Journal of Endocrinology and Reproduction

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Rapid In Situ Action of Estradiol 17β on Ion Transporter Function in Brain Segments of Female Mozambique Tilapia (Oreochromis mossambicus Peters)


Affiliations
1 Department of Zoology, University of Kerala, Kariavattom, Thiruvananthapuram – 695581, Kerala, India
2 Inter-University Centre for Evolutionary and Integrative Biology, University of Kerala, Kariavattom, Thiruvananthapuram – 695581, Kerala, India
     

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Being the principal estrogen, estradiol 17β (E2) is essential for normal ovarian function in the vertebrates including fishes. Besides its primary role in reproduction, E2 is also known for its role in many other physiological processes including water and mineral balance. However, it is uncertain, how E2 regulates ion-specific ATPases that drive Na+, K+, H+, Ca2+ and Mg2+ transport in fish brain. We, therefore, examined the short-term in situ action of E2 on ion transporter function in the brain segments of freshwater female Mozambique tilapia Oreochromis mossambicus. Tilapia were perfused with increasing doses of E2 (10-9, 10-8 and 10-7 M) for 20 min and sampled for determining Na+/K+-ATPase, H+-ATPase, Ca2+-ATPase, and Mg2+-ATPase activities in the prosencephalon (PC), mesencephalon (MC) and metencephalon (MeC) segments of brain. Dose-dependent increase in Na+/K+- and Ca2+-dependent transporter activities after E2 perfusion were found in PC. In MC, E2 treatment, however, produced significant increase in Mg2+, Ca2+ and H+ transport activities in mitochondria but decreased Na+/K+- and νH+ transporter activities. On the contrary, in MeC, E2 administration while producing increase in Na+/K+-, mitochondrial- and νH+-transport, lowered cytosolic and mitochondrial Ca2+ transport. Taken together, the data indicate that E2 has rapid and direct action on ion transporter function that corresponds to the differential activation/inactivation of neuronal clusters in the brain segments of female freshwater tilapia.

Keywords

Na+/K+-ATPase, Estradiol 17β, Fish, Ion Transporter, Ionoregulation, Tilapia brain.
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  • Rapid In Situ Action of Estradiol 17β on Ion Transporter Function in Brain Segments of Female Mozambique Tilapia (Oreochromis mossambicus Peters)

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Authors

Dafina Wilfred
Department of Zoology, University of Kerala, Kariavattom, Thiruvananthapuram – 695581, Kerala, India
Valsa S. Peter
Inter-University Centre for Evolutionary and Integrative Biology, University of Kerala, Kariavattom, Thiruvananthapuram – 695581, Kerala, India
Subash Peter
Department of Zoology, University of Kerala, Kariavattom, Thiruvananthapuram – 695581, Kerala, India

Abstract


Being the principal estrogen, estradiol 17β (E2) is essential for normal ovarian function in the vertebrates including fishes. Besides its primary role in reproduction, E2 is also known for its role in many other physiological processes including water and mineral balance. However, it is uncertain, how E2 regulates ion-specific ATPases that drive Na+, K+, H+, Ca2+ and Mg2+ transport in fish brain. We, therefore, examined the short-term in situ action of E2 on ion transporter function in the brain segments of freshwater female Mozambique tilapia Oreochromis mossambicus. Tilapia were perfused with increasing doses of E2 (10-9, 10-8 and 10-7 M) for 20 min and sampled for determining Na+/K+-ATPase, H+-ATPase, Ca2+-ATPase, and Mg2+-ATPase activities in the prosencephalon (PC), mesencephalon (MC) and metencephalon (MeC) segments of brain. Dose-dependent increase in Na+/K+- and Ca2+-dependent transporter activities after E2 perfusion were found in PC. In MC, E2 treatment, however, produced significant increase in Mg2+, Ca2+ and H+ transport activities in mitochondria but decreased Na+/K+- and νH+ transporter activities. On the contrary, in MeC, E2 administration while producing increase in Na+/K+-, mitochondrial- and νH+-transport, lowered cytosolic and mitochondrial Ca2+ transport. Taken together, the data indicate that E2 has rapid and direct action on ion transporter function that corresponds to the differential activation/inactivation of neuronal clusters in the brain segments of female freshwater tilapia.

Keywords


Na+/K+-ATPase, Estradiol 17β, Fish, Ion Transporter, Ionoregulation, Tilapia brain.

References





DOI: https://doi.org/10.18311/jer%2F2017%2F23150