International Journal of Plant Sciences

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Effect of Storage on Biochemical Changes in Groundnut (Arachis hypogaea L.) during Kharif and Summer


Affiliations
1 Department of Agricultural Botany, Anand Agricultural University, Anand, Gujarat, India
2 Department of Agricultural Botany, B.A. College of Agriculture, Anand Agricultural University, Anand, Gujarat, India
     

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The biochemical changes during storage were studied in a set of fifty five spreading and semi spreading types of groundnut genotypes (Arachis hypogea L.). The studies indicated the effect of season and storage periods on these parameters. The genotypes behaved differently in different seasons and under different storage periods. Under storage both oil and protein showed marked reduction in their content. The variations were clearly observed in SDS PAGE analysis of protein and esterase isozymes. In the protein analysis 769 bands were observed in fresh Kharif 2010 seed whereas 696 bands were observed in stored summer 2010 seeds. The PIC (Polymorphism information content) of both summer and Kharif 2010 seed was found to be 0.94. Esterase analysis showed 181 bands in the fresh Kharif 2010 seed while, 101 bands were recorded in stored summer 2010 seed. The lower PIC value observed in Kharif and summer seasons for esterase isozymes as compared to proteins indicated that genotypic characterisation using the latter may be more reliable than former.

Keywords

Banding Patterns, Isozymes, Oil Content, Protein Content, Storage Period
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  • Effect of Storage on Biochemical Changes in Groundnut (Arachis hypogaea L.) during Kharif and Summer

Abstract Views: 297  |  PDF Views: 0

Authors

Santosh Kshirsagar
Department of Agricultural Botany, Anand Agricultural University, Anand, Gujarat, India
N. Sasidharan
Department of Agricultural Botany, B.A. College of Agriculture, Anand Agricultural University, Anand, Gujarat, India
Kalyani Kumari
Department of Agricultural Botany, Anand Agricultural University, Anand, Gujarat, India
Bhushan Aher
Department of Agricultural Botany, Anand Agricultural University, Anand, Gujarat, India

Abstract


The biochemical changes during storage were studied in a set of fifty five spreading and semi spreading types of groundnut genotypes (Arachis hypogea L.). The studies indicated the effect of season and storage periods on these parameters. The genotypes behaved differently in different seasons and under different storage periods. Under storage both oil and protein showed marked reduction in their content. The variations were clearly observed in SDS PAGE analysis of protein and esterase isozymes. In the protein analysis 769 bands were observed in fresh Kharif 2010 seed whereas 696 bands were observed in stored summer 2010 seeds. The PIC (Polymorphism information content) of both summer and Kharif 2010 seed was found to be 0.94. Esterase analysis showed 181 bands in the fresh Kharif 2010 seed while, 101 bands were recorded in stored summer 2010 seed. The lower PIC value observed in Kharif and summer seasons for esterase isozymes as compared to proteins indicated that genotypic characterisation using the latter may be more reliable than former.

Keywords


Banding Patterns, Isozymes, Oil Content, Protein Content, Storage Period