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Evaluation of Various Aqua Suspension Formulations of Beauveria bassiana (Balsamo) Vuillemin against Helicoverpa armigera


Affiliations
1 Department of Entomology, College of Agriculture, Latur (M.S.), India
2 Agricultural Research Station, Niphad, Nashik (M.S.), India
     

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Studies on laboratory evaluation of nine aqua suspension formulations of entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin comprising adjuvants viz. 1) B.b.+GLY+CMC, 2) B.b.+GLY+HO, 3) B.b.+SFO+CMC 4) B.b.+SFO+HO 5) B.b.+GLY+BA, 6) B.b.+GLY+BA+TW, 7) B.b.+SFO, 8) B.b.+GNO and 9) Control (B.b. alone) were carried out against the II and III instar larvae of H.armigera. The data on larval mortality was recorded at 5, 7 and 10 days after treatment (DAT). At 10 DAT all the formulations were significantly superior to control (B.b. alone) for the lethal effect. The formulation B.b.+ SFO + CMC registered significantly highest larval mortality of 90.00 and 83.33 per cent of II and III instar larvae of H.armigera, respectively. However, it was at par to the formulation B.b.+ GLY + HO recording 86.67 and 80.00 per cent mortality of II and III instar larvae of H.armigera, respectively. The control (B. b. alone) recorded lowest (63.33 and 53.33 %) mortality of the caterpillar.

Keywords

Beauveria bassiana, Adjuvants, Glycerol (GLY), Honey (HO), Sunflower Oil (SFO), Groundnut Oil (GNO), Tween 80 (TW), Carboxymethyl Cellulose (CMC), Boric Acid (BA).
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  • Bassi, A. (1835). Del mal del sengo, Calcinaccio, O. Mascardino, Malattia Cheafflige I bacci da Serta, E sul mado dil Li 61 Libernarnele le bigattiaque anche le piu infestanate, Tip Orcesi, Iodf. Biotech. Italliana., 78: 246-248.
  • Devaprasad, V., Jayaraj, S. and Rabindra, R.J. (1989). Susceptibility of tobacco caterpillar, S. litura (F.) to certain entomopathogenous fungi. J. Biol. Control., 3:53-55.
  • Devaprasad, V., Jayraj, S. and Rabindra, R.J. (1990). Susceptibility of gram caterpillar, Heliothis armigera Hbn. (Noctuidae : Lepidoptera) to certain entomopathogenic fungi. J.Biol. Control., 4(1): 44-47.
  • Gomez, K.A. and Gomez, A. (1984). Statistical procedure for agricultural research, Hand Book. John Wiley & Sons, NEW YORK, U.S.A.
  • Gopalakrishnan, C. and Narayanan, K. (1990). Studies on the dose-mortality relationship between the entomofungal pathogen Beauveria bassiana (Bals.) Vuillemin and Heliothis armigera Hubner (Lepidoptera: Noctuidae). J. Biol. Control., 4 (2): 112-115.
  • Hajek, A.E. and St.Leger, R. J. (1994). Interactions between fungal pathogen and insect hosts.Ann. Rev. Ent., 39: 293-322.
  • Hassani, M. (2000). Development and proving of biocontrol method based on Bacillus thuringiensis and entomopathogenic 64 fungi against the cotton pests Spodoptera littoralis (Boisd.),H. armigera (Hubner) Hardwick (Noctuidae: Lepidoptera). A Thesis, degree of Doctor in Agricultural science of the Justus Liebig University, Giessen, Germany.
  • Kaur, G., Padmaja, V. and Sasikala, V. (1999). Control of insect pests on cotton through mycopesticide formulations. Indian J. Microbiol., 39 : 169-173.
  • Nahar, P., Yadav, P., Kulye, M., Hadapad, A., Hassani, M., Tuor, U., Keller, S., Chandele, A.G., Thomas, B. and Deshpande, M.V. (2004). Evaluation of indigenous fungal isolates, Metarhizium anisopliae M34412, Beauveria bassiana B3301 and Nomuraea rileyi N812 for the control of Helicoverpa armigera (Hubner) in pigeonpea field. J. Biol. Control., 18 (1) : 1-7.
  • Narayanan, K. (1988).Microbial control ofHeliothis armigera (Hub.) Tech. Bull., I.I.H.R., Bangalore. pp. 28.
  • Nirmala, R., Ramanujam, B., Rabindra, R.J. and Rao, N.S. (2005). Growth parameter of some isolates of entomofungal pathogen and production of dust free spores on rice medium. J. Biol. Control., 19 (2): 121-128.
  • Nirmala, R., Ramanujam, B., Rabindra, R.J. and Rao, N.S. (2006). Effect of entomofungal pathogens on mortality of three aphid species. J. Biol. Control., 20(1): 89-94.
  • Pandey, A.K. and Kanaujia, K.R. (2005). Effect of different grain media on sporulation, germination and virulence of Beauverai bassiana (Bals.) Vuill. against Spodoptera litura Fab. Larvae. J. Biol. Control., 19 (2) : 129-133.
  • Parmar, S.J. (2001). Studies on mass multiplication and formulation of B. bassiana as a biopesticide. M.Sc. Thesis, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, M.S. (INDIA).
  • Ramkumar (1998). Studies on integration of entomopathogens for the management of S. litura (Fab.) (Lepidoptera: Noctuidae) on cabbage. M.Sc. (Ag.) Thesis, Univecity of Agricultural Sciences, Bangalore, KARNATAKA (INDIA).
  • Steinhaus, E.A. (1965). Microbial diseases of insects. In: Biological Control of Insect Pests and Weeds. (Ed.) Paul DeBach, Reinhold Publishing Corp, New York. pp. 515-547.
  • Udar, P. V. (2002). Pathogenic studies of B. bassiana (Balsamo) Vuillemin against H. armigera (Hubner) Hardwick. M.Sc. Thesis, Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola, M.S. (INDIA).
  • Vimaladevi, P. S. and Hari, P. P. (2009). Identification of virulent isolate of the entomopathogenic fungus B. bassiana (Balsamo) Vuillemin, its mass multiplication and formulation for development into a mycoinsecticide for management of Helicoverpa armigera (Hubner). J.Biol.Control., 23(2):137-144.

