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In vitro Antagonistic Activity of Trichoderma Species against Important Soil Borne Pathogens
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The rhizospheric soil samples were collected from different cultivated agricultural fields from Bharuch and Narmada districts and the mycoflora were isolated by serial dilution plate technique. Total eight isolates of Trichoderma viride, Trichoderma harzianum and Trichoderma longibrachiatum were isolated on Potato Dextrose Agar medium. The green coloured colonies were identified by comparing with taxonomic key. They were purified by single spore isolation method and maintained on PDA slants at 40C in the refrigerator at Department of Pl.Pathology, NMCA, NAU, Navsari. Soil borne Pathogenic fungi viz., Sclerotium rolfsii, Macrophomina phaseolina and Fusarium oxysporum were isolated from the respective diseased plants during field survey in Navsari Agricultural University farm, Navsari. The antagonistic efficacy against test pathogen was evaluated by dual culture plate technique. Among all 8 Trichoderma isolates, The Trichoderma harzianum NCJD8 isolate has showed 24.17 mm mycelial growth with 73.15 per cent inhibition of Sclerotium rolfsii, where in case of Macrophomina phaseolina, Minimum mycelial growth (32.67 mm) of test pathogen was recorded in T. longibracheatum NCJD2 isolate with 63.70 per cent inhibition which was statistically at par with T. viride NCJD6 (34.50 mm) with 61.67 per cent inhibition and when it comes to Fusarium oxysporum, T. harzianum NCJD5 showed minimum mycelial growth and highest per cent growth inhibition (75.56%) with 22.00 mm colony diameter of the pathogen after seven days of incubation which was statistically at par with isolate T. harzianum NCJD1 (72.96%) with 24.33 mm colony diameter.
Keywords
Antagonistic Activity, Soil Borne Pathogens.
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- Aneja, K.R. (2003). Experiments in Microbiology, Plant Pathology and Biotechnology, 4th ed., New Age International (P) Ltd.
- Dennis, C. and Webster, J. (1971a). Antagonistic properties of species- groups of Trichoderma II. Production of volatile antibiotics. Transactions British Mycological Society, 57 : 41–43.
- Dennis, C. and Webster, J. (1971b). Antagonistic properties of species groups of Trichoderma I. Production of non-volatile antibiotics. Transactions British Mycological Society, 57 : 25–39.
- Edgington, L.V., Khew, K.L. and Barrron, G.L. (1971). Fungitoxic Spectrum of Benzimidazole Compounds. Phytopathol., 61 : 42 - 44.
- Gaigole, A.H., Wagh, G.N. and Khadse, A.C. (2011). Antifungal activity of Trichoderma spesies against soil borne pathogen. Asiatic J. Biotechnol. Resourc., 4 : 461-465.
- Gurha, S.N. (2001). Effect of some Trichoderma spp. on the growth of different isolates of Fusarium oxysporum f.sp. ciceri in vitro. Annals Plant Protec. Sci., 9(2) : 332-334.
- Johnson, L.F. and Curl, E.A. (1972).Methods for research on the ecology of soil borne plant pathogens, Burgress Publishing Co, Minneapolis, pp.v+247.
- Madhusudan, P., Gopal, K., Haritha, V., Sangale, U.R. and Rao, S.V.R.K. (2010). Compatability of Trichoderma viride with fungicides and efficiency against Fusarium solani. J. Plant Dis. Sci., 5 : 23-26.
- Pan, S. and Bhagat, S. (2007). Antagonistic potential of Trichoderma and Gliocladium spp. from West Bengal. J. Mycology & Plant Pathol., 37(2) : 235-243.
- Vishwanath, K., Gopal, K. and Gopi, V. (2008). Isolation of potential Trichoderma spp. Associated with dry ischolar_main rot infected acid lime (Citrus aurantifolia Swingle). J. Plant Disease Sci., 3 (2) : 165-168.
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