International Journal of Plant Protection

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Study on Biophysical and Biochemical Basis of Shoot and Fruit Borer Tolerance in Brinjal


Affiliations
1 Department of Agriculture, Bhagwant University, Ajmer (Rajasthan), India
2 Department of Agricultural Biotechnology, Bhagwant University, Ajmer (Rajasthan), India
     

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Brinjal or eggplant or aubergine, Solanum melongena L. is one of the most important vegetable crops grown in India and other parts of the world. It is highly cosmopolitan and popular vegetable grown as poor man’s crop in India. Brinjal, being a native to India has great variability existing in the country. However, it is highly infested by shoot and fruit borer, Leucinodes orbonalis Guen., an obnoxious pest causing fruit damage upto 92.5 per cent. The pest is very active during the summer and rainy season and often causes more than 90 per cent damage. Pesticide application is not the only solution of managing the pest as repeated use of pesticide leads to health hazards, destruction of beneficial insects, pest resurgence and environmental pollution. Thus, in order to develop varieties resistant to this pest we need to know the biophysical and biochemical traits that often result intolerance mechanism. Thirty six diverse brinjal genotypes were grown in RBD with three replications at the Vegetable Research Farm, BAC, Sabour during spring-summer season of 2015-16 and morphological and biochemical studies were performed to accomplish these objectives. The findings revealed that sufficient genetic variability was present among the genotypes which provide ample scope for selection of promising genotypes under study. Shoot borer infestation was least in pusa purple cluster (3.28%) followed by BRBR-01 while fruit borer infestation percentage by both number and weight was least in BRBL-01. Pusa purple long was the highest yielder (1100.02g/plant) followed by BRBL-01 (1046.38g/plant). The morphological characters such as average fruit weight, fruit/plant, yield/plant, shoot borer infestation and fruit borer infestation percentage by number possessed high heritability coupled with high genetic advance. All the biochemical characters except leaf chlorophyll and ascorbic acid exhibited high heritability coupled with high genetic advance. Therefore, selection will be more effective for these characters since they are highly heritable and easily fixable due to additive gene effect. D2 cluster analysis grouped the thirty genotypes into 7 clusters. The inter and intra cluster distance between the genotypes under study indicate the existence of higher level of genetic divergence among them. The highest inter cluster distance was observed between cluster III (comprising of BRBL-01) and cluster IV and intra cluster distance was observed within cluster IV which suggest that hybridization between the members of these clusters may lead to creation of higher variability and heterosis. Total fruit anthocyanin had maximum contribution towards genetic divergence (27%), followed by fruit borer infestation percentage by weight (21%), fruit borer infestation percentage by number (18%), polyphenol oxidase activity (15%), leaf anthocyanin (8%), yield/plant (6%) and number of fruits/plant (4%). Trichome density showed highly significant negative correlation with shoot infestation also days to first flowering and 50 per cent flowering exhibited significant negative correlation with shoot infestation and fruit infestation by weight. Days to first flowering and fruit length showed significant and positive correlation with fruit yield/plant. So selection for these two characters can be rewarding for yield and quality improvement in brinjal. Fruit weight and trichome density possessed high direct effect and hence direct selection for these characters can be rewarding for yield and pest resistance improvement. Total phenol content of fruit exerted highly significant and negative correlation with fruit borer infestation by weight and fruit borer infestation percentage by number. So selection for these traits can lead to quality improvement, particularly for pest resistance. The genotypes BRBL-01, pusa purple long, pusa purple cluster and BRBL-04 have been found to be high yielders as well as possessing moderate resistance towards fruit and shoot borer besides having good biochemical properties that may be effective in imparting the resistant trait in the genotypes. Therefore, these superior genotypes may be used in future breeding programme for improvement of yield and especially quality.

Keywords

GCV, PCV, DFF, Cluster, Solanum melongena.
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  • Al- Jibouri, H.A. Millar, P.A. and Robinson, H.F. (1958). Genotypic and environmental variances and co-variance in an upland cotton cross of interspecific origin. Agron. J., 50: 633-637.

  • Amin, S.M.R., Alam, M.Z., Rahman, M.M., Hossain, M.M. and Mian, I.H. (2014). Study on morphological characteristics of leaves, shoots and fruits of selected brinjal varieties/lines influencing brinjal shoot and fruit borer infestation. Internat. J. Econ. Plants, 1(1): 1-8.

