International Journal of Plant Sciences

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Screening of Acquired Thermotolerant Ragi [Eluesina coracana (L.) Gaertn] Genotypes Using T.I.R. Technique


Affiliations
1 Department of Botany, Andhra University, Visakhapatnam (A.P.), India
2 Department of Botany, Govt. Junior College, Gurla, Vizianagaram (A.P.), India
3 Department of Botany, Government Degree College, Saluru, Vizianagaram (A.P.), India
     

   Subscribe/Renew Journal


For present population ragi is the major food as it is considered as the power house of health benefits. The production of ragi is coming down slowly due to the climatic factors like temperature and drought. Breeding of selected genotypes with increased thermotolerance is therefore, one of the most vital objective in crop improvement programme. Temperature induction response (TIR) technique has been developed to identify thermotolerant lines. 24 ragi genotypes has been tested using temperature induction response (TIR) technique. Ragi seedlings were exposed to gradual increase in temperature range of 32-48°C for 5hrs and later subjected to the lethal temperature of 54°C for 2 hrs. These treated seedlings were allowed to recover at 30°C and 60% relative humidity for 2 days. After recovery per cent survival, per cent reduction of ischolar_main growth and per cent reduction of shoot growth was calculated. Among 24 ragi genotypes VR900, Indaf 8 and Udurumalliga were found resistant with low per cent reduction of ischolar_main and shoot growth and the genotypes VR 1138, CO-7 and OUAT-2 were found susceptible with high per cent reduction of ischolar_main and shoot growth. By using this TIR technique it is easy to identify thermotolerant lines from a large range of population at the seedling level itself.

Keywords

Acquired Thermotolerance, Temperature Induction Response, Lethal Temperature.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Burke, J.J.(2001).Identification of genetic diversity and mutations in higher plant acquired thermotolerance. Physiologia Plantarum, 112(2) : 167-170.

  • Chandola, Ankita, Pandurangam, Vijai and Srivastava, Jai Prakash (2016). Genotypic variations in tomato (Lycopersicon esculentum Mill.) for acquired thermotolerance to temperature induction response. Internat. J. Agric., Environ. &Biotechnol., 9(4):649-657.

  • Gangappa, E., Ravi, K. and Kumar, N. Veera (2006). Evaluation of groundnut (Arachis hypogeae L.) genotypes for temperature tolerance based on temperature induction response (TIR) technique. Indian J. Genetics, 66(2):127-130.

  • Gomathi, R., Siyamala, S., Vasantha, S. and Suganya, A. (2014). Optimization of temperature conditions for screening thermotolerance in sugarcane through temperature induction response (TIR) technique. Internat. J. Sci., 3 : 5-18.

  • Harihar, Sapna, Srividya, S., Vijayalakshmi, C. and Boominathan, P. (2014) .Temperature induction response – A physiological approach to identify thermotolerant genoypes in rice. Internat. J. Agric. Sci., 10 : 230-232.

  • Jayaprakash, T.L., Ramamohan, G., Krishna Prasad, B.T., Ganesh Kumar, Prasad, T.G., Mathew, M.K. and Udaya Kumar M. (1998). Genotypic variability in differential expression of Lea2 and Lea3 genes and proteins in response to salinity stress in finger millet (Eleusine coracona Gaertn) and rice (Oryza sativa L.) seedlings. Ann. Bot., 82 : 513–522.

  • Kheir, Ehab Abou, Sheshshayee, M.S., Prasad, T.G. and Udayakumar, M. (2012). Temperature induction response as a screening technique for selecting high temperature-tolerant cotton lines. J. Cotton Sci., 16 : 190-199.

  • Krishnan, M., Nguyen, H.T. and Burke, J.J. (1989). Heat shock protein synthesis and thermotolerance in wheat. Plant Physiol., 90 : 140–145.

  • Kumar, G.T., Krishnaprasad, B.T., Savitha, M., Gopalakrishna, R., Mukhopadyay, K., Ramamohan, G. and Udayakumar, M. (1999). Enhanced expression of heat-shock protiens in thermos-tolerant lines of sunflower and their progenies selected on the basis of temperature induction response. Theor. Appl. Genet., 99 : 359-367.

  • Renuka Devi, K., Siva Shankar, A. and Sudhakar, P. (2013). Sceening of rice genotypes for thermotolerance by TIR technique. Internat. J. Appl. Biol. &Pharmaceu. Technol., 4(4):205-210.

