International Journal of Plant Sciences

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Anti-Microbial Activity of Spices Black Cardamom, Mustard Seed and Liquorice against V. cholera, V. parahaemolyticus and V. vulnificus


Affiliations
1 Department of Microbiology, Singhania University, Jhunjhunu (Rajasthan), India
2 Division of Biotechnology, Cyto Gene Research and Development, Lucknow (U.P.), India
     

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There are several water-borne diseases which are highly pathogenic for humans. Among all, Vibrio genus plays a major role. Vibrio cholerae, a gram negative anaerobe, is one of the most ancient and major water borne pathogen which causes fatal disease, cholera. This study chiefly focuses on the natural or plant derived products which could be used against Vibrio. In this work, 10 pond water samples were collected and investigated for presence or absence of pathogenic Vibrio species. A rapid method, multiplex PCR,of identification has been applied for determining the species. Out of 10, 8 samples showed presence of Vibrio species in them when cultured on selective media. The identified isolates of V. cholera, V. parahaemolyticus and V. vulnificus were then tested against three common spices, black cardamom(Amomum subulatum), mustard seed (Brassica nigra) and liquorice (Glycyrrhiza glabra) , extracts for their antibacterial property using agar well diffusion method. The antimicrobial assay result of spices showed a maximum activity by aqueous extract liquorice (Glycyrrhiza glabra) of 23 ± 0.52 (mm) against V.vulnificus followed by mustard seed (Brassica nigra) of 22.1 ± 0.60 (mm) against V.vulnificus.

Keywords

Black Cardamom, Mustard Seed, Liquorice, mPCR, TOX Gene, Vibrio Species, Antibacterial Activity.
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  • Anti-Microbial Activity of Spices Black Cardamom, Mustard Seed and Liquorice against V. cholera, V. parahaemolyticus and V. vulnificus

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Authors

Naveen Kumar Shukla
Department of Microbiology, Singhania University, Jhunjhunu (Rajasthan), India
Uma Shankar
Division of Biotechnology, Cyto Gene Research and Development, Lucknow (U.P.), India

Abstract


There are several water-borne diseases which are highly pathogenic for humans. Among all, Vibrio genus plays a major role. Vibrio cholerae, a gram negative anaerobe, is one of the most ancient and major water borne pathogen which causes fatal disease, cholera. This study chiefly focuses on the natural or plant derived products which could be used against Vibrio. In this work, 10 pond water samples were collected and investigated for presence or absence of pathogenic Vibrio species. A rapid method, multiplex PCR,of identification has been applied for determining the species. Out of 10, 8 samples showed presence of Vibrio species in them when cultured on selective media. The identified isolates of V. cholera, V. parahaemolyticus and V. vulnificus were then tested against three common spices, black cardamom(Amomum subulatum), mustard seed (Brassica nigra) and liquorice (Glycyrrhiza glabra) , extracts for their antibacterial property using agar well diffusion method. The antimicrobial assay result of spices showed a maximum activity by aqueous extract liquorice (Glycyrrhiza glabra) of 23 ± 0.52 (mm) against V.vulnificus followed by mustard seed (Brassica nigra) of 22.1 ± 0.60 (mm) against V.vulnificus.

Keywords


Black Cardamom, Mustard Seed, Liquorice, mPCR, TOX Gene, Vibrio Species, Antibacterial Activity.

References