  • Apak, R., Guclu, K., Ozyurek, M. and Karademir, S.E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC Method. J. Agric. & Food Chem., 52 (26) : 7970-7981.DOI: PMid: 15612784 .

  • Ardez, K.P., Sumalde, A.C. and Taylo, L.D. (2008). Ovipositional preference, host range and life history of eggplant fruit and shoot borer, Leucinodes orbonalis Guenee (Lepidoptera: Pyralidae). Philippine Entomologist., 22(2): 173-183.

  • Arnon, D. I. (1949). Copper enzymes in isolated chloroplast polyphenol oxidase in Beta vulgaris. Pl. Physiol., 24 : 115.

  • Bharadiya, A.M. and Patel, B.R. (2005). Study on the efficacy of certain insecticides against brinjal shoot and fruit borer, Leucinodes orbonalis Guenee. J. Plant Protec. & Environ., 2 : 113-115.

  • Chandrashekhar, C.H., Malik, V.S. and Singh, R. (2009). Morphological and biochemical factors of resistance in eggplant against Leucinodes orbonalis (Lepidoptera: Pyralididae). Entomologia Generalis, 31: 337-345.

  • Dewey, D.R. and Lu, K.H. (1959). A correlation and path co-efficient analysis of components of crested wheat grass production. Agron. J., 51: 515-518.

  • Dhaka, S.K. and Soni, A.K. (2012).Genetic variability in brinjal (Solanum melongena L.). Asian J. Hort., 7(2): 537-540.

  • Dhruve, J.J., Shah, R., Gandhi, S. and Talati, J.G. (2014). Biochemical and morphological traits of different cultivars of brinjal fruits growing in Anand (Gujarat). Indian J. Agric. Biochem., 27(2): 211-214.

  • Elanchezhyan, K., Muralibaskaran, R.K. and Rajavel, D.S. (2008). Field screening of brinjal varieties on major pests and their natural enemies. J. Bio Pesticides, 1(2):113- 120.

  • Elanchezhyan, K., Muralibaskaran, R.K. and Rajavel, D.S. (2009). Biochemical basis of resistance in brinjal genotypes to shoot and fruit borer, Leucinodes orbonalis Guen. J. Entomological Res., 33: 101-114.

  • Gupta, Y.C. and Kauntey, R.P.S. (2008). Studies on fruit characters in relation to infestation of shoot and fruit borer, Leucinodes orbonalis Guen.in brinjal, Solanum melongena L. J. Entomological Res., 32 (2) : 119-123.

  • Johnson, H.W., Robinson, H.F. and Comstock, R.E. (1955). Estimates of genetic and environmental variability in soybean. Agron. J., 47: 274-318.

  • Khorsheduzzaman, A.K.M., Alam, M.Z. and Rahman, M.M. (2010). Biochemical basis of resistance in eggplant (Solanum melongena L.) to Leucinodes orbonalis Guenee and their correlation with shoot and fruit infestation. Bangladesh J. Agric. Res., 35(1): 149-155.

  • Kumar, S.R., Arumugam, T. and Balakrishan, S. (2013). Studies on evaluation and mean performance associated with yield, quality and pest and disease incidence in eggplant (Solanum melongena L.) Internat. J. Hort., 13(3): 70-79.

  • Kushwah, S. and Bandhyopadhya, B.B. (2005).Variability and Correlation studies in brinjal lndian. J. Hort., 62(2): 210-212.

  • Lush, J.L. (1940). Intra-sire correlations or regressions of offspring on dam as a method of estimating heritability of characteristics. Proc. Amer. Soc. Anim. Prod., 293.

  • Lush, J.L. (1949). Heritability of quantitative characters in farm animals. Heriditas (suppl.). 35 : 256-261.

  • Miller, Daniel (2007). Genetically engineered eggplant. Span, 41.

  • Naliyadhara, M.V., Mehta, D.R., Pandya, H.M. and Purohit, V.L. (2007). A study on genetic diversity and genetic variability in brinjal (Solanum melongena L.). Agric. Sci. Digest., 27 (1): 22-25.

  • Naqvi, A.R., Pareek, B.L., Nanda, U.S. and Mitharwal, B.S. (2008). Leaf morphology and biochemical studies on different varieties of brinjal in relation to major sucking insect pests. Indian J. Plant Protec., 36 (2) : 245-248.

  • Naqvi, A.R., Pareek, B.L., Nanda, U.S. and Mitharwal, B.S. (2009). Biophysical characters of brinjal plant governing resistance to shoot and fruit borer, L. orbonalis. Indian J. Plant Protec., 37(1/2): 1-6.

