International Journal of Plant Protection

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Growth Promotion and Mildew Suppressive Effect of Phylloplane Bacteria of Mulberry (Morus Spp.)


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1 Central Sericultural Research and Training Institute, Srirampuram, Mysuru (Karnataka), India
     

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Studies were conducted to evaluate growth promotion and mildew (Phyllactinia corylea) suppressive effect of phylloplane bacteria of mulberry (Morus spp.) in vitro and in vivo and the effective bacteria were identified. Among 18 bacteria, 4 isolates showed highly significant (P<0.01) reduction with >70 per cent suppression of conidial germination. The highest reduction was showed by the isolate Pb-5 (13.87%) by suppressing 86.13 per cent conidial germination followed by Pb-4 (17.23%) with 82.17 per cent. Similarly, six isolates significantly increased the seed germination. Significantly high (P<0.01) seed germination was obtained (93.44%) with treatment of the isolate Pb- 6 followed by Pb-3 (86.22%), Pb-7 (86.22%) and Pb-4 (85.47%).Most effective bacterial isolates were identified as Bacillus megaterium (Pb-1) Bacillus subtilis (Pb-1) Bacillus cereus (Pb-1) and Pseudomonas aeruginosae (Pb-1). In vivo studies revealed highly significant (P<0.01) with >60% reduction of disease severity with the application of B.megaterium (63.42%) and B.cereus (60.73%). The study suggests exploration of either of these bacteria for biological control of mildew in mulberry.

Keywords

Biological Control, Mulberry, Phylloplane Bacteria, Powdery Mildew.
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  • Akter, S., Kadir, J., Juraimi, A.S., Soud, H. M. and Elmahdi, S. (2014). Isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight. J. Environ. Biol., 35: 1095-1100.

  • Ananthi, M., Selvaraju, P. and Sundaralingal, K. (2014).Effect of bio-priming using bio-control agents on seed germination and seedling vigour in chilli (Capsicum annuum L.) ‘PKM 1’. J. Hort. Sci. & Biotechnol., 89: 564-568

  • Arnold, A.E., Mejía, L.C., Kyllo, D., Rojas, E.I., Maynard, Z., Robbins, N. and Herre, E.A. (2003). Fungal endophytes limit pathogen damage in a tropical tree. Proc. Natl. Aca. Sci., USA. 100 : 15649–15654.

  • Baker, K.F. and Cook, R.J. (1982). Biological control of plant pathogens. The American Phytopathological Society, St.paul MN, pp.433.

  • Bhatt, R.M., Selvakumar, G., Upreti, K.K. and Boregowda, P.C. (2015). Effect of biopriming with enterobacter strains on seed germination and seedling growth of tomato (Solanum lycopersicum L.) under osmotic stress. Proc. Natl. Acad. Sci., India, 85 (1) : 63–69.

  • Biswas, S., Mandal, S.K. and Chinya, P.K. (1993). Development of powdery mildew disease in mulberry and its control. Sericologia, 33 : 653-662.

  • Braun, S.D., Hofmann, J., Wensing, A., Weingart, H., Ullrich, M.S. and Spiteller, D. (2010). In vitro antibiosis by Pseudomonas syringae Pss22d, acting against the bacterial blight pathogen of soybean plants, does not influence in planta biocontrol. J. Phytopathol., 158 : 288-295.

  • Bulgarelli, D., Schlaeppi, K., Spaepen, S., Themaat, E. and Lefert, P. (2013). Structure and functions of the bacterial microbiota of plants. Annu. Rev. Plant Biol., 64 : 807-838.

  • Cappuccino, J.G. and Sherman, N. (2005). Microbiology- A Laboratory Manual, 7th Edition Benjamin Cummings : Pearson Educati on, Inc. New York, U.S.A.

  • Collins, C.N. and Lyne, P.M. (1984).Microbiological methods (6th Ed.), Butterworths, London, United Kingdom.

  • Dandin, S.B, Jaswal, J. and Giridhar, K. (2003). Handbook of sericulture technologies. Central Silk Board, Bangalore, India, pp. 1-259.

  • Deepika, C., Rakesh, K., Khushboo, S., Rashmi and Kumari, Anju (2017). Phyllospheric microflora and its impact on plant growth: A review. Agric. Rev., 38 (1): 51-59

  • Dubey, R.C. and Maheshwary, D.K. (2005). Practical microbiology. S. Chand and Company Ltd., New Delhi, India. pp. 351.

  • Farooq, M., Basara, S.M.A., Hafeez, K. and Ahmad, N. (2009). Thermal hardening: a new seed vigor enhancement tool in rice. J. Integer. Plant Biol., 47 : 187-193.

  • Fatima, B., Yasir, R. and Hasnain, S. (2016). Phylloplane associated plant bacteria of commercially superior wheat varieties exhibit superior plant growth promoting abilities. Frontiers in Life Sci., 9 (4) : 313-322.

  • ISTA (1999). International rules for seed testing: The germination test. Seed Sci. Technol., 27 : 27-32.

  • Kishore, G.K., Pande, S., Podile, A.R. (2005). Phylloplane bacteria increase seedling emergence, growth and yield of field-grown groundnut (Arachis hypogaea L.). Lett. Appl. Microbiol., 40 (4): 260-268.

