Journal of Endocrinology and Reproduction

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Involvement of cAMP in Basal and Estradiol-17β(E2) Modulated Activity of Brain Tyrosine Hydroxylase in the Female Catfish Heteropneustes fossilis:An in vitro Study


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1 Department of Zoology, Banaras Hindu University, Varanasi 221 005, India
     

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In the present in vitro study, involvement of cAMP in basal and E2-modulated brain (hypothalamus and telencephalon) TH activity was demonstrated in female catfish Heteropneustes fossilis. Incubation of enzyme preparation (soluble active fraction) with cAMP (0.05-1 .OmM) and inhibitors of cAMP-specific phosphodiesterase (IBMX, 0.5-2.0mM) and theophylline, 0.5-2.0mM) stimulated enzyme activity in a concentration-dependent manner.
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  • Involvement of cAMP in Basal and Estradiol-17β(E2) Modulated Activity of Brain Tyrosine Hydroxylase in the Female Catfish Heteropneustes fossilis:An in vitro Study

Abstract Views: 276  |  PDF Views: 0

Authors

R. Chaube
Department of Zoology, Banaras Hindu University, Varanasi 221 005, India
K. P. Joy
Department of Zoology, Banaras Hindu University, Varanasi 221 005, India

Abstract


In the present in vitro study, involvement of cAMP in basal and E2-modulated brain (hypothalamus and telencephalon) TH activity was demonstrated in female catfish Heteropneustes fossilis. Incubation of enzyme preparation (soluble active fraction) with cAMP (0.05-1 .OmM) and inhibitors of cAMP-specific phosphodiesterase (IBMX, 0.5-2.0mM) and theophylline, 0.5-2.0mM) stimulated enzyme activity in a concentration-dependent manner.


DOI: https://doi.org/10.18519/jer%2F2003%2Fv7%2F98798