International Journal of Plant Sciences

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

In Vitro Screening of Plant Growth Promoting Rhizobacteria to Control Bacterial Wilt (Ralstonia solanacearum) of Tomato (Lycopersicon esculentum)


Affiliations
1 Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
     

   Subscribe/Renew Journal


Ralstonia solanacearum is the causative agent of bacterial wilt that causes considerable damages in the yield of various crop plants. The intent of the study was to evaluate potential of bacterial antagonists to suppress bacterial wilt disease development and evaluate the role of the strains as plant growth-promoting rhizobacteria (PGPR) in tomato. One hundred rhizosphere bacterial isolates were screened against virulent strain of Ralstonia solanacearum. After in vitro screening 10 antagonistic strains designated PR3, PR17, PR26, 1NAB15, 1NAB20, 3NAA1, 3NAA2, 2CBA2, 2CBA4, 2CBA18 showed antagonistic effect by producing inhibition zone supressing the growth of R. Solanacearum. The isolate PR3 showed the highest inhibition zone measuring 33.3mm whereas 1NAB15 showed the lowest zone measuring 10mm. The present study, therefore, suggests that the use of PGPR isolates which showed the antagonistic activity can be used as inoculants/ bioantagonists might be beneficial for the control of bacterial wilt of tomato in field studies.

Keywords

Ralstonia solanacearum, PGPR, Bacterial Wilt, Tomato.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Ali, M.S. (1993). Bacterial wilt of potato in Bangladesh, 196 p. A paper presented on International Workshop on Bacterial wilt of potato held at NEW DELHI, INDIA.

  • Aliye, N., Fininsa, C. and Hiskias, Y. (2008). Evaluation of rhizosphere bacterial antagonists for their potential to bioprotect potato (Solanum tuberosum) against bacterial wilt (Ralstonia solanacearum). Biol. Control, 47: 282- 288.

  • Ciampi-Panno, L., Fernandez, C., Bustamante, P., Andrade, N., Ojeda, S. and Conteras, A. (1989). Biological control of bacterial wilt of potatoes caused by Pseudomonas solanacearum. Am. Potato J., 66 : 315-332.

  • Chakraborty, M., Chattarjee, B., Habib, A.K.M.A., Sengupta, C., Som, M.G. and Samaddar, K.R. (1994). Occurrence of Pseudomonas solanacearumBiovar 3 on eggplant, tomato and potato in West Bengal. ACLAR. Bact. Wilt News, l : 10.

  • Hayward, A.C. (1991). Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol., 29 : 65-87.

  • Hayward, A.C. and Hartman, G.I. (1994). Bacterial wilt, the disese and its causative agent, Psendomonas solanacearum, 259 p. Wallingford.

  • Heil, M. and Bostock, R.M. (2002). Induced systemic resistance (ISR) against pathogens in the context of induced plant defences. Ann. Bot., 89 : 503-512.

  • Kelman, A. (1998). One hundred and one years of research on bacterial wilt. In bacterial wilt disease: molecular and ecological aspects. Prior, P., Allen, C., Elphinstone, J. (Eds)., Springer-Verlag, Berlin Heidelberg.

  • Li, G.C., Jin, L.P., Xie, K.Y. and Qu, D.Y. (2004). Advances in research on bacterial wilt of ginger in China. Chinese Potato, 18 : 350-354.

  • Liu, M., Zhang, M., Ji, J.C., Yin, F.Q., Zhang, Y., Tu, Y. and Ye, Y.J., (2005). Advances in research on bacterial wilt of ginger in China. Chinese Agril. Sci. Bulletin, 21 : 337-341.

  • McLaughlin, R.J., Sequeira, L. and Weingartner, D.P. (1990). Biocontrol of bacterial wilt of potato with an avirulent strain of Pseudomonas solanacearum: interactions with ischolar_main-knot nematodes. American Potato J., 67 (2) : 93-107.

  • Pradhanang, P.M., Ji, P., Momol, M.T., Olson, S.M., Mayfield, J.L. and Jones, J.B. (2005). Application of acibenzolarS-methyl enhances host resistance in tomato against Ralstonia solanacearum. Pl. Dis., 89 : 989-993.


Abstract Views: 507

PDF Views: 0




  • In Vitro Screening of Plant Growth Promoting Rhizobacteria to Control Bacterial Wilt (Ralstonia solanacearum) of Tomato (Lycopersicon esculentum)

Abstract Views: 507  |  PDF Views: 0

Authors

Mehjabeen Afaque
Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Suchit A. John
Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Pradeep K. Shukla
Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Pramod W. Ramteke
Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
K. Prasad Rao
Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India

Abstract


Ralstonia solanacearum is the causative agent of bacterial wilt that causes considerable damages in the yield of various crop plants. The intent of the study was to evaluate potential of bacterial antagonists to suppress bacterial wilt disease development and evaluate the role of the strains as plant growth-promoting rhizobacteria (PGPR) in tomato. One hundred rhizosphere bacterial isolates were screened against virulent strain of Ralstonia solanacearum. After in vitro screening 10 antagonistic strains designated PR3, PR17, PR26, 1NAB15, 1NAB20, 3NAA1, 3NAA2, 2CBA2, 2CBA4, 2CBA18 showed antagonistic effect by producing inhibition zone supressing the growth of R. Solanacearum. The isolate PR3 showed the highest inhibition zone measuring 33.3mm whereas 1NAB15 showed the lowest zone measuring 10mm. The present study, therefore, suggests that the use of PGPR isolates which showed the antagonistic activity can be used as inoculants/ bioantagonists might be beneficial for the control of bacterial wilt of tomato in field studies.

Keywords


Ralstonia solanacearum, PGPR, Bacterial Wilt, Tomato.

References