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4(R)-5, 10, Seco 19-Norpregna 4,5 Diene 3, 10, 20 Trione (Seco), an Inhibitor of Epididymal 5α-Reductase Arrests Sperm Forward Motility in Wistar Rats


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1 Department of Endocrinology, Dr. ALM. PG. Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600 113, India
     

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The effect of 4(R) - 5, 10, Seco 19 - norpregna 4,5 diene 3,10, 20 trione (SECO), a potent inhibitor of 5α-reductase, an intracellular enzyme that converts testosterone to dihydrotestosterone (DHT), on epididymal sperm count and motility was studied in Wistar rats. Administration of (i.m.) 250, 500, 750,1000 μg SECO/Kg b.wt/day to mature rats for 45 days impaired sperm forward motility in a dose dependent manner. Epididymal weight and sperm number decreased in rats treated with 750 and 1000 μg SECO, whereas weight of other accessory organs decreased in rats treated with 1000 μg SECO only. The epididymal histology showed degraded sperm and increased number of clear cells indicating high endocytotic activity, to clear dead sperm, whereas no obvious change was observed in the testicular histology. SECO treatment increased testosterone concentration whereas, it decreased estradiol concentration in circulation and in the epididymal tissue. SECO treatment specifically decreased 5α-reductase in epididymis without affecting the same in the liver, indicating its targeted effect on the epididymis. Thus, the preliminary in vivo study points out that SECO impairs the acquisition of sperm forward motility.

Keywords

Epididymis, 5α-Reductase Inhibitor, SECO, Sperm Fordward Motility.
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  • 4(R)-5, 10, Seco 19-Norpregna 4,5 Diene 3, 10, 20 Trione (Seco), an Inhibitor of Epididymal 5α-Reductase Arrests Sperm Forward Motility in Wistar Rats

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Authors

M. Michael Aruldhas
Department of Endocrinology, Dr. ALM. PG. Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600 113, India
G. Rajendiran
Department of Endocrinology, Dr. ALM. PG. Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600 113, India
S. Subramanian
Department of Endocrinology, Dr. ALM. PG. Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600 113, India
S. Dharmalingam
Department of Endocrinology, Dr. ALM. PG. Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600 113, India
P. Govindarajulu
Department of Endocrinology, Dr. ALM. PG. Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600 113, India

Abstract


The effect of 4(R) - 5, 10, Seco 19 - norpregna 4,5 diene 3,10, 20 trione (SECO), a potent inhibitor of 5α-reductase, an intracellular enzyme that converts testosterone to dihydrotestosterone (DHT), on epididymal sperm count and motility was studied in Wistar rats. Administration of (i.m.) 250, 500, 750,1000 μg SECO/Kg b.wt/day to mature rats for 45 days impaired sperm forward motility in a dose dependent manner. Epididymal weight and sperm number decreased in rats treated with 750 and 1000 μg SECO, whereas weight of other accessory organs decreased in rats treated with 1000 μg SECO only. The epididymal histology showed degraded sperm and increased number of clear cells indicating high endocytotic activity, to clear dead sperm, whereas no obvious change was observed in the testicular histology. SECO treatment increased testosterone concentration whereas, it decreased estradiol concentration in circulation and in the epididymal tissue. SECO treatment specifically decreased 5α-reductase in epididymis without affecting the same in the liver, indicating its targeted effect on the epididymis. Thus, the preliminary in vivo study points out that SECO impairs the acquisition of sperm forward motility.

Keywords


Epididymis, 5α-Reductase Inhibitor, SECO, Sperm Fordward Motility.



DOI: https://doi.org/10.18519/jer%2F1999%2Fv3%2F98661