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  • Evaluation of Various Aqua Suspension Formulations of Beauveria bassiana (Balsamo) Vuillemin against Helicoverpa armigera

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Authors

R. S. Jadhav
Department of Entomology, College of Agriculture, Latur (M.S.), India
S. D. Patil
Agricultural Research Station, Niphad, Nashik (M.S.), India

Abstract


Studies on laboratory evaluation of nine aqua suspension formulations of entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin comprising adjuvants viz. 1) B.b.+GLY+CMC, 2) B.b.+GLY+HO, 3) B.b.+SFO+CMC 4) B.b.+SFO+HO 5) B.b.+GLY+BA, 6) B.b.+GLY+BA+TW, 7) B.b.+SFO, 8) B.b.+GNO and 9) Control (B.b. alone) were carried out against the II and III instar larvae of H.armigera. The data on larval mortality was recorded at 5, 7 and 10 days after treatment (DAT). At 10 DAT all the formulations were significantly superior to control (B.b. alone) for the lethal effect. The formulation B.b.+ SFO + CMC registered significantly highest larval mortality of 90.00 and 83.33 per cent of II and III instar larvae of H.armigera, respectively. However, it was at par to the formulation B.b.+ GLY + HO recording 86.67 and 80.00 per cent mortality of II and III instar larvae of H.armigera, respectively. The control (B. b. alone) recorded lowest (63.33 and 53.33 %) mortality of the caterpillar.

Keywords


Beauveria bassiana, Adjuvants, Glycerol (GLY), Honey (HO), Sunflower Oil (SFO), Groundnut Oil (GNO), Tween 80 (TW), Carboxymethyl Cellulose (CMC), Boric Acid (BA).

References