International Journal of Plant Protection

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Efficacy of Bio-Agents against Damping Off in Solanaceous Crops under Nursery Conditions


Affiliations
1 Krishi Vigyan Kendra (I.G.K.V.), Sarkanda Farm, Bilaspur (C.G.), India
2 T.C.B. College of Agriculture and Research Station (I.G.K.V.), Sarkanda, Bilaspur (C.G.), India
3 S.K. College of Agriculture, Kabirdham, Kawardha (C.G.), India
     

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The study was conducted to assess efficacy of Trichoderma harzianum and Pseudomonas fluorescens against damping off in tomato and brinjal seedlings during Kharif season. The investigation was performed as on farm trials on farmers’ field over three years viz., 2013-14, 2014-15 and 2015-16. T. harzianum and P. fluorescens were applied in combination of soil+seed treatment. Talc based formulation of both bio control agents were used @ 10 g kg-1 in seed treatment and 100 g m-2 in soil treatment. Results indicated that the combination of soil+seed treatment with T. harzianum was gives maximum seedling stands (84.15%) with minimum mortality (15.85%) in brinjal followed by soil+seed treatment with mixture of T. harzianum and P. fluorescens (78.30%) and soil+seed treatment with P. fluorescens alone (69.68%) whereas, in control plot treated with Carbendazim 50 WP (soil+seed) recorded less seedling stand (41.50%). In case of tomato, application of T. harzianum as soil+seed gave the maximum seedling stand (80.32%) followed by soil+seed treatment with combination of T. harzianum and P. fluorescens (73.45%) and P. fluorescens alone (58.64%) while seed+soil treatment with Carbendazim 50 WP given minimum seedling stand (42.01%). Consortium of T. harzianum and P. fluorescens significantly increased the growth and yield attributes of brinjal and tomato crops i.e. plant height, number of branches plant-1, number of fruit plant-1 and fruit yield ha-1 followed by along Trichoderma and Pseudomonas. The study showed that T. harzianum and P. fluorescens are the potential bio control agents as compare to Carbendazim 50 WP and can be used effectively for the management of damping off in seedlings in nursery.

Keywords

Tomato, Brinjal, Bio-Agents, Damping Off, Pseudomonas, Trichoderma.
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  • Bai, Z., Jin, B., Li, Y., Chen, J. and Li, Z. (2008).Utilization of winery wastes for Trichoderma viride bio-control agent production by solid state fermentation. J. Environ. Sci., 20 : 353 -358.

  • Champawat, R.S. and Sharma, R.S. (2003). Integrated management of nursery disease in brinjal, chilli, cabbage and onion. J. Myco. Pl. Pathol., 33(2): 290-291.

  • Dar, G.H., Hassan, M.G., Humaira, R., Dar, W.A. and Misba, M. (2015). Evaluation of microbial antagonists for the management of wilt/ischolar_main rot and damping-off diseases in chilli (Capsicum annuum). Vegetos-An Int. J. Pl. Res., 28(4): 102-110.

  • El-Katatny, M.H., Gudelij, M., Robra, K.H., Elnaghy, M.A. and Gubitz, G.M. (2001). Characterization of a chitinase and an endo-b-1, 3-glucanase fromTrichoderma harzianum Rifai T24 involved in control of the phytopathogen Sclerotium rolfsii. App. Micro. Biotech., 56:137-143.

  • Gargi, C. and Kalita, M.C. (2012). Biocontrol potential of Pseudomonas fluorescens against bacterial wilt of Brinjal and its possible plant growth promoting effects. Ann. Bio. Res., 3(11) : 5083-5094.

  • Ghildiyal, A. and Pandey, A. (2008). Isolation of cold tolerant antifungal strains of Trichoderma species from glacial sites of Indian Himalayan region. Res. J. Micro., 8: 559-564.

  • Gholve, V.M., Tatikundalwar, V.R., Suryawanshi, A.P. and Dey, U. (2014). Effect of fungicides, plant extracts/botanicals and bioagents against damping off in brinjal. Afri. J. Micro. Res., 8 (30) : 2835-2848.

