International Journal of Plant Sciences

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Estimation of Genetic Diversity in Nutmeg (Myristica fragrans Houtt.) Selections Using RAPD Markers


Affiliations
1 Regional Agricultural Research Station (KAU), Kottayam (Kerala), India
     

   Subscribe/Renew Journal


Nutmeg (Myristica fragrans Houtt.) is an evergreen tree and popular spice. In the present study, Random Amplified Polymorphic DNA (RAPD) analysis was performed to assess the genetic diversity among the 19 superior accessions of nutmeg collected from different geographic locations and maintained in the germplasm collections of Regional Agricultural Research Station, Kumarakom, Kerala. This included one released variety IISR Viswasree. Out of 28 RAPD primers tested, 20 were amplified. Out of the 109 loci amplified, 82 were polymorphic with an average polymorphism rate of 72.74 per cent. The number of bands for each primer ranged from 2 to 8. The markers which produced maximum number of polymorphic bands were BB-18 and PO-5. PIC value of the markers ranging from 0.09 (OPA 11) to 0.48 (W-15) with an average of 0.31. The marker index (MI) varied between 0.09 and 2.08 with an average of 1.25. Jaccard’s similarity co-efficient of the genotypes ranged between 0.34-0.93. Dendrogram constructed based on UPGMA analysis grouped the 19 selected genotypes into two major clusters. The knowledge on genetic diversity of nutmeg can be used for further breeding programmes for getting higher nut and mace yield.

Keywords

Nutmeg, Genetic Diversity, RAPD, Dendrogram.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Anderson, J.A., Churchill, G.A., Autrique, J.E., Tanksley, S.D. and Sorrells, M.E. (1993). Optimizing parental selection for genetic linkage maps.Genome, 36 : 181-186.

  • Asgarpanah, J. and Kazemivash, N. (2012). Phytochemistry and pharmacologic properties of Myristica fragrans Hoyutt.: A review. African J. Biotechnol., 11 (65) : 12787-12793.

  • Chaudhary, Raju, Ghimire, Surya Kant, Joshi, Bal Krishna, Ojha, Bishnu Raj, Niroula, Raj Kumar and Yadav, Pradip Kumar (2016). Genetic diversity in large cardamom (Amomum subulatum Roxb.) dissected using RAPD markers. Internat. J. Adv. Res., 4(6) : 1443-1451.

  • Cruz, V.M. V., Luhman, R., Marek, L.F., Rife, C.L., Shoemaker, R.C., Brummer, E.C. and Gardner, C.A.C. (2007). Characterization of flowering time and SSR marker analysis of spring and winter type Brassica napus L. germplasm. Euphytica, 153 : 43-57.

  • Gajera, B.B., Kumar, N., Singh, A.S., Punvar, B.S., Ravikiran, R., Subhash, N. and Jadeja, G. (2010). Assessment of genetic diversity in castor (Ricinus communis L.) using RAPD and ISSR markers. Industrial Crops Products, 32 : 491-498.

  • Ganeshaiah, K.N., Ravi Shankar, K.V., Anand, Lalitha, Shibu, M. and Shanker, Uma (2000). Identification of sex specific DNA markers in the dioecious tree, nutmeg. Plant Genet. Resour. Newslett., 121:59-61.

  • Jaccard, P. (1908). Nouvellesrecherchessur la distribution florale. Bull. Soc. Vaud. Sci. Nat., 44 : 223-270.

  • Kawar, P.G., Devarumath, R.M. and Nerkar, Y. (2009). Use of RAPD markers for assessment of genetic diversity in sugarcane cultivars. Indian J. Biotechnol., 8:67-71.

  • Krishnan, Anu G., Sabu, Thushara Susan., Jeevan, Madhumol. and Gopinath,Gisheena (2016). Genetic relationship assessment of superior accessions of Garcinia gummi-gutta L. collected from central Travancore regions using RAPD markers. Adv. Res. J. Crop Improv., 7(2):224-230.

  • Kumar, P., Gupta, V.K., Misra, A.K., Modi, P.R. and Pandey, B.K. (2009). Potentials of molecular markers in plant biotechnology. Plant Omics J., 2 : 141-162.

  • Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S. and Rafalski, A. (1996). A comparison of RFLP, RAPD, AFLP and SSR markers for germplasm analysis. Mol. Breed., 2 : 225-238.

  • Radhakrishnan, V.V. and Mohanan, K.V. (2005). Molecular characterisation of some elite genotypes of cardamom (Eleteria cardamomum Maton). Indian J. Genet. Plant Breed., 65 (3) : 227-228.

  • Rohlf, F.J. (1998). NTSys-pc: numerical taxonomy and multivariate analysis system, version 2.02a. Exter Publications, Setauket, NEW YORK, U.S.A.