  • Selvaraj, M.G., Burow, G., Burke, J.J., Belamkar, V., Puppala, N. and Burrow, M.D. (2011). Heat stress screening of peanut (Arachis hypogaea L.) seedlings for acquired thermotolerance. Plant Growth Regulation, 65: 83-91.

  • Senthil-Kumar, M., Srikanthbabu, V., Mohan Raju, B., Ganeshkumar, Shivaprakash, N. and Udayakumar, M. (2003). Screening of inbred lines to develop thermotolerant sunflower hybrid using the temperature induction response (TIR) technique: a novel approach by exploiting residual variability. J. Experimental Bot., 54(392) : 2569-2578.

  • Senthil-Kumar, Muttappa, Kumar, Ganesh, Venkatachalayya Srikanthbabu and Udayakumar, Makarla (2007). Assessment of variability in acquired the rmotolerance: Potential option to study genotypic response and the relevance of stress genes. J. Plant Physiol., 164:111-125.

  • Srikanth Babu, V., Ganesh Kumar, Krishnaprasad, B.T., Gopalaswamy, R., Savitha, M. and Udayakumar, M. (2002). Identification of pea genotypes with enhanced thermotolerance using temperature induction response technique (TIR). J. Plant Physiol., 159 : 535-545.

  • ___Sub – sectoral analysis of Ragi.

  • Sudhakar, P., Latha, P., Rameshbabu, Sujatha, K and Raja Reddy. (2012). Screening of paddy genotypes for thermotolerance to using TIR technique in pursuit of global warming. Indian journal of Plant Physiology. (In Press).

  • Uma, S., Prasad, T. G. and Kumar, M.U.(1995).Genetic variability in recovery growth and synthesis of stress protiens in response to polyethylene glycol and salt stress in finger millet. Ann. Bot., 76(1) : 43-40.

  • Uwimana Marie Ange, Srividhya, S., Vijayalakshmi, C. and Boominathan, P. (2016). Temperature induction response reveals intrinsic thermotolerant genotypes in soyabean. Legume Res., 39 (6):926-930.

  • Venkatesh Babu, D., Sudhakar, P. and Reddy, Y. Sharath Kumar (2013). Screening of thermotolerant Ragi genotypes at seedling stage using TIR technique. Bioscan, 8(4): 1493-1495.

  • Vierling, E. (1991). The roles of heat shock protiens in plants. Annu. Rev. Plant Physiol. & Plant Molecular Biol., 42 : 579-620.

  • Vijayalakshmi, D., Srividya, S., Vivitha, P. and Raveendran, M. (2015). Temperature induction response (TIR) as a rapid sscreening protocol to dissect the genetic variability in acquired thermotolerance in rice and to identify novel donors for high temperature stress tolerance. Indian J. Plant Physiol., 20 (4):368-374.


Abstract Views: 271

PDF Views: 0




  • Screening of Acquired Thermotolerant Ragi [Eluesina coracana (L.) Gaertn] Genotypes Using T.I.R. Technique

Abstract Views: 271  |  PDF Views: 0

Authors

B. Sujatha
Department of Botany, Andhra University, Visakhapatnam (A.P.), India
P. Sirisha
Department of Botany, Govt. Junior College, Gurla, Vizianagaram (A.P.), India
Y. V. Bharathi
Department of Botany, Government Degree College, Saluru, Vizianagaram (A.P.), India

Abstract


For present population ragi is the major food as it is considered as the power house of health benefits. The production of ragi is coming down slowly due to the climatic factors like temperature and drought. Breeding of selected genotypes with increased thermotolerance is therefore, one of the most vital objective in crop improvement programme. Temperature induction response (TIR) technique has been developed to identify thermotolerant lines. 24 ragi genotypes has been tested using temperature induction response (TIR) technique. Ragi seedlings were exposed to gradual increase in temperature range of 32-48°C for 5hrs and later subjected to the lethal temperature of 54°C for 2 hrs. These treated seedlings were allowed to recover at 30°C and 60% relative humidity for 2 days. After recovery per cent survival, per cent reduction of ischolar_main growth and per cent reduction of shoot growth was calculated. Among 24 ragi genotypes VR900, Indaf 8 and Udurumalliga were found resistant with low per cent reduction of ischolar_main and shoot growth and the genotypes VR 1138, CO-7 and OUAT-2 were found susceptible with high per cent reduction of ischolar_main and shoot growth. By using this TIR technique it is easy to identify thermotolerant lines from a large range of population at the seedling level itself.

Keywords


Acquired Thermotolerance, Temperature Induction Response, Lethal Temperature.

References