  • Padgilwar, T., Deshmukh, M. and Mahorkar, V.K. (2009). Correlation and path analysis for biochemical constituents and fruit infestation in brinjal. Plant Archiv., 9(1): 307-308.

  • Pareek, B.L. and Bhargava, M.C. (2009). Estimation of avoidable losses in vegetable crops caused by borers under semi-arid conditions of Rajasthan. Insect Environment., 9: 59-60.

  • Prabakaran, S. (2010). Evaluation of local types of brinjal (Solanum melongena L.). M.Sc., (Hort.) Thesis, Agricultural College and Research Institute, Tmil Nadu Agricultural University, Madurai, T.N. (INDIA).

  • Prabhu, M., Natarajan, S. and Pugalendhi, L. (2007). Biochemical basis of shoot and fruit borer (Leucinodes orbonalis Guen.) resistance in brinjal (Solanum melongena L). In: Keshavachandran, R., Nazeem, P.A., Girija, D., John, P.S. and Peter, K.V. (Ed.), Recent trends in Horticultural Biotechnology, New India Publishing Agency, New Delhi, India, pp. 829-837.

  • Prabhu, M., Natarajan, S., Veeraragavathatham, D. and Pugalendhi, L. (2009). The biochemical basis of shoot and fruit borer resistance in interspecific progenies of brinjal (Solanum melongena). Eur. Asian J. BioSci., 3: 50-57.

  • Prasad, T.V., Bhardwaj, R., Gangopadhyay, K.K., Arivalagan, M., Bag, M.K., Meena, B.L. and Dutta, M. (2014).Biophysical and biochemical basis of resistance to fruit and shoot borer (Leucinodes orbonalis Guennee) in eggplant. Indian J. Hort., 71 (1): 67-71.

  • Ranganna, S. (1977). Plant pigments. In: S. Ranganna (Ed.): Manual of analysis of fruit and vegetable products. Tata McGraw-Hill Publishing Co., Ltd. New Delhi. pp. 72-93.

  • Roychowdhury, R., Datta, S. Gupta, P. and Tah, J. (2012). Analysis of genetic parameters on mutant populations of mungbean (Vigna radiata L.) after ethyl methyl sulphonate treatment. Notulae Scientia Biologicae., 4 (1): 137-143.

  • Samadia, D.K., Aswani, R.C. and Dhandar, G. (2006). Genetic analysis for yield components in tomato land races. Haryana J. Hort. Sci., 35 (1&2): 116-119.

  • Senapati, N., Mishra, H.N., Bhoi, M.K., Dash, S.K. and Prasad, G. (2009). Genetic variability and divergence studies in brinjal (Solanum melongena L.). Veg. Sci., 36 (2): 150-154.

  • Shinde, K.G., Birajdar, U.M., Bhalekar, M.N. and Patil, B.T. (2012). Correlation and path analysis in eggplant (Solanum melongena L.). Veg. Sci., 39(1): 108-110.

  • Singh, A.K., Ahmed, N. and Narayan, S. (2006). Genetic divergence studies in brinjal under temperate condition. Indian J. Hort., 63 (4) : 407-409.

  • Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol., 299 : 152-178.

  • Soundararajan, R. and Baskaran, P. (2005). Effects of antibiosis components of eggplant on the developmental biology of whitefly parasitoids, Encarsia transvena T. and Eretmocerus mundus M. (Aphelinidae: Hymenoptera). In: National seminar on sustainable insect pest management (Ignacimuthu, S. and Jayaraj, S. Ed.), Loyola College, Chennai. pp. 298.

  • Thamburaj, S. and Singh, N. (2013). Vegetables, tubercrops and spices. Indian Council of Agricultural Research, NEW DELHI, INDIA.

  • Thangamani, C. and Jansirani, P. (2012). Correlation and path co-efficient analysis studies on yield and attributing characters in brinjal (Solanum melongena L.). Electronic J. Plant Breed., 3 (3): 939-944.

  • Wagh, S.S., Pawar, D.B., Chandele, A.G. and Ukey, N.S. (2012). Biophysical mechanisms of resistance to brinjal shoot and fruit borer, Leucinodes orbonalisGuenee in brinjal. Pest Mgmt. Hort. Ecosyst., 18 (1) : 54-59.

  • Warade, S.D., Shinde, K.G. and Kadam, J.H. (2008). Association of characters in relation to shoot and fruit borer infestation in brinjal (Solanum melongena L.). Asian J. Hort., 3 : 304-306.

