International Journal of Plant Sciences

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Efficacy of Pseudomonas fluorescens and Trichoderma viride Based Bioformulation for Management of Bacterial Wilt Disease of Ginger


Affiliations
1 Krishi Vigyan Kendra, Sonitpur (Assam), India
2 Department of Plant Pathology, Assam Agricultural University, Jorhat (ASSAM), India
3 Regional Research Station (A.A.U.), North Lakhimpura (Assam), India
     

   Subscribe/Renew Journal


An experiment was conducted to evaluate the efficacy of a consortia formulation of Pseudomonas fluorescens and Trichoderma harzianum for management of bacterial wilt disease of ginger in Assam. Inhibitory effect of the biocontrol agents was evaluated in vitro following dual culture assay method for their efficacy against Ralstonia solanacearum, the ginger wilt pathogen. Quantitative assay of population dynamics of the two antagonists, mass cultured in organic substrates viz., vermicompost (VC) and mustard oil cake (MOC) revealed that the antagonists maintained a high population count up to 120 days of storage at room temperature. Pseudomonas fluorescens recorded highest average population (45.47 x 107cfu/g) when mass cultured in the mixture of VC and MOC, while T. harzianum recorded maximum average population (34.14 x 107cfu/g) when mass cultured in MOC. Bioformulations were further evaluated for their efficacy in ginger wilt management under field condition. Efficacy of one fungicide (Copper oxychloride) and an antibiotic (Streptocycline) was also tested for comparison. Lowest disease incidence (15.63%) was recorded in the treatment of seed treatment (ST) and soil application (SA) of P. fluorescens and T. harzianum consortia mass cultured in the mixture of VC and MOC. It was followed by ST and SA of T. harzianum mass cultured in MOC (21.88%), which was statistically at par with the application of copper oxychloride (26.25%).

Keywords

Antagonists, Bacterial Wilt, Biological Management, Ginger, Substrates.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Alexander, M. (1997). Introduction to soil microbiology. John Wiley and Sons. New York, 234 pp.

  • Ali, S.A., Saraf, R.K. and Pathak, R.K. (1995). Varietal performance of ginger against rhizome rot. Plant Dis. Res., 10(2) : 153 -155.

  • Anonymous (2005). ‘Karbi-Anglong’ the highest ginger producing district of India, Dainik Janambhumi, Nov. 16, 2005, Published from Jorhat and Guwahati, ASSAM (INDIA).

  • Aspiras, R.B. and Dela Cruz, A.R. (1985). Potential biological control of bacterial wilt in tomato and potato with Bacillus polymyxa Fu 6 and Pseudomonas fluorescens. In : Bacterial wilt disease in Asia and the South Pacific. GD Persely (Ed). ACIAR ProceedingsNo. 13: 89-92.

  • Ayer, W.A. and Adam, P.B. (1981). Mycoparasitism and its application to biological control of plant diseases. In : Biological control in crop production. Papavizas, G.C. (ed.), BARC Symposium, pp. 91-103.

  • Bora, L.C. and Bora, Popy (2008). Biological control strategies for management of bacterial wilt of brinjal (Solanum melongena L.). J. Mycol. Pl. Pathol., 38(3) : 542-545.

  • Bora, L.C., Das, Minku and Das, B.C. (2000). Influence of microbial antagonists and soil amendments on bacterial wilt severity and yield of tomato. (Lycopersicon esculentum). Indian J. Agric. Sci., 70(6) : 390-392.

  • Bora, Popy, Bora, L.C., Deka, P.C. and Begum, M.(2013). Ecofriendly management of bacterial wilt disease in brinjal through application of antagonistic microbial population. J. Biolog. Control, 27(1) : 29-34.

  • Ciampi-Panno, P.L., Fuentes, P.R., Schebitz, T.R. and Ortega, A.S. (1996). Biological control of Pseudomonas solanacearum, bacterial wilt agent. I. Growth of Pseudomonas fluorescens strain BC 8. Agro-Sur., 24: 32-38.

  • Compant, D., Duffy, B., Nowak, J., Clément, C. and Barka, E.A. (2005). Use of plant growth-promoting bacteria for biocontrol of plant diseases: Principles, mechanisms of action, and future prospects. Appl. Eviron. Microbiol., 71 : 4951-4959.

  • Danielson, R.M. and Davey, C.B. (1973). Non-nutritional factors affecting the growth of Trichoderma in culture. Soil Biol. Biochem., 5 : 495-504.

  • Dennis, C. and Webster, J. (1971). Antagonistic properties of species of Trichoderma. 1 production of non -volatile antibiotics. Trans. Brit. Mycol. Soc., 57 : 25-39.

  • Dev Nath, H., Pathak, J.J. and Bora, L.C. (2001). Studies on bacterial wilt of ginger caused by Ralstonia solanacearum Yabuuchi et al. in Assam. J. Agric. Sci. Soc., (JASS) 14(2) : 155-158.

  • Fisher, R.A. (1937). Statistical methods for research worker. Oliver and Boyd, Edinburg.

  • Hoitink, H.A.J. and Fahy, P.C. (1986). Basis for the control of soil borne plant pathogens with compost. Ann. Rev. Phytopath., 24 : 93-144.

