International Journal of Plant Sciences

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Study on Mutagenic Effectiveness and Efficiency of Mutagens in Inducing Chlorophyll Mutations in M2 Generation in Sorghum [Sorghum bicolor (l.) Moench ]


Affiliations
1 Department of Agricultural Botany, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India
2 Upland Paddy Research Scheme, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India
     

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An investigation was carried out to create the variability generated through induced mutation in two sorghum populations viz., 296 B (Kharif) and Parbhani Moti (Rabi). Two mutagens viz., gamma irradiation (10 kR, 20kR, 30kR and 40kR) and EMS (0.1%EMS, 0.2%EMS, 0.3%EMS and 0.3%EMS) and their combination were used M2 generation. Mutagenic sensitivity in M2 generation on the basis of reduced germination and plant survival revealed a dose dependent reaction and differential response of the populations. In general, chlorophyll mutation frequency expressed on M2 seedling basis increased linearly with doses of three the mutagens in 296 B and Parbhani Moti. The frequency was more in 296 B followed by Parbhani Moti of three mutagens. The spectrum of chlorophyll mutations comprised albina, xantha, viridis, xanthaviridis, chlorina. The most frequently occurred mutant was viridis type followed by chlorina in all the populations. The population 296 B had expressed largest frequency of chlorophyll mutants followed by Parbhani Moti.

Keywords

Mutation, Segregants, EMS, LD50 ,Genetic variability, Albina, Xantha, Chlorina.
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  • Study on Mutagenic Effectiveness and Efficiency of Mutagens in Inducing Chlorophyll Mutations in M2 Generation in Sorghum [Sorghum bicolor (l.) Moench ]

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Authors

S. M. Surashe
Department of Agricultural Botany, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India
H. V. Kalpande
Department of Agricultural Botany, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India
S. B. Borgaonkar
Upland Paddy Research Scheme, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), India

Abstract


An investigation was carried out to create the variability generated through induced mutation in two sorghum populations viz., 296 B (Kharif) and Parbhani Moti (Rabi). Two mutagens viz., gamma irradiation (10 kR, 20kR, 30kR and 40kR) and EMS (0.1%EMS, 0.2%EMS, 0.3%EMS and 0.3%EMS) and their combination were used M2 generation. Mutagenic sensitivity in M2 generation on the basis of reduced germination and plant survival revealed a dose dependent reaction and differential response of the populations. In general, chlorophyll mutation frequency expressed on M2 seedling basis increased linearly with doses of three the mutagens in 296 B and Parbhani Moti. The frequency was more in 296 B followed by Parbhani Moti of three mutagens. The spectrum of chlorophyll mutations comprised albina, xantha, viridis, xanthaviridis, chlorina. The most frequently occurred mutant was viridis type followed by chlorina in all the populations. The population 296 B had expressed largest frequency of chlorophyll mutants followed by Parbhani Moti.

Keywords


Mutation, Segregants, EMS, LD50 ,Genetic variability, Albina, Xantha, Chlorina.

References