International Journal of Plant Sciences

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Evaluation Study of Micropropagation Stages of Patchouli Plant


Affiliations
1 Department of Biotechnology, Parul Institute of Applied Sciences, Parul University, Limda, Waghodia, Vadodara (Gujarat), India
2 Department of Biotechnology, Parul Institute of pplied Sciences, Parul University, Limda, Waghodia, Vadodara (Gujarat), India
3 Department of Biochemistry, Parul Institute of Medical and Research, Parul University, Limda, Waghodia, Vadodara (Gujarat), India
4 College of Agricultural Biotechnology, Loni, Pravaranagar (M.S.), India
5 Parul Institute of Applied Sciences, Parul University, Limda, Waghodia, Vadodara (Gujarat), India
     

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An effective means for rapid multiplication of plant species of clonal origin is micro propagation. Various in vitro studies have been reported on different species of patchouli, one such method of propagation that can be usefully employed to produce relatively uniform plantlets in a short time is via in vitro culture but there is limited effort to study direct organogenesis, which supports cultivation by providing true type plants in large numbers.Therefore this study determines the effect of different concentrations of growth hormones on patchouli, micropropagation and rapid multiplication stages of patchouli plant within a short time with good results of micropropagation stages for regeneration of patchouli were successfully initiated.

Keywords

Patchouli, Alpha Naphthalene Acetic Acid (NAA), Indol Acetic Acid (IAA), Benzyl Amino Purine (BAP), Kinetin (kin).
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  • Bauer, K., Garve, D. and Surburg, H. (1997). Common fragrance and flavor materials:Prepration, properties and used, Wiley-VCH, Weinheim, 3 : 205.

  • Famiani, F., Ferradini, N., Staffolani, P. and Standardi, A. (1994). Effect of leaf excision time and age, BA concentration and dark treatments on in vitro shoot regeneration of M26 apple ischolar_mainstock. J. Hort. Sci., 69, 679-685.

  • Hasegawa, Y., Tajama, K., Toi, N. and Sugimura, Y. (1995). Plant regeneration from patchouli protopasts encapsulated in alginate beads. Plant Cell Tiss. Org. Cult., 41 (1) : 65-70.

  • Hirata, T., Murakami, S., Ogihara, K. and Suga, T. (1990). Volatile monoterpenoid constituents of the plantlets of Mentha spicata produced by shoot tip culture. Phytochemistry, 29 : 493-495.

  • Jones, L.H. and Krishnadethan, P.P.S. (1973). Factors influencing production of patchouli sesquiterpenes in cultured cells and regenerated plantlets. Phytochemistry, 12 : 1513-1514.

  • Kageyama, Y., Honda, Y. and Sugimura, Y. (1995). Plant regeneration from patchouli protoplasts encapsulated in alginate beads. Plant Cell Tiss.Organ Cult., 41 : 65-70.

  • Kukreja, A.K., Mathur, A.K. and Zaim, M. (1990). Mass production of virus-free patchouli plants [Pogostemon cablin (Blanco) Benth.] by in vitro culture. Trop. Agric., 67 : 101-104.

  • Liu, X. and Pijut, P.M. (2008). Plant regeneration fromin vitro leaves of mature blackcherry (Prunus serotina). Plant Cell Tiss.Organ Cult., 94 : 113-123.

  • Maheshwari, M.L., Vasantha, K.T., Neelam, S. and Chadel, K.P.S. (1993). Patchouli-An Indian perspective. Indian Perfumer, 37 : 9-11.

  • Meena, M. (1996). Regeneration of patchouli (Pogostemon cablin Benth.) plants from leaf and node callus and evolution after growth in the field. Plant Cell Rep., 15 : 991-994.

  • Obul, R.B., Giridhar, P. and Ravishankar, G.A. (2001). In vitro ischolar_maining of Decalepis hamiltonii. Wight and Arn, an endangered shrub, by auxins and ischolar_main promoting agents. Curr. Sci., 81(11):26-29.

  • Park, E., Yoon, H.K. and Kim, D.H. (2002). Antithrombotic activity of sunghyangjunggisan. Natural Products Sci., 8(2):71-75.

  • Paula, C., Alexandra, C. and Conceicao, S. (2008). A protocol for Ulmus minor Mill. Micropropafation and acclimatization. Plant Cell Tiss.Org.Cult., 92 (1):113-119.

  • Perez-Tornero, O., Egea, J., Vanoostende, A. and Burgos, L. (2000). Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot. Plant Sci., 158 : 61-70.