  • Kong, G.A., Kochman, J.K. and Brown, J.F. (1997). Phylloplane bacteria antagonistic to the sunflower pathogen Alternaria helianthi. Australasian Plant Pathol., 26(2): 85-97.

  • Krishna, A., Mishra, S.P. and Singh, H. (1987). Thickness of cuticle and epidermis in relation to slow powdery mildewing in pea. Indian Phytopath., 40: 218-221.

  • Lautenberg, B.J.J. and Dekkers, L.C. (2002). What makes pseudomonas bacteria rhizopsphere comprtent? Environ. Microbiol., 1 (1) : 913-1011.

  • Maji, M.D., Chattopadhyay, S., Pratheesh Kumar, P.M., Raje Urs, S. (2003a). Evaluation of mulberry phylloplane bacteria for biological control of powdery mildew of mulberry caused by Phyllactinia corylea. J. Mycopathological Res., 41 (2):197-200.

  • Maji, M.D., Quadri, S.M.H. and Pal, S.C. (2003b). Evaluation of mulberry phylloplane microorganism for biological control of Bacterial leaf spot of mulberry caused by Xanthomonas campestris pv. mori, Indian J. Agric. Res., 37 (4) : 307-309.

  • Patro, T.S.S.K., Bahadur, P. and Jindal, J.K. (2002). Exploitation of antagonistic phylloplane bacteria for management of bacterial leaf spot of mung bean. Tropical. Agric. Res., 14 : 174-184.

  • Pratheesh Kumar, P.M., Qadri, S.M.H., Pal, S.C., Mishra, A.K. and Raje Urs, S. (2003). Post infection physiobiochemical alteration at various intensities of leaf spot (Myrothecium roridum) in mulberry. Internat. J. Industrial Entomol.,7(2): 175-180.

  • Pratheesh Kumar, P.M. and Sivaprasad, V. (2017).Fluorescent pseudomonas induced systemic resistance to powdery mildew in mulberry (Morus spp.). Internat. J. Industrial Entomol., 35 (2) : 1-8.

  • Qadri, S.M.H., Pratheesh Kumar, P.M., Gangwar, S.K., Elangovan, C., Maji, M.D. and Saratchandra, B. (1999). Crop loss assessment due to powdery mildew in mulberry. Bull. Seric. Res., 9 : 31-35.

  • Ryu, J., Madhaiyan, M., Poonguzhali, S., Yim, W., Indiragandhi, P., Kim, K., Anandham, R., Yun, J. and Sa, T. (2006). Plant growth substances produced by

  • Methylobacterium spp. and their effect on tomato (Lycopersicon esculentum L.) and red pepper (Capsicum annuum L.) growth. J. Microbiol. Biotechnol., 16 : 1622–1628.

  • Saikaia, P. and Chowdhury, H.D. (1993). Phylloplane microflora for the control of bacterial leaf blight of rice caused by Xanthomonas oryzae pv. oryzae. Indian Phytopath., 46 : 218 - 223.

  • Sandhya, Y., Rajan, C.P.D. and Reddikumar, M. (2018). Antagonistic Effect of Pseudomonas fluorescens on the mycelial growth and the viability of the sclerotia of Rhizoctonia solani in vitro and in soil. Int. J. Curr. Microbiol. App. Sci., 6 : 3038-3045.

  • Sowndhararajan, K., Marimuthu, S., Manian, S. (2012). Biocontrol potential of phylloplane bacteriumOchrobactrum anthropi BMO-111 against blister blight disease of tea. J. Appl. Microbiol., 114 : 209-218.

  • Vorholt, J.A. (2012). Microbial life in the phyllosphere. Nat. Rev Microbiol., 10 : 828–840.


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  • Growth Promotion and Mildew Suppressive Effect of Phylloplane Bacteria of Mulberry (Morus Spp.)

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Authors

P. M. Pratheesh Kumar
Central Sericultural Research and Training Institute, Srirampuram, Mysuru (Karnataka), India

Abstract


Studies were conducted to evaluate growth promotion and mildew (Phyllactinia corylea) suppressive effect of phylloplane bacteria of mulberry (Morus spp.) in vitro and in vivo and the effective bacteria were identified. Among 18 bacteria, 4 isolates showed highly significant (P<0.01) reduction with >70 per cent suppression of conidial germination. The highest reduction was showed by the isolate Pb-5 (13.87%) by suppressing 86.13 per cent conidial germination followed by Pb-4 (17.23%) with 82.17 per cent. Similarly, six isolates significantly increased the seed germination. Significantly high (P<0.01) seed germination was obtained (93.44%) with treatment of the isolate Pb- 6 followed by Pb-3 (86.22%), Pb-7 (86.22%) and Pb-4 (85.47%).Most effective bacterial isolates were identified as Bacillus megaterium (Pb-1) Bacillus subtilis (Pb-1) Bacillus cereus (Pb-1) and Pseudomonas aeruginosae (Pb-1). In vivo studies revealed highly significant (P<0.01) with >60% reduction of disease severity with the application of B.megaterium (63.42%) and B.cereus (60.73%). The study suggests exploration of either of these bacteria for biological control of mildew in mulberry.

Keywords


Biological Control, Mulberry, Phylloplane Bacteria, Powdery Mildew.

References