International Journal of Plant Sciences

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Study on Induced Mutations in M1 Generation in Sorghum [Sorghum bicolor (l.) Moench ]


Affiliations
1 Department of Agricultural Botany, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India
2 Upland Paddy Research Scheme, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India
     

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An investigation was carried out to create the variability generated through induced mutation in two sorghum populations viz., 296 B (Kharif) and Parbhani Moti (Rabi). Two mutagens viz., gamma irradiation (10 kR, 20kR, 30kR and 40kR) and EMS (0.1%EMS, 0.2%EMS, 0.3%EMS and 0.3%EMS) and their combination were used M1 generation. Mutagenic sensitivity in M1 generation on the basis of reduced germination and plant survival revealed a dose dependent reaction and differential response of the populations. LD50 was found to be 20-30 kR in case of gamma irradiation and 0.3-0.4 per cent in EMS irrespective of the genotype. The irradiated population produced more number of superior segregants in respects of seed yield and its contributing traits compared to other populations. Three dwarf mutant, one brown midrib and tree drought tolerance confirmed from Parbhani Moti.

Keywords

Mutation, Segregants, EMS, LD50 ,Genetic Variability.
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  • Study on Induced Mutations in M1 Generation in Sorghum [Sorghum bicolor (l.) Moench ]

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Authors

S. M. Surashe
Department of Agricultural Botany, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India
H. V. Kalpande
Department of Agricultural Botany, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India
S. B. Borgaonkar
Upland Paddy Research Scheme, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India

Abstract


An investigation was carried out to create the variability generated through induced mutation in two sorghum populations viz., 296 B (Kharif) and Parbhani Moti (Rabi). Two mutagens viz., gamma irradiation (10 kR, 20kR, 30kR and 40kR) and EMS (0.1%EMS, 0.2%EMS, 0.3%EMS and 0.3%EMS) and their combination were used M1 generation. Mutagenic sensitivity in M1 generation on the basis of reduced germination and plant survival revealed a dose dependent reaction and differential response of the populations. LD50 was found to be 20-30 kR in case of gamma irradiation and 0.3-0.4 per cent in EMS irrespective of the genotype. The irradiated population produced more number of superior segregants in respects of seed yield and its contributing traits compared to other populations. Three dwarf mutant, one brown midrib and tree drought tolerance confirmed from Parbhani Moti.

Keywords


Mutation, Segregants, EMS, LD50 ,Genetic Variability.

References