![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_open_medium.gif)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_restricted_medium.gif)
Frequency and Spectrum of Chlorophyll Mutants Induced by Gamma Rays and EMS in Two Chickpea Varieties (Variety-Vijay and PKV-2)
Subscribe/Renew Journal
Chickpea is one of the most important leguminous food grain grown worldwide. Mutagenesis could be used as a classical way to increase genetic variability in chickpea considering its narrowing genetic base. Present study was an attempt to analyze the frequency and spectrum of chlorophyll mutations induced by gamma rays (300, 400 and 500 Grey) and ethyl methanesulphonate (0.2%, 0.3% and 0.4%) in M2 generation of varieties of chickpea (Var-Vijay and PKV-2). Broad spectrum chlorophyll mutants were isolated from M2 generation. The relative frequency of these mutants in both varieties was observed in order of Tigrina > Viridis > Chlorina > Xantha and Albina. The total chlorophyll mutation frequency increased with increase of dose upto certain limit and then started declining. However, the effectiveness of EMS was found to be more prominent than gamma rays especially in inducing chlorophyll mutations in Chickpea.
Keywords
Chickpea, Chlorophyll Mutants, Ethyl Methanesulphonate, Gamma Rays, Frequency.
Subscription
Login to verify subscription
User
Font Size
Information
- Ahsan-ul-Haq, M., Singh, K.B., Abidin, Z. and Ahemad, M.S. (1994). Mutation study in chickpea (Cicer arientinum L.) III : Selection of mutants in M2 generation. Pak. J. Agri. Sci. 31(1): 13-22.
- Barshile, J. and Boddu, A. (2012). Genetic improvement of chickpea (Cicer arientinum L.) cultivar Vijay (Phule G 81-1-1-1) through induced mutagenesis. Bioremed. Biodivers. Bioavail., 6(1): 103-106.
- Choudhary, P., Khanna, S.M., Jain, P.K., Bharadwaj, C., Kumar, K., Lakhera, P.C. and Srinivasan, K. (2013). Molecular characterization of primary gene pool of chickpea based on ISSR markers. Biochemical Genetics, 51: 306-322.
- FAOSTAT (2013). Food and Agriculture Organization of the United Nations, FAOSTATdatabase available at http://faostat3.fao.org/faostat-gateway/go/to/home/E. Accessed on 7 Jan 2015.
- Gaul, H. (1964). Mutations in plant breeding. Radiat. Bot., 4(3): 155-232.
- Gaur, P.M. and Gour, V.K. (2003). Broad-few-leaflets and outwardly curved wings: two new mutants of chickpea. Plant Breeding, 122: 192-197.
- Gustafsson, A. (1940). A mutation system of chlorophyll apparatus. Lund University. Arsskr NF. Adv., 36: 1-40.
- Haq, M.A., M. Sadiq, M., Hassan, T.M. Shah and Hina, S. (2002). CM 2000-A new Kabuli chickpea variety. Pak. J. Seed Tech., 1(1): 45-49.
- Joshi-Saha, A. and Reddy, K.S. (2014). Diversity study in chickpea (Cicer arietinum L.) using REMAP, SSR and ISSR markers. J. Food Legumes, 27: 281-288.
- Joshua, D.C. (2000). Guidelines for successful mutation breeding. In: Proceedings of the DAE-BRNS symposium on the use of nuclear and molecular techniques in crop improvement. Mumbai, India. pp 21-26.
- Jukanti, A.K., Gaur, P.M. Gowda, C.L.L. and Chibbar, R.N. (2012). Nutritional quality and health benefits of chickpea (Cicer arietinum L.): a review. British J. Nutr., 108: S11-S26.
- Kaul, M.L.H. and Bhan, A.K. (1977). Mutagenic effectiveness and efficiency of EMS, DES and gamma rays in rice. Theor. & Appl. Genetics, 50: 241-246.
- Khan, S. and Siddiqui, B.A. (1992). Mutagenic effectiveness and efficiency of chemical mutagens in Vigna radiata (L.) Wilczek. Thai J Agric. Sci., 25 : 291-297.
- Khan, S., Wani, M.R. and Parveen, K. (2004). Induced genetic variability for quantitative traits in Vigna radiata (L.) Wilczek. Pakistan J Bot., 36: 845-50.
- Khan, S., Wani, M.R., Bhat, M. and Koursar, P. (2005). Induced chlorophyll mutations in chickpea (Cicer arietinum L.). Internat. J Agric. Biol., 7(5) : 764-767.
- Kharkwal, M.C. (1998). Induced mutations in chickpea (Cicer arietinum L.) II. Frequency and spectrum of chlorophyll mutations. Indian J. Genetics, 58 : 465-474.
- Kharkawal, M.C. (2003). Induced mutations in chickpea (Cicer arietinum L.). VI. Significance of induced altered correlations. Indian J. Genet. & Pl. Breed., 63 (3) : 219-224.
- Koli, N.R. and K. Ramkrishna. 2002. Frequency and spectrum of induced mutations and mutagenic effectiveness and efficiency in fenugreek (Trigonella foenumgraecumL.). Indian J. Genet., 62(4): 365-366.
- Konzak, C.F., Nilan, R.A., Wagner, J. and Foster, R.J. (1965). Efficient chemical mutagenesis. Radiation Bot. (Suppl.), 5 : 49-70.
- Loyavar, R. Shunmughvalli, N, Mutthuswamy and Rajesh, S. (2017). Induced mutagenic frequency and spectrum of chlorophyll mutants in Black gram, Vigna mungo (L.) Hepper. Internat. J. Farm Sci., 7 (1) : 19-22.
- Maluszynski, K.N., Zanten, L.V. and Ahlowalia, B.S. (2000). Officially released mutant varieties- The FAO/IAEA Database. Mut. Breed. Rev., 12:1-12.
- Sharma, S., Upadhayaya, H.D., Varshney, R.K. and Gowda, C.L.L. (2013). Prebreeding for diversification of primary gene pool and genetic enhancement of grain legumes. Frontiers Plant Sci., 4 : 309.
- Swaminathan, M.S., Chopra, V.L. and Bhaskaran, S. (1962). Chromosome aberrations and the frequency and spectrum of mutations induced by ethymethane sulphonate in barley and wheat. Indian J. Genet., 22 : 192-207.
- Toker, C. and Cagigran, M.I. (2004). Spectrum and frequency of induced mutations in chickpea. ICPN, 11 : 20-21.
- Wani, A.A. (2009). Mutagenic effectiveness and efficiency of gamma rays, ethyl methane sulphonate and their combination treatments in chickpea (Cicer arietinum L.). Asian J. Plant Sci., 8 : 318-321.
![](https://i-scholar.in/public/site/images/abstractview.png)
Abstract Views: 323
![](https://i-scholar.in/public/site/images/pdfview.png)
PDF Views: 0