Evaluation of Bio-agents and Antibiotics against xanthomonas Axonopodis Pv. Punicae, Causal Agent of Bacterial Blight of Pomegranate

In the present studies the antagonistic microorganisms and antibiotics were tested for their efficiency in inhibition of growth of Xanthomonas axonopodis pv. punicae, the incetant of bacterial blight of pomegranate. Among the different isolates of bioagents, Pseudomonas florescens (Pf4) was found significantly superior in inhibiting (15.73 mm) the growth of X. a. pv. punicae but remained on par with Bacillus subtilis isolates BS1 and BS2 with 12.1 and 13.66 mm, respectively. Whereas bioagents BS3 and Pf6 showed lower inhibitory zone of 0.76 and0.93 mm, respectively. The isolates of bioagents BS 4, Tv-16, Tv-R, Th-10 and Th-R recorded zero inhibition zone. Differences among the treatments and concentrations were found to be statistically significant except between streptocycline and streptocycline + CuSO4. Among them, streptocycline was found to be the best and was significantly superior from the rest of the chemicals with mean inhibition zone of 25.75 mm in all the tested concentrations, followed by streptocycline + CuSO4 (20.24) and bactinash-200 (11.74). Among different concentrations, 1000 ppm recorded the mean maximum inhibition zone (11.33 mm) followed by 750 and 500 ppm. Whereas the least inhibition was observed at 250 ppm (8.33 mm).