importance because of their wide use in cancer chemotherapy. Vinblastine can have extensive use for the treatment of lung cancer, breast cancer, head and neck cancer and testicular cancer. It is also used to treat Langerhens cell histocytosis. This study was done to assess the stability of vinblastine. The extracted crude vinblastine was formulated with nanoparticles at different temperature. Stability was determined at varying temperature. Reduction method was used to synthesize silver nanoparticles, LDH was synthesized using co-precipitation method and MMT was purchased from Sigma-Aldrich.LDH and MMT formulated vinblastine showed maximum temperature stability at 60oC and 80oC. The increase in heat stability will help in increasing the shelf-life as well as storage at room temperature, eliminating the need for refrigeration. Study shows that formulation of NP-TIA’s has significant potential in drug delivery and enhanced shelf-life and storage condition.

Keywords


Catharanthus roseus, Terpenoid Indole Alkaloid, Vinblastine, Silver Nanoparticles, LDH, MMT.

References