International Journal of Plant Protection

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Shelf-Life Assessment of Wettable Powder (WP) Formulations of the Entomopathogenic Fungi Nomuraea rileyi (Farlow) Samson


Affiliations
1 Agricultural Research Station, Niphad, Nasik (M.S.), India
2 Department of Entomology, AICRP on Soybean,Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India
     

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Studies on the effect of storage on viability of developed Nomuraea rileyi (Farlow) Samson 5 % WP formulation A(N30S1/1) and B(N30T1/2G2/1H1/1) (comprising adjuvants, fungus and kaolinite) and N.rileyi alone in kaolinite (control) on viability of the fungus are undertaken. At 10 DAI, surface coverage varied from 100 to 36.67, 100 to 33.33 and 100 to 0.0 per cent in formulation A, B and control, respectively from 0 to 300 days storage samples. The formulations A and B stored upto 150 days showed cent per cent surface coverage against the 71.70 per cent in control.Cfu count varied from 24.33 to 1.33x108, 23.67 to 1.33x108 and 23.33 to 0x108cfu/ml in formulation A, B and control, respectively from 0 to 300 days storage. Formulation A and B maintained their superiority over the control viability of the inoculums, while formulation without adjuvants recorded decline in viability. The reduction in cfu was rapid from 270 to 300 days. Considering surface coverage (%), biomass produced and viability (cfu/g) the N.rileyi 5%WP formulation A, B and control could be stored upto 10, 10 and 7 months, respectively for maximum cfu count of 1x108/g for WP formulations as per norms.

Keywords

Nomuraea rileyi, Adjuvants, Glycerol, Sunflower Oil, Tween 80, Honey, Colony Forming Unit.
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  • Alves, R.T., Oliveira, M.A.S., Batenan, R.P., Prior, C. and Leather, S.R. (2001). Spreading and effectiveness of an emulsifiable adjuvant oil fungal formulation. Bulletin de Pesquisa-e Desenvolvimento Embrapa Cerrados, 6: 14.

  • Anonymous (2003). Biopesticide, Agrolook, 4(1) : 4-5.

  • Chaudhari, C.S. (2010). Isolation, production and evaluation of Nomuraea rileyi (Farlow) Samson against Spodopteralitura (Fabricius). Ph. D. Thesis, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmed Nagar, M.S.(India).

  • Faria, M.R., Martins, I., Mello, R., Tigano, M.S. and Faria, M.R. (1999). Entomopathogenic fungal (hyphomycetes) collection: assessment of conidial viability. Colecao de fungos entomopatogenicos (Hyphomycetes): avaliacao da viabilidade conidial. Pesquisa Agropecuaria Brasileira, 34 (8) : 1497-1503.

  • Hajek, A.E. and Lager, R.J. (1994). Interactions between fungal pathogen and insect hosts. Annu.Rev. Entomol., 39: 293-322.

  • Hall, I.M. and Bell, I.V. (1961). Further studies on the effect of temperature on the growth of some entomophthoraceous fungi. J. Insect Pathol., 3: 289-296.

  • Hidalgo, E., Moore, D. and Patourel, G.L. (1998). The effect of different formulations of Beauveria bassiana on Sitophilus zeamaisin stored maize. J. Stored Prod. Res., 34 (2-3) : 171-179.

  • Lingappa, S. and Patil, R.K. (2002). Nomuraea rileyi –A Potential Mycoinsecticide.University of Agricultural Sciences, Dharwad, 30 pp.

  • Ming Guang Feng, L.R., Johnso, J.B. and Kish, L.P. (1990). The virulence of Verticillium lecanii and an aphid derived isolate of Beauveria bassiana for six species of cereal infecting aphids. J. Environ. Entom., 19 (3): 815-820.

  • Nahar, P., Yadav, P., Kulye, M., Hadapad, A., Hassani, M., Tuor, U., Keller, S.,Chandele, A.G., Thomas, B. and Deshpande, M.V. (2004). Evaluation of indigenous fungal isolates, MetarhiziumanisopliaeM 34412, Beauveria bassiana B3301 and Nomuraea rileyi N 812 for the control of Helicover paarmigera (Hub.) in pigeonpea field. J. Biological Control, 18 (1) : 1-8.

  • Pandey, K.A. and Kanujia, K. (2005). Effect of different grain media on sporulation, germination and virulence of Beauveria bassiana (Balsamo) Vuillemin against Spodoptera (Fab.) larvae. J. Biological Control, 19 : 129-133.

  • Ramegowda, G.K. (2005). Aerobiology, Epizootiology and Utilization of Nomuraea rileyi (Farlow) Samson. Ph. D. Thesis, University of Agricultural Sciences, Dharwad, Karnataka (India).

  • Shabana,YM., Charudattan, R., Devalerio, J.T. and Elawakil, M.A. (1977). An evaluation of Hydrophilic polimars for formulating the bioherbicide agents Aternaria cassia and A. eichhorniae. Weed Technology, 11 : 212-220.

  • Silva, L.D.A., Silva, R.F.P., Heineck, M.A. and Silva, L. (1993). Parasites, predators anddiseases of larvae of Diacrisia oblique (Lepidoptera: Arctidae) on soybean, Current Sci., 44: 481-482.

  • Vimladevi, P.S.V., Anitha Chowdary, Prasad, Y.G. and Chowdary, A. (2002). Compatibility of entomopathogenic fungus Nomuraea rileyi with commonly used pesticides. Indian J. Agric. Sci., 72 (6) : 370-372.


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  • Shelf-Life Assessment of Wettable Powder (WP) Formulations of the Entomopathogenic Fungi Nomuraea rileyi (Farlow) Samson

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Authors

S. D. Patil
Agricultural Research Station, Niphad, Nasik (M.S.), India
R. S. Jadhav
Department of Entomology, AICRP on Soybean,Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India

Abstract


Studies on the effect of storage on viability of developed Nomuraea rileyi (Farlow) Samson 5 % WP formulation A(N30S1/1) and B(N30T1/2G2/1H1/1) (comprising adjuvants, fungus and kaolinite) and N.rileyi alone in kaolinite (control) on viability of the fungus are undertaken. At 10 DAI, surface coverage varied from 100 to 36.67, 100 to 33.33 and 100 to 0.0 per cent in formulation A, B and control, respectively from 0 to 300 days storage samples. The formulations A and B stored upto 150 days showed cent per cent surface coverage against the 71.70 per cent in control.Cfu count varied from 24.33 to 1.33x108, 23.67 to 1.33x108 and 23.33 to 0x108cfu/ml in formulation A, B and control, respectively from 0 to 300 days storage. Formulation A and B maintained their superiority over the control viability of the inoculums, while formulation without adjuvants recorded decline in viability. The reduction in cfu was rapid from 270 to 300 days. Considering surface coverage (%), biomass produced and viability (cfu/g) the N.rileyi 5%WP formulation A, B and control could be stored upto 10, 10 and 7 months, respectively for maximum cfu count of 1x108/g for WP formulations as per norms.

Keywords


Nomuraea rileyi, Adjuvants, Glycerol, Sunflower Oil, Tween 80, Honey, Colony Forming Unit.

References