  • Gomathi, A., Ambikapathy, V. and Pannerselvam, A. (2011). Potential strain of Trichoderma spp. to control damping off of disease in chilli. Int. J. App. Bio. Pharma. Tech., 2(4): 64-69.

  • Harman, G.E., Howell, C.R., Viterbo, A., Chet, I. and Lorito, M. (2004). Trichoderma species-opportunistic, virulent plant symbionts. Nature reviews micro., 2(1): 43-56.

  • Harman, G.E. (2011).Multifunctional fungal plant symbionts: new tools to enhance plant growth and productivity. New phyto. Commen. Forum., 3: 647-649.

  • Jena, N. (2012). Pseudomonas and Trichoderma: The most effective bio-control agents against damping off pathogens of vegetable crops. Asian J. Micro. biotech. Enviro. Sci., 14(2): 297-300.

  • Karpagavalli, S. and Ramabadran, R. (2001). Effect of fungicides and Trichoderma species on cellulolytic enzyme production, damping-off incidence and seedling vigour of tomato. Pl. Dis. Res., 16: 179 185.

  • Muddin, M., Akther, N., Islam, M.T. and Farug, N.A. (2011). Effect of Trichoderma harzanium and some soil amendment against damping-off disease complex of potato and chilli. Agriculturi., 9(1-2): 106-116.

  • Muthukumar, A., Eswaran, A. and Sanjeevkumas, K. (2011). Exploitation of Trichoderma species on the growth of Pythium aphanidermatum in chilli. Braz. J. Micro., 42: 1598-1607.

  • Olawumi I. O, Christopher O. A. and Olusola, S.A. (2016). The interactive effects of three Trichoderma species and damping off causative pathogen Pythium aphanidermatum on emergence indices, infection incidence and growth performance of sweet pepper. Int. J. Rec. Scien. Res., 7(4): 10339-10347.

  • Pandey, K.K. and Pandey, P.K. (2002).Survey and surveillance of vegetable growing area for prevalence of major diseases. Veg. Sci., 30(2): 128-134.

  • Pandey, K.K., Pandey, P.K. and Mishra, K.K. (2004). Integrated disease management of vegetable nursery. Veg. Sci., 31: 45-50.

  • Pandey, P.K., Pandey, K.K., Mishram K.K. and Kumar, A. (2003). Integration effect of different pesticides with Trichoderma viride on seed and seedling germination of different vegetables in nursery. National symposium on sustainable pest management, BHU, Varanasi, 3-5 January, 120121.

  • Savazzini, F., Longa, C.M.O. and Pertot, I. (2009). Impact of the biocontrol agent Trichoderma atroviride SC1 on soil microbial communities of a vine yard in northern mycoparasite antagonism by Trichoderma harzianum. J. Bact., 178: 6382-6385.

  • Shabir, R. and Rubina, L. (2010). Biological control of damping off disease of cabbage caused by Rhizoctonia solani Kuchn. App. Bio. Res., 12: 38 41.

  • Singh, B.N., Singh, A., Singh, S.P. and Singh, H.B. (2011). Trichoderma harzianum mediated reprogramming of oxidative stress response in ischolar_main apoplast of sunflower enhances defence against Rhizoctonia solani. Euro. J. Pl. Patho., 131 (1) : 121-134.

  • Singh, R. and Singh, P.P. (2006). Integrated management of collar rot of Amorphophallus paeoniifolius Blume caused by Sclerotium rolfsii Saccaedo. Veg. Sci., 33(1): 45 49.

  • Singh, S.P., Singh, H.B. and Singh, D.K. (2013). Trichoderma harzianum and pseudomonas sp. Mediated Management of sclerotium rolfsii rot in tomato (lycopersicon esculentummill.). The Bioscan, 8(3): 801-804.

  • Srivastava, M., Vipul Kumar, Shahid, M., Pandey, S. and Singh, A. (2016).Trichoderma - a potential and effective bio fungicide and alternative source against notable phytopathogens: A review. Afri. J. Agric. Res.,11(5):310-316.

