International Journal of Plant Protection

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Cultural and Morphological Variations among the Isolates of Sclerotinia sclerotiorum (Lib.) De Bary Causing Sclerotinia Stem Rot


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1 Department of Plant Protection, Aligarh Muslim University, Aligarh (U.P.), India
     

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Sclerotinia stem rot caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most serious and damaging disease of many important crops and weeds worldwide. It causes considerable yield losses in quality and quantity of crop produce. In present study, Eleven isolates of Sclerotinia sclerotiorum were collected from different host plants and studied for their cultural and morphological variations. The observations of study revealed that all isolates varied in their cultural characters viz., colony growth (mm), colony appearance (colour and type of growth) and morphological characters viz., initiation, size and number of sclerotia. Among different isolates, carrot isolate (75.86 mm) was found significantly fast growing followed by brinjal isolate (70.96 mm), while potato isolate (57.13 mm) grown slowly in potato dextrose agar medium at 72 hours after inoculation. Maximum number of sclerotia (34.66) was produced in tomato isolate, whereas, minimum (4.33) in parthenium isolate. Largest size of sclerotia (9x4.66 mm) was observed in tomato isolate however, smallest size of sclerotia (2.0x2.0 mm) were produced in lentil and parthenium isolates.

Keywords

Sclerotinia sclerotiorum, Isolates, Cultural, Morphological Variations.
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  • Akram, S. H., Iqbal, M., Ahmed, N., Iqbal, U. and Ghafoor, A. (2008). Morphological variability and mycelia compatibility among the isolates of Sclerotinia sclerotiorum associated with stem rot of chickpea. Pak. J. Bot., 40 (6) : 2663-2668.

  • Boland, G. J. and Hall, R. (1994). Index of plant hosts of Sclerotinia sclerotiorum. Can. Jan. Plant Pathol.,16 : 93-108.

  • Farr, D. F and Rossman, A.Y. (2011). Sclerotinia sclerotiorum. In: Fungal databases, systematic mycology and microbiology laboratory. United States Department of Agriculture, Agricultural Research Service.

  • Ghasolia, R. P. and Shivpuri, A. (2007). Morphological and pathogenic variability in Rapeseed and mustard isolates of Sclerotinia sclerotiorum. Indian Phytopath., 60 : 76-81.

  • Kohn, L. M., Stasovski, E., Carbone, I., Royer, J. and Anderson, J. B. (1991).Mycelial incompatibility and molecular markers identify genetic variability in field populations of S. sclerotiorum. Phytopathology, 81: 480-485.

  • Li, C.X., Li, H., Sivasithamparam, K., Fu, T.D., Li, Y.C., Liu, S.Y. and Barbetti, M.J. (2006). Expression of field resistance under Western Australian conditions to Sclerotinia sclerotiorum in Chinese and Australian Brassica napus and Brassica juncea germplasm and its relation with stem diameter. Australian J. Agril. Res., 57:1131–1135.

  • Manjunatha, N., Devi, Prameela T., Prabhakaran, Gurudevi V. N., Navali, I.S. and Swathi, P. (2014).Morphiological and molecular diversity of Sclerotinia sclerotiorum de Bary isolates of India. Bioscan., 9(4): 1763-1767.

  • Purdy, L. H. (1979). Sclerotinia sclerotiorum: History, diseases, and symptom pathology, host range, geographic distribution and impact. Phytopathology, 69: 875-880.

  • Upadhyay, P., Tiwari, A. K. and Bisht, K. S. (2015).Cultural, morphological, pathogenic variability and mycelial compatibility among the isolates of sclerotinia sclerotiorum (Lib.) De Bary cause of sclerotinia rot. Bioscan, 10(4): 1813-181.

  • Zhao, J., Peltier, A. J., Meng, J., Osborn, T. C. and Grau, C. R. (2004). Evaluation of Sclerotinia stem rot resistance in oilseed Brassica napus using a petiole inoculation technique under green house conditions. Plant Dis., 88: 1033–1039.


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  • Cultural and Morphological Variations among the Isolates of Sclerotinia sclerotiorum (Lib.) De Bary Causing Sclerotinia Stem Rot

Abstract Views: 225  |  PDF Views: 0

Authors

Devesh Pathak
Department of Plant Protection, Aligarh Muslim University, Aligarh (U.P.), India
R. U. Khan
Department of Plant Protection, Aligarh Muslim University, Aligarh (U.P.), India
Vaibhav Pratap Singh
Department of Plant Protection, Aligarh Muslim University, Aligarh (U.P.), India

Abstract


Sclerotinia stem rot caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most serious and damaging disease of many important crops and weeds worldwide. It causes considerable yield losses in quality and quantity of crop produce. In present study, Eleven isolates of Sclerotinia sclerotiorum were collected from different host plants and studied for their cultural and morphological variations. The observations of study revealed that all isolates varied in their cultural characters viz., colony growth (mm), colony appearance (colour and type of growth) and morphological characters viz., initiation, size and number of sclerotia. Among different isolates, carrot isolate (75.86 mm) was found significantly fast growing followed by brinjal isolate (70.96 mm), while potato isolate (57.13 mm) grown slowly in potato dextrose agar medium at 72 hours after inoculation. Maximum number of sclerotia (34.66) was produced in tomato isolate, whereas, minimum (4.33) in parthenium isolate. Largest size of sclerotia (9x4.66 mm) was observed in tomato isolate however, smallest size of sclerotia (2.0x2.0 mm) were produced in lentil and parthenium isolates.

Keywords


Sclerotinia sclerotiorum, Isolates, Cultural, Morphological Variations.

References