International Journal of Plant Sciences

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Callus formation in Ferula jaeschkeana Vatke


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1 Department of Botany, Government College for Women, Gandhi Nagar, Jammu (J&K), India
     

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Callus was initiated from the petiole explants of Ferula jaeschakeana on a medium containing 2,4-D. The highest percentage of explants forming callus (92.5) and callus index (258) were obtained on medium supplemented with 1 mgl-1 2,4-D. Among explants sizes and type tested for callus initiation, 10 mm long petiole gave maximum callus index. Small and large explants initiated a slight amount of callus. Addition of Kn in combination with 2,4-D enhanced the callus formation from the explants.

Keywords

Callus, Ferula jaeschkeana, Tissue Culture.
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  • Arumugam, N. and Bhojwani, S.S. (1990). Somatic embryogenesis in tissue culture of Podophyllum hexandrum. Can. J. Bot., 68:487-491.

  • Bhojwani, S.S. and Razdan, M.K. (2005). Plant tissue culture; theory and practice,-A revised Ed. Published by Elsevier India Pvt. Ltd., Lajpat Nagar IV NEW DELHI, INDIA.

  • Cochran, W.G. and Cox, G.M. (1957). Experimental designs. John Wiley and Sons, NEW YORK, U.S.A.

  • Constabel, F. (1984). Callus Culture: Induction and maintenance. In: Vasil, IK.(Ed.) Cell culture and somatic cell genetics of plants, vol.1, Academic Press, INC, London. pp. 27-35.

  • Dubay, N.K., Kumar, R. and Tripathi, P. (2004). Global promotion of herbal medicines: India's opportunity. Curr. Sci., 86: 37- 41.

  • George, E.F. (1993). Plant propagation by tissue culture. Exegetics Ltd. Edington, Wilts England.

  • Hussain, A., Virmani, O.P., Sharma, A., Kumar, A. and Misra, L.N. (1988). Major essential oil bearing plants in India. Central Institute of Medicinal and Aromatic Plants, Lucknow (U.P.) INDIA.

  • Ibaraki, Y., Matsushima and Kurat, K. (2000). Analysis of morphological changes in carrot somatic embryogenesis by serial observation. Plant Cell Tiss. Org. Cult.,61:4-14.

  • Irvani, N., Solouki, M., Omidi, M. Zare,A.R. and Shahnazi, S. (2010). Callus induction and plant regeneration in Dorema ammoniacum A.- an endangered medicinal plant. Plant Cell Tiss. Org. Cult., 100 : 293-299.

  • Krens, F.A. and Jamar (1989). The role of explants source and culture conditions on callus induction and shoot regeneration in sugarbeet(Beta vulgaris L). J. Plant Physiol., 134: 651-655.

  • Krishnan, P.N., Sudha, C.G. and Seeni,S.C. (1995).Rapid propagation through shoot tip culture of Trichopus zeylanicus Gaertn. - a rare ethnomedicinal plant. Plant Cell Reports, 14: 708- 711.

  • Lewis, W.M. and Elvin-lewis, M.P.F. (2003). Medicinal botany, plants affecting human health. John Wiley & Sons Inc. New Jersey (IInd Ed.) pp. 561.

  • Masoumian, M., Arbakariya, A., Syahida, A. and Maziah, M. (2011). Flavonoid production in Hydrocotyle bonariensis callus tissues. J. Med. Plant Res. 5 : 1564-1574.

  • Murgashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant, 15: 473-497.

  • Nagari, S. and Wakhlu, A.K. (1989). Karyotypic studies of plants regenerated from cultured tissues of fennel (Foeniculum vulgare Miller). In: Manna, G.K.& Sinha, U. (Ed.) Perspect. Cytol. & Genet., 6 : 155-158.

  • Naidu, T.B., Rao, S.N. and Mani, N.S. (2010). Conservation of endangered medicinal plants Centella asiatica through tissue culture. Drug Invention Today, 2: 17-21.

  • Pola. S., Sardadamani, N. and Ramana, T. (2007). Enhanced shoot regeneration in Sorghum bicolor. J. Agric. Technol., 3: 275-286.

  • Valizadeh, M., Safernejad, A., Nematzadeh, G.H. and Kazemitabar, S.K. (2008). Regeneration of plantlets from embryo explants of Bunium persicum (Boiss) B. Fedtsch. J. Sci. & Technol. Agric. & Natural. Resou., 11: 122-129.

  • Wakhlu, A.K. and Sharma, R.K. (1998). Micropropagation of Heracleum candicans Wall: a rare medicinal plant. In vitro Cell. Dev. Biol.-Plant, 35 : 79-81.

  • Wakhlu, A.K. and Sharma, R.K. (2001). Adventitious shoot regeneration from petiole explants ofHeracleum candicans Wall. In vitro Cell. Dev. Biol., 37 : 794-797.

  • Wakhlu, A.K., Nagari, S. and Barna, K.S.(1990). Somatic embryogenesis and plant regeneration from callus cultures of Bunium persicum Boiss. Plant Cell Reports, 9:137-138.

  • Zare, A.R., Solouki, M., Omidi, M., Irvani, N., Nezad, M. Mahdi and Rezazadeh, S.H. (2010). Callus induction and plant regeneration in Ferula assafoetida L. (Asafetida), an endangered medicinal plant. Trakia J. Sci., 8: 11-18.

  • Zhu, J., Li, X.J., Kaisa, S. and Jia, X.G. (2009). Study on callus induction of Ferula sinkiangensis. Zhong Yao.


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  • Callus formation in Ferula jaeschkeana Vatke

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Authors

R. K. Sharma
Department of Botany, Government College for Women, Gandhi Nagar, Jammu (J&K), India

Abstract


Callus was initiated from the petiole explants of Ferula jaeschakeana on a medium containing 2,4-D. The highest percentage of explants forming callus (92.5) and callus index (258) were obtained on medium supplemented with 1 mgl-1 2,4-D. Among explants sizes and type tested for callus initiation, 10 mm long petiole gave maximum callus index. Small and large explants initiated a slight amount of callus. Addition of Kn in combination with 2,4-D enhanced the callus formation from the explants.

Keywords


Callus, Ferula jaeschkeana, Tissue Culture.

References