In Vitro Efficacy of Different Botanicals, Bioagents, Chemicals against Xanthomonas axonopodis Pv.citri by Turbidiometrical Method

Citrus canker disease of acid lime caused by Xanthomonas axonopodis pv. citri is an important disease in many parts of MH region. The bacterium infects the twigs, petioles, fruit stalks and fruits. Action of botanicals, bioagents,chemicals against Xac was studied by turbidiometrically at 24hrs.intervals upto 96hrs by spectrophotometer at 620nm. At 96 hours of incubation least bacterial growth(0.232OD) was exhibited in copperoxychloride + streptomycin sulphate (0.2 % + 200ppm) followed with copper-oxychloride + streptomycin sulphate (0.2% + 100ppm) 0.266 OD statistically superior over all treatments. Similar findings are observed after 24h growth in copper oxychloride + streptomycin sulphate (0.2 % + 200ppm) followed with copper-oxychloride +streptomycin sulphate (0.2 % + 100ppm) (0.303, 0.306, respectively. In botanicals and bioagents neem seed kernel extract (5%) was effective in reducing the growth of bacteria with 0.446 OD at 96 h followed by Pseudomonas flourescence 1×10⁸ cell and Bacillus subtilis 1×10⁸ cell with 0.506 and 0.486 OD, respectively.