  • Roldan-Ruiz, I., Calsyn, E., Gilliand, T.J., Coll, R., van Eijk, M.J.T. and De Loose, M. (2000). Estimating genetic conformity between related ryegrass (Lolium) varieties 2. AFLP characterization. Mol. Breed., 6 : 593-602.

  • Salem, H.H., Ali, B.H., Huang, T.H., Qindn,Nang, X.M. and Xie, Q.D.(2007). Use of random amplified polymorphic DNA analysis for economically important food crops. J. Integra. Plant Biol., 49:1670-1680.

  • Sandigawad, A.M. and Patil, C.G. (2011). Genetic diversity in Cinnamomum zeylanicum Blume. (Lauraceae) using random amplified polymorphic DNA (RAPD) markers. African J. Biotechnol., 10 (19) : 3682-3688.

  • Sheeja, T.E., Rajesh, Y., Krishnamoorthy, B. and Parthasarathy, V.A. (2006). DNA polymorphism in clonal and seedling progenies of an elite nutmeg (Myristica fragrans Houtt.) by RAPD. J. Plantn. Crops, 34 : 558-561.

  • Sheeja, T.E., Rajesh, Y., Krishnamoorthy, B. and Parthasarathy, V.A. (2008). Optimization of DNA isolation and PCR parameters in Myristica sp. and related genera for RAPD and ISSR analysis. J. Spices Arom. Crops, 17: 91-97.

  • Sheeja, T.E., Sabeesh, C., Shabna, O.V., Shalini, R.S. and Krishnamoorthy, B. (2013). Genetic diversity analysis of Myristica and related genera using RAPD and ISSR markers. J. Spices Aromatic Crops, 22 (1) : 38-46

  • Sivaprakash, K.R., Prasanth, S.R., Mohanty, B.P. and Parida, A. (2004). Genetic diversity of black gram landraces as evaluated by AFLP markers. Curr. Sci., 86 : 1411-1415.

  • Sneath, P.H.A. and Sokal, R.R. (1973). Numerical taxonomy the principles and practice of numerical classification. Freeman W.H, San Francisco, California,U.S.A. 573 pp.

  • Somani, R.S. and Singhai, A.K. (2008). Hypoglycaemic and antidiabetic activities of seeds of Myristica fragrans in normoglycaemic and Alloxan-induced diabetic rats. Asian J. Exp. Sci., 22 (1) : 95-102.

  • Tharachand, C., Immanuel, S.C. and Abraham, Z. (2015). Molecular insights into the genetic diversity of Garcinia cambogia germplasm accessions. Braz. Arch. Boil. Technol., 58(2):765-772.

  • Uyoh, E.A., Umego, C., and Aikpokpodion, P.O. (2014). Genetic diversity in African nutmeg (Monodora myristica) accessions from South Eastern Nigeria. African J. Biotechnol., 13(42):4105-4111.


Abstract Views: 280

PDF Views: 0




  • Estimation of Genetic Diversity in Nutmeg (Myristica fragrans Houtt.) Selections Using RAPD Markers

Abstract Views: 280  |  PDF Views: 0

Authors

Anu G. Krishnan
Regional Agricultural Research Station (KAU), Kottayam (Kerala), India
Reshma John
Regional Agricultural Research Station (KAU), Kottayam (Kerala), India
Anu Cyriac
Regional Agricultural Research Station (KAU), Kottayam (Kerala), India
G. V. Sible
Regional Agricultural Research Station (KAU), Kottayam (Kerala), India

Abstract


Nutmeg (Myristica fragrans Houtt.) is an evergreen tree and popular spice. In the present study, Random Amplified Polymorphic DNA (RAPD) analysis was performed to assess the genetic diversity among the 19 superior accessions of nutmeg collected from different geographic locations and maintained in the germplasm collections of Regional Agricultural Research Station, Kumarakom, Kerala. This included one released variety IISR Viswasree. Out of 28 RAPD primers tested, 20 were amplified. Out of the 109 loci amplified, 82 were polymorphic with an average polymorphism rate of 72.74 per cent. The number of bands for each primer ranged from 2 to 8. The markers which produced maximum number of polymorphic bands were BB-18 and PO-5. PIC value of the markers ranging from 0.09 (OPA 11) to 0.48 (W-15) with an average of 0.31. The marker index (MI) varied between 0.09 and 2.08 with an average of 1.25. Jaccard’s similarity co-efficient of the genotypes ranged between 0.34-0.93. Dendrogram constructed based on UPGMA analysis grouped the 19 selected genotypes into two major clusters. The knowledge on genetic diversity of nutmeg can be used for further breeding programmes for getting higher nut and mace yield.

Keywords


Nutmeg, Genetic Diversity, RAPD, Dendrogram.

References