Characterization of pseudomonas Savastanoi Pv. Glycinea, the Cause of Bacterial Blight in Soybean

Pseudomonas savastanoi pv. glycinea isolated from tan coloured spots on the cotyledons of soybean was gram negative, short rod , 0.6-0.8 ×1.2-30 μ, non spore former and motile by a single polar flagellum. King's B medium was best supporting for the growth of bacterium. Growth of the bacterium was maximum at temperature 25ºC and pH 6.8. The pathogen was unable to liquify gelatin and produced acid from glucose and sucrose but not from starch and was not able to produce gas from any of the carbon sources tested and also failed to hydrolyse starch and H2S production. Growth of the bacterial isolate was maximum in King's B medium supplemented with arabinose, ammonium sulphate and tryptophan.