  • Reddy, P.S., Rodrigues, R. and Rajasekharan, R. (2001). Shoot organogenesis and mass propagation of Coleus forskohlii from leaf derived callus. Plant Cell Tiss. Org. Cult., 66 : 183-188.

  • Sankhla, D., Davis, D.T., Sankhla, N. and Upadhyaya, A. (1993). Clonal multiplication of Germanred carnation. Proc. Plant Growth Reg. Soc. Am., 20 : 167-174.

  • Sha-Valli-Khan, P.S., Prakash, E. and Rao, K.R. (2002). Callus induction and plantlet regeneration in Bixa oreliana L., an annatto-yielding tree. In vitro Cell. Dev. Biol.Plant, 38 (2) : 186-190.

  • Sharma, R.N., Gupta, A.S., Patwardhan, S.A., Hebbalkar, D.S. and Tare, V. (1992). Bioactivity of Lamiaceae plants against insect’s. Indian J.Exp.Biol., 30 : 244-246.

  • Sudria, C., Pinol, M.T., Palazon, J., Cusido, R.M., Vila, R., Morales, C., Bonfill, M. and Canigueral, S. (1999). Influence of plant growth regulators on the growth and essential oil content of cultured Lavandula dentata plantlets. Plant Cell Tiss. Organ Cult., 58 : 177-184.

  • Sugimora,Y.M., Padayhag, S., Ceniza, K.N., Eguchi, S., Natsuaki, T. and Okuda, S. (1995). Essential oil production increased by using virus free patchouli plants derived from meristem-tip culture. Plant Pathol., 44 : 510-515.

  • Sumi, H. (2003). Fibrinolysis-accelerating activity of the essential oils and Shocho aroma. Aroma Res., 4(3) : 264-267.

  • Sun, Y., Zhao, Y., Wang, X., Qiao, G., Chen, G., Yang, Y., Zhou, J., Jin, L. and Zhuo, R. (2009). Adventitious bud regeneration from leaf explants of Platanus occidentalis L. and genetic stability assessment. Acta Physiol. Plant., 31 : 33-41.

  • Thomas, T.D. (2007). High-frequency, direct bulblet induction from rhizome explants of Curculigo orchioides Gaertn. An endangered medicinal herb. In Vitro Cell. Dev. Biol., 43 : 442-448.

  • Yang, D., Michel, Madin, Chaumont, Andriamboavonjypoitry and Mellet, C. (1996). Antifungal and antibacterial properties in vitro of three patchouli oils from different origins. Acta Botanica Gallica, 14 (2):29-35.

  • Yukio, S., Naoto, K., Yoshiko, Kaori, S., Sakihito, K., Toshiharu, F. and Masato, I. (2005). Tansgenic patchouli plants produced byAgrobacterium-mediated transformation. Plant Cell, Tissue & Organ Culture, 82 : 251-257.


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  • Evaluation Study of Micropropagation Stages of Patchouli Plant

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Authors

Sailaja Inampudi
Department of Biotechnology, Parul Institute of Applied Sciences, Parul University, Limda, Waghodia, Vadodara (Gujarat), India
Laxman Bhosale
Department of Biotechnology, Parul Institute of Applied Sciences, Parul University, Limda, Waghodia, Vadodara (Gujarat), India
Anuradha Rohinikar
Department of Biotechnology, Parul Institute of pplied Sciences, Parul University, Limda, Waghodia, Vadodara (Gujarat), India
Ivvala Anand Shaker
Department of Biochemistry, Parul Institute of Medical and Research, Parul University, Limda, Waghodia, Vadodara (Gujarat), India
C. Gonjari Komal
College of Agricultural Biotechnology, Loni, Pravaranagar (M.S.), India
Ajit Gangavane
Parul Institute of Applied Sciences, Parul University, Limda, Waghodia, Vadodara (Gujarat), India

Abstract


An effective means for rapid multiplication of plant species of clonal origin is micro propagation. Various in vitro studies have been reported on different species of patchouli, one such method of propagation that can be usefully employed to produce relatively uniform plantlets in a short time is via in vitro culture but there is limited effort to study direct organogenesis, which supports cultivation by providing true type plants in large numbers.Therefore this study determines the effect of different concentrations of growth hormones on patchouli, micropropagation and rapid multiplication stages of patchouli plant within a short time with good results of micropropagation stages for regeneration of patchouli were successfully initiated.

Keywords


Patchouli, Alpha Naphthalene Acetic Acid (NAA), Indol Acetic Acid (IAA), Benzyl Amino Purine (BAP), Kinetin (kin).

References