  • Wright, S. (1921). Correlation and causation. J. Agirc.Res., 20: 557 - 585.

  • Yadav, D.S. and Sharma, M.M. (2005). Evaluation of brinjal varieties for their resistance against fruit and shoot borer, Leucinodes orbonalis Guenne. Indian J. Entomol., 67(2): 129-132.


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  • Study on Biophysical and Biochemical Basis of Shoot and Fruit Borer Tolerance in Brinjal

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Authors

Shubham Shukla
Department of Agriculture, Bhagwant University, Ajmer (Rajasthan), India
Devendra Singh
Department of Agriculture, Bhagwant University, Ajmer (Rajasthan), India
Birbal Bairwa
Department of Agriculture, Bhagwant University, Ajmer (Rajasthan), India
Bhanwar Lal Jat
Department of Agricultural Biotechnology, Bhagwant University, Ajmer (Rajasthan), India

Abstract


Brinjal or eggplant or aubergine, Solanum melongena L. is one of the most important vegetable crops grown in India and other parts of the world. It is highly cosmopolitan and popular vegetable grown as poor man’s crop in India. Brinjal, being a native to India has great variability existing in the country. However, it is highly infested by shoot and fruit borer, Leucinodes orbonalis Guen., an obnoxious pest causing fruit damage upto 92.5 per cent. The pest is very active during the summer and rainy season and often causes more than 90 per cent damage. Pesticide application is not the only solution of managing the pest as repeated use of pesticide leads to health hazards, destruction of beneficial insects, pest resurgence and environmental pollution. Thus, in order to develop varieties resistant to this pest we need to know the biophysical and biochemical traits that often result intolerance mechanism. Thirty six diverse brinjal genotypes were grown in RBD with three replications at the Vegetable Research Farm, BAC, Sabour during spring-summer season of 2015-16 and morphological and biochemical studies were performed to accomplish these objectives. The findings revealed that sufficient genetic variability was present among the genotypes which provide ample scope for selection of promising genotypes under study. Shoot borer infestation was least in pusa purple cluster (3.28%) followed by BRBR-01 while fruit borer infestation percentage by both number and weight was least in BRBL-01. Pusa purple long was the highest yielder (1100.02g/plant) followed by BRBL-01 (1046.38g/plant). The morphological characters such as average fruit weight, fruit/plant, yield/plant, shoot borer infestation and fruit borer infestation percentage by number possessed high heritability coupled with high genetic advance. All the biochemical characters except leaf chlorophyll and ascorbic acid exhibited high heritability coupled with high genetic advance. Therefore, selection will be more effective for these characters since they are highly heritable and easily fixable due to additive gene effect. D2 cluster analysis grouped the thirty genotypes into 7 clusters. The inter and intra cluster distance between the genotypes under study indicate the existence of higher level of genetic divergence among them. The highest inter cluster distance was observed between cluster III (comprising of BRBL-01) and cluster IV and intra cluster distance was observed within cluster IV which suggest that hybridization between the members of these clusters may lead to creation of higher variability and heterosis. Total fruit anthocyanin had maximum contribution towards genetic divergence (27%), followed by fruit borer infestation percentage by weight (21%), fruit borer infestation percentage by number (18%), polyphenol oxidase activity (15%), leaf anthocyanin (8%), yield/plant (6%) and number of fruits/plant (4%). Trichome density showed highly significant negative correlation with shoot infestation also days to first flowering and 50 per cent flowering exhibited significant negative correlation with shoot infestation and fruit infestation by weight. Days to first flowering and fruit length showed significant and positive correlation with fruit yield/plant. So selection for these two characters can be rewarding for yield and quality improvement in brinjal. Fruit weight and trichome density possessed high direct effect and hence direct selection for these characters can be rewarding for yield and pest resistance improvement. Total phenol content of fruit exerted highly significant and negative correlation with fruit borer infestation by weight and fruit borer infestation percentage by number. So selection for these traits can lead to quality improvement, particularly for pest resistance. The genotypes BRBL-01, pusa purple long, pusa purple cluster and BRBL-04 have been found to be high yielders as well as possessing moderate resistance towards fruit and shoot borer besides having good biochemical properties that may be effective in imparting the resistant trait in the genotypes. Therefore, these superior genotypes may be used in future breeding programme for improvement of yield and especially quality.

Keywords


GCV, PCV, DFF, Cluster, Solanum melongena.

References