  • Huber, D.M. and Watson, R.D. (1970). Effect of organic amendment on soil borne pathogens. Phytopathol., 66 : 22-26.

  • Karuna, K. and Khan, A.N.A. (1994). Biological control of wilt of tomato caused by Pseudomonas solanacearum using antagonistic bacteria. Indian Phytopath., 47 : 326.

  • Loper, J.E. and Buyer, J.S. (1991). Siderophores on microbial interaction on plant surfaces. Mol. Plant Microbe. Interact., 4 : 5-15.

  • Meena, B., Radhajeyalakshmi, R., Marimuthu, T., Vidhyasekaran, P. and Velazhahan, R. (2002). Biological control of groundnut late leaf spot and rust by seed and foliar applications of a powder formulation of Pseudomonas fluorescens. Biocontrol Sci. Technol., 12 : 195-204.

  • Nautyal, C.S. (2000). In : Biocontrol potential and its exploitation in sustainable agriculture, Edited By R. K. Upadhyay, K. G. Mukerji And B. P. Chamola. Kluwer Academic/Plenum Publishers, New York. pp 9-23.

  • Papavizas, G.C. (1985). Trichoderma andGliocladium : Biology, ecology and potential for biocontrol. Ann. Rev. Phytopath., 23 : 23-54.

  • Papavizas, G.C. and Lumsden, R.D. (1980). Biological control of soil borne fungal propagules. Ann. Rev. Phytopath., 18 : 389-413.

  • Radhakrishnan, N.N. and Narayanswamy, R. (1994). Incidences of dry ischolar_main rot disease of groundnut as influenced by the application of potassium and certain organic amendments. In : Crop diseases K Sivaprakasam and K Seetharaman (Eds) pp. 227-231.

  • Rahman, H., Karuppaiyan, R., Kishore, K. and Denzongpa, R. (2009). Traditional practices of ginger in Northeast India. Indian J. Trad. Knowl., 8(1) : 23-28.

  • Sharif, T., Khalil, S. and Ahmad, S. (2003). Effect of Rhizobium sp., on growth of pathogenic fungi under in vitro conditions. Pakistan J. Biological Sci., 6: 1597–1599.

  • Suslow, T.V. and Schroth, M.N. (1982). Rhizobacteria of sugarbeets: effects of seed application and ischolar_main colonization on yield. Phytopathol., 72 : 199-206.

  • Ushasree, N.N., Chandrasekharan, S. and Govindasamy, R. (1989). Humic acid and its influence on crop plants. National Seminar on Humic Acid in Agriculture. Annamalai University, Tamil Nadu. 63 p. (Abs).

  • Vidhyasekaran, P. and Muthamilan, M. (1995). Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis., 79 : 782-786.

  • Waksman, S. (1922). A method of counting the number of fungi in soil. J. Bacteriol., 7 : 339-341.


Abstract Views: 371

PDF Views: 0




  • Efficacy of Pseudomonas fluorescens and Trichoderma viride Based Bioformulation for Management of Bacterial Wilt Disease of Ginger

Abstract Views: 371  |  PDF Views: 0

Authors

Popy Bora
Krishi Vigyan Kendra, Sonitpur (Assam), India
L. C. Bora
Department of Plant Pathology, Assam Agricultural University, Jorhat (ASSAM), India
P. C. Deka
Krishi Vigyan Kendra, Sonitpur (Assam), India
Bikram Borkotoki
Regional Research Station (A.A.U.), North Lakhimpura (Assam), India
A. K. Sharma
Krishi Vigyan Kendra, Sonitpur (Assam), India
H. S. Dutta
Krishi Vigyan Kendra, Sonitpur (Assam), India
Debahaj Buhagohain
Krishi Vigyan Kendra, Sonitpur (Assam), India

Abstract


An experiment was conducted to evaluate the efficacy of a consortia formulation of Pseudomonas fluorescens and Trichoderma harzianum for management of bacterial wilt disease of ginger in Assam. Inhibitory effect of the biocontrol agents was evaluated in vitro following dual culture assay method for their efficacy against Ralstonia solanacearum, the ginger wilt pathogen. Quantitative assay of population dynamics of the two antagonists, mass cultured in organic substrates viz., vermicompost (VC) and mustard oil cake (MOC) revealed that the antagonists maintained a high population count up to 120 days of storage at room temperature. Pseudomonas fluorescens recorded highest average population (45.47 x 107cfu/g) when mass cultured in the mixture of VC and MOC, while T. harzianum recorded maximum average population (34.14 x 107cfu/g) when mass cultured in MOC. Bioformulations were further evaluated for their efficacy in ginger wilt management under field condition. Efficacy of one fungicide (Copper oxychloride) and an antibiotic (Streptocycline) was also tested for comparison. Lowest disease incidence (15.63%) was recorded in the treatment of seed treatment (ST) and soil application (SA) of P. fluorescens and T. harzianum consortia mass cultured in the mixture of VC and MOC. It was followed by ST and SA of T. harzianum mass cultured in MOC (21.88%), which was statistically at par with the application of copper oxychloride (26.25%).

Keywords


Antagonists, Bacterial Wilt, Biological Management, Ginger, Substrates.

References