  • Srivastava, R., Khalid, A., Singh, U.S. and Sharma, A.K. (2010). Evaluation of arbuscular mycorrhizal fungus, Pseudomonas fluorescent and Trichoderma harzianum formulation against Fusarium oxysporum F. sp. Lycopersici for the management of tomato wilt. Bio. Con., 53 (1): 24-31.

  • Srivastava, V.K. (2004). Trichoderma sp. -a boon for better crop health. Pestol., 28(8): 40- 45.

  • Stephan, Z.A., Jbara, I.M. and Al-Rawi, F.A. (2003). Effect of soil moisture content and thermal treatment on the activity of bioagent fungi Trichoderma harzianum Rifai and Paecilomayces lilacinus (thom) Samson on some tomato plant growth parameters. Ara. J. Pl. Prot., 21(1): 1- 5.

  • Subash, N., Meenakshisundaram, M., Unnamalai, N. and Sasikumar (2013). Effect of Trichoderma harizanum to control damping off disease and growth promotion in chilli (Capsicum annuum). Int. J. Pharma Bio. Sci., 4(2):1076 – 1082.

  • Uddin, M.M., Akhtar, N., Islam, M.T. and Faruq, A.N. (2011). Effect of Trichoderma harzianum and some selected soil amendment against damping off disease complex of potato and chilli. Agricultural, 9(1-2): 106-116.

  • Verma, M., Brar, S.K., Tyagi, R.D., Sahai, V., Prevost, D., Valero J.R. and Surampalli, R.Y. (2007). Bench-scale fermentation of Trichoderma viride on wastewater sludge: rheology, lytic enzymes and biocontrol activity. Enzy. Micro. Tech. 41: 764-771.

  • Zagade, S.N., Deshpande, G.D., Gawade, D.B., Atnoorkar, A.A. and Pawar, S.V. (2012).Biocontrol agents and fungicides for management of damping off in chilli.World J. Agril. Sci., 8 (6): 590-597.


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  • Efficacy of Bio-Agents against Damping Off in Solanaceous Crops under Nursery Conditions

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Authors

V. K. Nirmalkar
Krishi Vigyan Kendra (I.G.K.V.), Sarkanda Farm, Bilaspur (C.G.), India
R. K. S. Tiwari
T.C.B. College of Agriculture and Research Station (I.G.K.V.), Sarkanda, Bilaspur (C.G.), India
S. Singh
S.K. College of Agriculture, Kabirdham, Kawardha (C.G.), India

Abstract


The study was conducted to assess efficacy of Trichoderma harzianum and Pseudomonas fluorescens against damping off in tomato and brinjal seedlings during Kharif season. The investigation was performed as on farm trials on farmers’ field over three years viz., 2013-14, 2014-15 and 2015-16. T. harzianum and P. fluorescens were applied in combination of soil+seed treatment. Talc based formulation of both bio control agents were used @ 10 g kg-1 in seed treatment and 100 g m-2 in soil treatment. Results indicated that the combination of soil+seed treatment with T. harzianum was gives maximum seedling stands (84.15%) with minimum mortality (15.85%) in brinjal followed by soil+seed treatment with mixture of T. harzianum and P. fluorescens (78.30%) and soil+seed treatment with P. fluorescens alone (69.68%) whereas, in control plot treated with Carbendazim 50 WP (soil+seed) recorded less seedling stand (41.50%). In case of tomato, application of T. harzianum as soil+seed gave the maximum seedling stand (80.32%) followed by soil+seed treatment with combination of T. harzianum and P. fluorescens (73.45%) and P. fluorescens alone (58.64%) while seed+soil treatment with Carbendazim 50 WP given minimum seedling stand (42.01%). Consortium of T. harzianum and P. fluorescens significantly increased the growth and yield attributes of brinjal and tomato crops i.e. plant height, number of branches plant-1, number of fruit plant-1 and fruit yield ha-1 followed by along Trichoderma and Pseudomonas. The study showed that T. harzianum and P. fluorescens are the potential bio control agents as compare to Carbendazim 50 WP and can be used effectively for the management of damping off in seedlings in nursery.

Keywords


Tomato, Brinjal, Bio-Agents, Damping Off, Pseudomonas, Trichoderma.

References