![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_open_medium.gif)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_restricted_medium.gif)
Liquid Medium Culture Method for Rapid Multiplication of Banana (Musa acuminata) CV. 'Grand Naine' through Tissue Culture
Subscribe/Renew Journal
For an increasing number of plantlets, liquid culture methods have demonstrated a number of important advantages over conventional semi-solid micropropagation, including several fold increase in multiplication rate, reduction in medium cost and also space, energy and lobour requirements. These cost-saving advantages have been the driving force for increased attention to the use of liquid systems. Effects of liquid medium using different simple low cost culture containers on in vitro propagation of banana cv. 'GRAND NAINE' were investigated and compared with conventional solid medium. The treatments studied included: Growtech container with nylon wire mesh support; simple polypropylene container with cotton fibre support; simple polypropylene container with partial immersion, simple polypropylene container with full immersion and conventional baby jar bottle with agar gelled medium. Simple polypropylene container with cotton fibre support was found effective than that of agar-gelled medium. The plantlets produced were also sturdier and better quality.
Keywords
In vitro, Aseptic Cultures, Support Matrix, Microshoots, Vitrification.
Subscription
Login to verify subscription
User
Font Size
Information
- Alvard, D., Cote, F. and Teisson, C. (1993). Comparison of liquid medium culture for banana micropropagation, effects of temporary immersion of explants. Plant Cell Tiss. Org. Cult., 32 : 55-60.
- Besagarhally, R. (1996). Micropropagation and nutritional studies in of tissue cultured banana var. GRAND NAINE. Thesis, Ph.D. University of Agricultural Sciences, Bangalore, KARNATAKA (INDIA).
- Debergh, P.C. (1983). Effects of agar brand and concentration on the tissue culturemedium. Plant Physiol., 59: 270 - 276.
- Gawel, N.J. and Robacker, C.D. (1990). Somatic embryogenesis in two Gossypium hirsutum genotypes on semi-solid versus liquid proliferation media. Plant Cell Tiss. Org. Cult., 23: 201-204.
- Harris, R.E. and Mason, E.B.B. (1983). Two machines for in vitro propagation of plants in liquid media. Can. J. Plant Sci,. 63: 311-316.
- Nene, Y.L., Sheila, V.K. and Moss, J.P. (1996). Tapioca a potential substitute for agar in tissue culture media. Curr. Sci., 70 : 493-494.
- Robinson, J.C. (1996). Banana and Plantains. 104-127 p. CAB International University Press, Cambridge, U.K.
- Skidmore, D.I., Simon, A.J. and Bedis (1988). In vitro culture of shoots of Pinus caribaea on a liquid medium. Plant Cell Tiss. Org. Cult., 14 : 129-136.
- Ziv, M. (2000). Bioreactor technology for plant micropropagation. Hort. Rev., 24: 1-30.
![](https://i-scholar.in/public/site/images/abstractview.png)
Abstract Views: 268
![](https://i-scholar.in/public/site/images/pdfview.png)
PDF Views: 0