International Journal of Plant Protection

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Presence of Phytoplasma Infections in Papaya (carica Papaya L.) Plants in Uttar Pradesh, India


Affiliations
1 Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
2 College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), India
     

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During survey of papaya fields in Meerut, severe symptoms of leaf yellowing, intervenial chlorosis, and curl apical necrosis were observed in approximately 25 per cent of plants in each of the papaya fields. DNA extracted from leaf, midrib and bark of symptomatic and healthy plants of papaya were preceded with universal primer pairs. Expected ~1600 bp fragments were amplified with primer pair P1/Tint in diseased plants. Infection of phytoplasma disease in papaya plants was confirmed by the PCR.

Keywords

Papaya, Phytoplasma, Curl Apical Necrosis, Pcr
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  • Presence of Phytoplasma Infections in Papaya (carica Papaya L.) Plants in Uttar Pradesh, India

Abstract Views: 441  |  PDF Views: 0

Authors

Anchal Rani
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Pragati Misra
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Jitendra Singh
College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), India
Pankaj Kumar
College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), India
Rosy Rani
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Pradeep Shukla
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India

Abstract


During survey of papaya fields in Meerut, severe symptoms of leaf yellowing, intervenial chlorosis, and curl apical necrosis were observed in approximately 25 per cent of plants in each of the papaya fields. DNA extracted from leaf, midrib and bark of symptomatic and healthy plants of papaya were preceded with universal primer pairs. Expected ~1600 bp fragments were amplified with primer pair P1/Tint in diseased plants. Infection of phytoplasma disease in papaya plants was confirmed by the PCR.

Keywords


Papaya, Phytoplasma, Curl Apical Necrosis, Pcr