Open Access
Subscription Access
Open Access
Subscription Access
Ultraviolet A (UV-A) Radiation-Induced Damage in the Skin and Vital Organs of Albino Rat: An Indirect Correlation with Melatonin
Subscribe/Renew Journal
Ultraviolet radiation is causative of generation of reactive oxygen species (ROS) in the body that significantly affects normal physiology and disturbs homeostasis. In the present study we investigated the effect of UV-A radiation exposure on the first line of defence system such as skin and vital organs such as liver, kidney and spleen of Rattus norvegicus. Adult female rats were exposed to UV-A radiation for seven days at a dose of 6.36 J/cm2 and the changes in the skin histoarchitecture, oxidative load of spleen, liver and kidney along with cellular ROS levels of splenocyte determined using DCFDA staining were recorded. UV-A exposure severely damaged the histoarchitecture of skin and reduced proliferating cell nuclear antigen (PCNA) expression. The lipid peroxidation (MDA) level in spleen, liver and kidney were increased to significant levels while the activities of the enzymatic antioxidants, SOD and catalase were significantly decreased. Significant decrease of glucose content and increase of LDH of both spleen and liver were found. Cellular damage of splenocyte was observed as evidenced by increase in percentage of intense DCFDA-stained cells in UV-A treated rats. Thus, our results clearly demonstrate that UV-A radiation exposure may have detrimental effects on the antioxidant defence system of the body, including melatonin, leading to disruption of physiology by affecting vital organs.
Keywords
Kidney, Liver, Oxidative Stress; Skin, Spleen, Splenocyte, DCFDA, PCNA, UV-A Radiation.
Subscription
Login to verify subscription
User
Font Size
Information
- Valencia A, Rajadurai A, Carle AB, Kochevar IE. 7-Dehydrocholesterol enhances ultraviolet A-induced oxidative stress in keratinocytes: roles of NADPH oxidase, mitochondria and lipid rafts. Free Radic Biol Med. 2006; 41: 1704–1718. https:// doi.org/10.1016/j.freeradbiomed.2006.09.006 PMid:17145559 PMCid:PMC1880892
- Douki T, Reynaud-Angelin, A, Cade, J, Sage E. Bipyrimidine photoproducts rather than oxidative lesions are the main type of DNA damage involved in the genotoxic effect of solar UVA radiation. Biochemistry. 2003; 42: 9221–9226. https://doi.org/10.1021/bi034593c PMid:12885257
- Halliday, G. M. Inflammation, gene mutation and photoimmunosuppression in response to UVR-induced oxidative damage contributes to photocarcinogenesis. Mutat. Res. 2005; 571: 107–120. https://doi.org/10.1016/j.mrfmmm.2004.09.013 PMid:15748642
- Goswami, S, Haldar, C. Melatonin pre-treatment alleviates UVA radiation induced oxidative stress and apoptosis in the skin of a diurnal tropical rodent Funambulus pennantii. J Nucl Med Radiat Ther. 2016; 8: 318.
- Verma R, Haldar C. Photoperiodic modulation of thyroid hormone receptor (TR-α), deiodinase-2 (Dio-2) and glucose transporters (GLUT 1 and GLUT 4) expression in testis of adult golden hamster, Mesocricetus auratus. J Photochem Photobiol B. 2016; 165: 351–358. https://doi.org/10.1016/j.jphotobiol.2016.10.036 PMid:27838488
- Bradford, MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal Biochem. 1976; 72: 248–254. https://doi.org/10.1016/0003-2697(76)90527-3
- Vishwas DK, Mukherjee A, Haldar C, Dash D, and Nayak MK. Improvement of oxidative stress and immunity by melatonin: an age dependent study in golden hamster. Exp Gerontol. 2013; 48: 168–182. https://doi.org/10.1016/j.exger.2012.11.012 https://doi.org/10.1016/j.exger.2013.05.052 PMid:23220117
- Lijun L, Xuemei L, Yaping G, EnboM. Activity of the enzymes of the antioxidative system in cadmium-treated Oxya chinensi (Orthoptera Acridoidae). Environ Toxicol Phar. 2005; 20: 412–416. https://doi.org/10.1016/j.etap.2005.04.001 PMid:21783620
- McMillan TJ, Leatherman E, Ridley A, Shorrocks J, Tobi SE, Whiteside JR. Cellular effects of long wavelength UV light (UVA) in mammalian cells. J Pharm Pharmacol. 2008; 60: 969– 976. https://doi.org/10.1211/jpp.60.8.0004 PMid:18644190
- Hasei K, Ichihashi M. Solar urticaria: determinations of action and inhibition spectra Arch. Arch of Dermatol. 1982; 118: 346–350. https://doi.org/10.1001/archderm.118.5.346 https://doi.org/10.1001/archderm.1982.01650170060026
- Ortel B, Tanew A, Wolff K, Honisgman H. Polymorphous light eruption: action spectrum and photoprotection. J Am Acad Dermatol. 1986; 14: 748–753. https://doi.org/10.1016/S0190-9622(86)70088-1
- Garland CF, Garland FC, Gorham EC. Epidemiologic evidence for different roles of ultraviolet A and B radiation in melanoma mortality rates. Ann Epidemiol (AEP). 2003; 13: 395–404. https://doi.org/10.1016/S1047-2797(02)00461-1
- Agar NS, Halliday GM, Barnetson RStC, et al. The basal layer in human squamous tumors harbors more UVA than UVB fingerprint mutations: a role for UVA in human skin carcinogenesis. Proc Natl Acad Sci USA. 2004; 101: 4954–4959. https://doi.org/10.1073/pnas.0401141101 PMid:15041750 PMCid:PMC387355
Abstract Views: 324
PDF Views: 0