International Journal of Plant Sciences

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

In Vitro Study on Salinity Screening in Rice


Affiliations
1 Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Killikulam, Vallanad (T. N.), India
     

   Subscribe/Renew Journal


Two moderately resistant rice cultivars, White ponni and BPT - 52904 were used to selection of promising saline tolerant callus lines through screen for salinity tolerance under in vitro condition. Embryogenic calli was obtained when tissue culture basal media supplemented with 2 mg L-1 2,4-D along with 0.5 mg L-1 kinetin and 1 mg L-1 NAA. A significant reduction in callus growth rate (Relative growth rate) and callus induction per cent was noticed in media supplemented with different concentration of saline treatment, from 0.5 to 1.0 and 1.5 per cent NaCl. But the time elapsed for callus induction was increased with higher concentration of salt in the medium. A significant number embrogenic calli was survived in lower concentration, 0.5 and 1.0 per cent of NaCl showed good regeneration capacity in regeneration media. Few promising saline tolerant plants were recovered and transferred to the ischolar_maining media before adapting to acclimatization. Hence, this in vitro technique with different NaCl stress could have been used effectively to screening for salt screening in rice, rather than the field screening.

Keywords

Relative Growth Rate, Embryogenic Calli, Regeneration, Plantlets, Salinity Screening.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Aditya, T.L. and Backer, B.A. (2006). Selection of salt tolerant somaclones from indica rice through continuous in vitro and ex vitro sodium chloride stress. Indian. J. Pl. Physiol., 11 : 349-357.

  • Ahmad, M.S.A., Javed, F. and Ashraf, M. (2007). Iso-osmotic effect of NaCl and PEG on growth, cations and free proline accumulation in callus tissue of two indica rice (Oryza sativa L.) genotypes. Pl. Growth Regul., 53 : 53-63.

  • Al-Forkan, M., Rahim, M.A., Chowdhury, T., Akter, P. and Khaleda, L. (2005). Development of highly callogenesis and regeneration system for some tolerant rice (Oryza sativa L.) cultivars of Bangladesh. Biotechnol., 4 : 230-234.

  • Attia, S.A.A., Nadia Gad., Abdel-Rahman, H.M., Shenoda, J.E. and Aida, A. Rizkalla (2014). In vitro enhancement of salinity tolerance in rice using cobalt sulfate. World Appl. Sci. J., 31(7) : 1311-1320.

  • Barakat, M.N. and Abdel-Latif, T.H. (1996). In vitro selection of wheat callus tolerant to high levels of salt and plant regeneration. Euphytica, 91 : 127-140.

  • Bashir, M.U., Akbar, N., Iqbal, A. and Zaman, H. (2010). Effect of different sowing dates on yield and yield components of direct seeded coarse rice (Oryza sativa L.). Pakisan J. Agri. Sci., 47 : 361-365.

  • Bohnert, H.J. and Jensen, R.G. (1996). Strategies for engineering water-stress tolerance in plants. Trends Biotechnol., 14 : 89-97.

  • Cheema, A.A., Saleem, M.Y., and Awan, M.A. (2002). In vitro techniques for the selection of Basmati rice mutants better adapted to saline environments. In: Mutation, in vitro and molecular techniques for environmentally sustainable crop improvement. M. Mauszynski and K.J. Kasha (ed.) IAEA, Vienna. 161-168 pp.

  • Flowers, T.J. and Yeo, A.R. (1995). Breeding for salinity resistance in crop plants -Aust. J. Pl. Physiol., 22 : 875-884.

  • Gulati, A. and Jaiwal, P.K. (2010). In citro induction of multiple shoots and regeneration from shoot tips of mung bean (Vigna radiata L. Wilczke). Plant Cell, Tiss. & Org. Cult., 29 : 199-205.

  • Lee, I.S., Kim, D.S., Hyun, D.Y., Lee, S.J., Song, H.S., Lim, Y.P. and Lee, Y.I. (2003). Isolation of gamma-induced rice mutants with increased tolerance to salt by anther culture. J. Pl. Biotechnol., 5(1) : 51-57.

  • Lin, Y.J. and Zhang, Q. (2005). Optimizing the tissue culture conditions for high efficiency transformation of indica rice. Pl. Cell Rep., 23 : 540-547.

  • Lutts, S., Bouharmont, J. and Kinet, J.M. (1999). Physiological characterization of salt resistant rice (Oryza sativa L.) somaclones. Aust. J. Bot., 47 : 835-849.

  • Munns, R., Hare, A., James, R.A. and Rebetzke, G.J. (1999). Genetic variation for improving the salt tolerance of duram wheat. Aust. J. Agril. Res., 51 : 69-74.

  • Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth bioassays with tobacco tissue culture. Pl. Physiol., 15 : 473-497.

  • Pandey, S.K., Ramesh, B. and Gupta, P.K.S. (1994). Callusing and plant regeneration in rice. Indian. J. Genet., 54: 293.

  • Raveendar, S., Premkumar, A., Ignacimuthu, S. and Agastian, A. (2008). Effect of sea water on callus induction and regeneration of rice genotypes. Internat. J. Integrative Biol., 3(2) : 92.

  • Sathish, P., Gamborg, O.L. and Nabors, N. W. (1995). Rice anther culture: callus initiation and somaclonal variation in progeny of regenerated plants. Pl. Cell Rep., 14 : 432-436.

  • Shanthi, P., Jebaraj, S. and Geeth, S. (2010). In vitro screening for salt tolerance in Rice (Oryza sativa). Electronic J. Pl. Breed., 1(4) : 1208-1212.

  • Smith, M.K. and McComb, J.A. (1981b). Effect of NaCl on growth of whole plants and corresponding callus cultures. Austr. J. Pl. Physiol., 8 : 267-275.

  • Zinnah, K.M.A., Nayem Zobayer, Saif, U., Sikdar., Lutfun Nahar Liza., Md. Al Nayem Chowdhury and M. Ashrafuzzaman (2013). In vitro regeneration and screening for salt tolerance in rice (Oryza sativa L.). Internat. Res. J. Biological Sci., 2(11) : 29-36.


Abstract Views: 483

PDF Views: 0




  • In Vitro Study on Salinity Screening in Rice

Abstract Views: 483  |  PDF Views: 0

Authors

G. Thamodharan
Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Killikulam, Vallanad (T. N.), India
M. Arumugam Pillai
Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Killikulam, Vallanad (T. N.), India

Abstract


Two moderately resistant rice cultivars, White ponni and BPT - 52904 were used to selection of promising saline tolerant callus lines through screen for salinity tolerance under in vitro condition. Embryogenic calli was obtained when tissue culture basal media supplemented with 2 mg L-1 2,4-D along with 0.5 mg L-1 kinetin and 1 mg L-1 NAA. A significant reduction in callus growth rate (Relative growth rate) and callus induction per cent was noticed in media supplemented with different concentration of saline treatment, from 0.5 to 1.0 and 1.5 per cent NaCl. But the time elapsed for callus induction was increased with higher concentration of salt in the medium. A significant number embrogenic calli was survived in lower concentration, 0.5 and 1.0 per cent of NaCl showed good regeneration capacity in regeneration media. Few promising saline tolerant plants were recovered and transferred to the ischolar_maining media before adapting to acclimatization. Hence, this in vitro technique with different NaCl stress could have been used effectively to screening for salt screening in rice, rather than the field screening.

Keywords


Relative Growth Rate, Embryogenic Calli, Regeneration, Plantlets, Salinity Screening.

References