International Journal of Plant Sciences

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In Vitro Study on Salinity Screening in Rice


Affiliations
1 Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Killikulam, Vallanad (T. N.), India
     

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Two moderately resistant rice cultivars, White ponni and BPT - 52904 were used to selection of promising saline tolerant callus lines through screen for salinity tolerance under in vitro condition. Embryogenic calli was obtained when tissue culture basal media supplemented with 2 mg L-1 2,4-D along with 0.5 mg L-1 kinetin and 1 mg L-1 NAA. A significant reduction in callus growth rate (Relative growth rate) and callus induction per cent was noticed in media supplemented with different concentration of saline treatment, from 0.5 to 1.0 and 1.5 per cent NaCl. But the time elapsed for callus induction was increased with higher concentration of salt in the medium. A significant number embrogenic calli was survived in lower concentration, 0.5 and 1.0 per cent of NaCl showed good regeneration capacity in regeneration media. Few promising saline tolerant plants were recovered and transferred to the ischolar_maining media before adapting to acclimatization. Hence, this in vitro technique with different NaCl stress could have been used effectively to screening for salt screening in rice, rather than the field screening.

Keywords

Relative Growth Rate, Embryogenic Calli, Regeneration, Plantlets, Salinity Screening.
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  • In Vitro Study on Salinity Screening in Rice

Abstract Views: 476  |  PDF Views: 0

Authors

G. Thamodharan
Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Killikulam, Vallanad (T. N.), India
M. Arumugam Pillai
Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Killikulam, Vallanad (T. N.), India

Abstract


Two moderately resistant rice cultivars, White ponni and BPT - 52904 were used to selection of promising saline tolerant callus lines through screen for salinity tolerance under in vitro condition. Embryogenic calli was obtained when tissue culture basal media supplemented with 2 mg L-1 2,4-D along with 0.5 mg L-1 kinetin and 1 mg L-1 NAA. A significant reduction in callus growth rate (Relative growth rate) and callus induction per cent was noticed in media supplemented with different concentration of saline treatment, from 0.5 to 1.0 and 1.5 per cent NaCl. But the time elapsed for callus induction was increased with higher concentration of salt in the medium. A significant number embrogenic calli was survived in lower concentration, 0.5 and 1.0 per cent of NaCl showed good regeneration capacity in regeneration media. Few promising saline tolerant plants were recovered and transferred to the ischolar_maining media before adapting to acclimatization. Hence, this in vitro technique with different NaCl stress could have been used effectively to screening for salt screening in rice, rather than the field screening.

Keywords


Relative Growth Rate, Embryogenic Calli, Regeneration, Plantlets, Salinity Screening.

References