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Standardization of Culture Age, Crop Growth Stages and Different Methods of Inoculation of Xanthomonas oryzae Pv. Oryzae, A Cause Bacterial Leaf Blight in Rice (Oryza sativa L.)
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Bacterial leaf blight caused by Xanthomonas oryzae pv.oryzae is one of the most dreaded diseases of rice across the world, particularly affecting the majority of rice growing regions of Asia. In India, rice crop is severely affected by BLB and accounts for 6- 74 per cent of estimated yield loss. Since selection of efficient screening method is important for identification of resistance against BLB, so, three investigations were conducted to find out the most efficient Xooculture age, crop growth stage and inoculation method to evolve an efficient and reliable methodology of screening of rice genotypes for identification of resistance against BLB. In the investigations, the rice TN-1 was used as test variety and it maintained as one hill per pot. To find out the appropriate age of Xooculture for its successful inoculation in the host, the plants were inoculated with different ages of Xoocultures at maximum tillering stage. The most effective inoculum age observed for BLB inoculation was 36 hours and 48 hours old culture of Xoo, both in terms of disease severity and incubation period. In case of appropriate crop growth stage for successful inoculation of Xoo, the most susceptible crop growth stage was found to be booting stage followed by panicle initiation stage. Among the different methods of inoculation; the most efficient method was clip + dip followed by clip method.
Keywords
Bacterial Leaf Blight, Culture Age, Rice, TN-1. Xanthomonas oryzae pv. oryzae.
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- Adhikari, T.B., Vera Cruz, C.M., Zhang, Q., Nelson, R.J., Skinner, D.Z., Mew, T.W. and Leach, J.E. (1995).Genetic diversity of Xanthomonas oryzae pv.oryzaein Asia. Appl. & Environ. Microbiol., 61 (3):966-971.
- Anonymous (2016). Annual Report. Department of Agriculture, Cooperation & Farmers Welfare, Ministry of Agriculture & Farmers Welfare, Government of India, Krishi Bhawan, New Delhi-110 001.
- Chahal, S.S. (2005).Disease scenario in rice during post-green revolution era in Punjab. Pl. Dis. Res., 20 : 1-11.
- Chauhan, H.L. (1973).Studies on bacterial leaf blight of paddy (Oryza sativaL.) caused by Xanthomonas oryzae(Uyeda and Ishiyama) Dowson. M.Sc. (Ag.) Thesis, Gujarat Agricultural University, S.K. Nagar.
- Das, L. and Rabindran, R. (2012).Molecular variability in Xanthomonas oryzae pv.oryzaeisolates of Tamil-Nadu. Indian J. Plant Protec., 40(2):123-128.
- Gnanamanickam, S.S., Priyadarsini, V.B., Narayanan, N.N., Vasudevan, P. and Kavitha, S. (1999).An overview of bacterial blight disease of rice and strategies for its management. Curr.Sci., 77(11):1435-1443.
- Gupta, A.K., Sharma, S.C. and Saini, R.G. (1986).Variation in pathogenicity of some Indian isolates of X. oryzae pv. oryzae. Phytopathology, 76 (9):881-883.
- IRRI (1996). Standard Evaluation System Manual. International Rice Research Institute, Manila, Philippines. p. 35.
- Kauffman, H.E., Reddy, A.P.K., Hsieh, S.P.Y. and Nerca, S.D. (1973).An improved technique for evaluating resistance of rice varieties to Xantomonas oryzae. Plant Disease Reporter, 57 : 537-541.
- Khatkar, B.S., Chaudhary, N. and Dangi, P. (2016).Production and Consumption of Grains: India. In: Wrigley, C., Corke, H., and Seetharaman, K., Faubion, J., (eds.) Encyclopedia of Food Grains, 2nd Ed., pp. 367-373. Oxford: Academic Press.
- Koch, M.F. and Mew, T.W. (1991).Effect of plant age and leaf maturity on the quantitative resistance of rice cultivars to X. c. pv. oryzae. Plant Disease, 75(9):901-904.
- Mahmood, M. and Singh, M.N. (1970).Investigations on bacterial blight of paddy caused by Xanthomonas oryzae (Uyeda and Ishiyama) Dowson. Science & Culture, 36(3):170-171.
- Mary, C.A., Nair, S.K. and Saraswathy, P. (2001 b).Efficacy of prophylactic and curative sprayings on the control of bacterial blight of rice. Tropical Agric., 39(1):73-75.
- Mew, T.W., Alwarez, A.M., Leach, J.E. and Swings, J. (1993). Focus on bacterial blight of rice. Plant Disease, 77(1) : 5-12.
- Mew, T.W. (1992).Bacterial blight. In: Compendium of rice diseases. Webster, R.K. and Pamela, S.G. eds. St. Paul: Amer. Phytopathol. Soc., pp. 10-11.
- Patel, S.J. (2008).Management of bacterial blight of rice. Ph. D. (Ag.) Thesis. Dept. of Plant Pathology, N. M. College of Agriculture, Navsari Agricultural University, Navsari – 396 450, Gujarat, 302 pp.
- Prasad, D. Singh, R. and Deep, S. (2018). In-vitro and In-vivo Efficacy of Antibacterial Compounds against Xanthomonas oryzae pv. oryzae, A Cause of Bacterial Leaf Blight of Rice. Internat. J. Curr. Microbiol. App. Sci., 7(05): 2960-2969.
- Raju, R.A. (2000). Glimpses of Rice Technology. Agro Bios., New Delhi.
- Singh, R.S. (2009). Plant Diseases. Oxford & IBH. Pub. Co. Pvt. Ltd. pp.64-65.
- Srivastava, D.N. (1967).Epidemiology and control of BLB of rice in India. In: proceedings of asymposium in rice diseases and their control by growing resistant varieties and other measures. Japan Agriculture, Forestry and Fisheries Research Council, 11:18.
- Sukhatme, P.V. and Amble, V.N. (1985). Statistical Methods for Agricultural Workers, Publication and Information Division, New Delhi, pp. 553.
- Suryadi, Y. (1990).Development of kresek symptoms on some rice varieties. Internat. Rice Res. Notes, 15(3):13.
- USDA (2017). World Agricultural Production, United States Department of Agriculture, Foreign Agricultural Service/Office of Global Analysis, International Production Assessment Division (IPAD). Circular Series WAP 08-17.
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