Modulatory Effects of Centrophenoxine on Salivary Glands in D-Galactose Induced Aged Female Mice

Three month old female mice were ovariectomised and after 4 days the mice were separated into six groups, I) ovariectomized, II) ovariectomized + D-galactose treated, III) ovariectomized + D-galactose treated + Tween 80, IV) ovariectomized + D-galactose&centrophenoxine treated, V) ovariectomized D-galactose treated + olive oil injected, and VI) ovariectomized + D-galactose treated + estrogen injected. After 15 days the mice were sacrificed. The protein concentration, amylase activity, lipid peroxidation and fluorescence product were measured in various salivary glands, viz., submandibular, sublingual, and parotid. In ovariectomized (group II) and ovariectomized + D-galactose treated (group III) mice the protein concentration and amylase activity of various salivary glands decreased, while lipid peroxidation and fluorescence product increased. In ovariectomized + D-galactose + centrophenoxine treated (group IV) and ovariectomized + D-galactose treated + estrogen injected (group VI) mice protein concentration and amylase activity in the salivary glands increased, while lipid peroxidation and fluorescence product decreased. These results reveal that centrophenoxine, and therefore aging, has modulatory effects on salivary glands in D-galactose induced aged female mice.