Journal of Environment and Sociobiology

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Melissopalynological Studies on Multifloral Honeys from Arambagh Region of Hooghly District, West Bengal


Affiliations
1 Department of Botany, Asutosh College, 92, S.P. Mukherjee Road, Kolkata-700026, West Bengal, India
     

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Pollen grains are important constituent of honey and are collected by the honey bees during forage to blossoms. The characterization and identification of pollen grains from honey samples are of great importance for its quantitative and qualitative assessment. For melissopalynological analysis the honey samples were collected from the investigated regions. The collected samples were acetolysed and pollen grains were studied by optical microscopy. The flowering vegetation of this region was surveyed and the flowering period was recorded. The honey samples investigated are of multifloral sources. Twenty three dominant pollen morphotypes were identified from the honey samples. The identified pollen morphotypes are of Acacia nilotica, Alstonia scholaris, Anisomeles indica, Azadirachta indica, Blumea lacera, Borassus flabellifer, Brassica campestris, Butea monosperma, Carica papaya, Chenopodium album, Eucalyptus citriodora, Hygrophila phlomoides, Litchi chinensis, Mangifera indica, Moringa oleifera, Murraya paniculata, Ocimum canum, Pongamia pinnata, Salvia sp., Sesamum indicum, Syzygium cumini, Thevetia peruviana and Zizyphus mauritiana. The bee specimens identified form the hives were Apis dorsata, Apis cerana indica and Apis mellifera. The pollen grains are mostly tricolporate with reticulate exine ornamentation. The aim of this study is to establish the plant species flowering in the foraging area with the pollen morphotypes identified from honey samples.

Keywords

Melissopalynology, Multifloral Honey, Honey Bee, Pollen Morphotypes.
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  • Adeonipekun, P. A., Adeniyi, T. A. and Eden, D. 2016. Antimicrobial properties and melissopalynology, proximate and elemental analyses of honey samples from three different ecozones in Nigeria. Notulac Scientia Biologicae, 8(3): 326-333; doi:10.15835/nsb.8.3.9844.

  • Aronne, G. and Micco, V. De. 2010. Traditional melissopalynology integrated by multivariate analysis and sampling methods to improve botanical and geographical characterization of honeys. Plant Biosystems, 144(4): 833-840.

  • Barber, D., Diaz-Perales, A., Villalba, M. and Chivato, T. 2015. Challenges for allergy diagnosis in regions with complex pollen exposures. Curr. Allergy Asthma Rep., 15(496): 2-10.

  • Bauma, K. A., Rubink, W. L., Coulson, R. N. and Bryant, J. R. V. M. 2011. Diurnal patterns of pollen collection by feral honey bee colonies in southern Texas, USA. Palynology, 35(1): 85-93.

  • Chakraborty, P., Gupta-Bhattacharya, S., Roy, I. and Chanda, S. 2004. Identification of shared allergic components from four common and dominant pollen taxa of Arecaceae. Current Science, 86(11): 1539-1543.

  • Chanda, S. and Gupta-Bhattacharya, S. 2003. Biological air pollution - a case study in Salt Lake City, Calcutta, India. In: Environmental Issues for the 21st Century (Eds.) by Das-Gupta, S. P. Mittal Publications, New Delhi : 55-70.

  • Chaturvedi, S. K. and Temsunungla. 2009. Melissopalynological studies at Kupza village of Mokokchung district, Nagaland. Journal of Palynology, 43(1-2): 89-100.

  • Demoly, P., Calderon, M. A., Casale T. B., Malling, H. and Wahn, U. 2015. The value of pre-and co-seasonal sublingual immunotherapy in pollen-induced allergic rhinoconjunctivitis. Clinical and Translational Allergy, 5(18): 1-10.

  • Deodikar, B. B. and Thakar, C. U. 1953. A pollen theory of major honey yielding plants of Mahabaleswar Hills. Apicultural Laboratory, Bull. No. 1, p. 8.

  • Didier, A., Wahn, U., Horak, F. and Cox, L. S. 2014. Five-grass-pollen sublingual immunotherapy tablet for the treatment of grass-pollen-induced allergic rhinoconjunctivitis: 5 years of experience. Expert Rev. Clin. Immunol., 10: 1309-1324.

  • Dimou, M. and Thrasyvoulou, A. 2009. Pollen analysis of honeybee rectum as a method to record the bee pollen flora of an area. Apidologie, 40(2): 124-133.

  • Dogan, C. and Sorkum, K. 2001. Pollen analyses of honey from Aegean, Marmara, Mediterranean and Black Sea regions of Turkey. Mellifera, 1: 2-12.

  • Erdtman, G. (1960). The acetolysis method. A revised description. Svensk Botanisk Tidskrift, 54: 561-564.

  • Ganguly, P., Gupta, S., Bhattacharya, K. and Chanda, S. 1984. Comparative pollen spectra of honey samples from West Dinajpur and Jalpaiguri districts of West Bengal. Science and Culure, 50: 170-172.

  • Giorgi, A., Madeo, M., Baumgartner, J. and Lozzia, G. C. 2011. The relationship between phenolic content, pollen diversity, physicochemical information and radical scavenging activity in honey. Molecules, 16: 336-347; doi: 10.3390/molecules 16010336.

  • Hao, G. D., Zheng, Y. W., Gjesing, B., Kong, X. A., Wang, J. Y., Song, Z. J. et al. 2013. Prevalence of sensitization to weed pollens of Humulus scandens, Artemisia vulgaris, and Ambrosia artemisiifolia in northern China. J. Zhejiang. Univ. Sci. B.,14: 240-246.

  • Jantakee, K. and Tragoolpua, Y. 2015. Activities of different types of Thai honey on pathogenic bacteria causing skin diseases, tyrosinase enzyme and generating free radicals. Biological Research, 48(4): 1-11.

  • Karagiannis, E., Shah-Hosseini, K., Hadler, M. and Mosges, R. 2014. PD14-Noninterventional 2-year study of sublingual immunotherapy in children and adolescents with allergic rhinoconjunctivitis caused by grass pollen. Clin. Trans. Aller., 4 (Suppl 1): 14.

  • Kaur, A. and Mattu, V. K. (2016). Pollen spectrum of honey samples of Apis cerana F. collected from different areas of Shiwalik hills. International Journal of Scientific and Education,4(6): 5434-5440; doi:http://dx.doi.org/10.18535/ijsre/v4io6.01.

  • Kaya, Z., Binzet, R. and Orcan, N. 2005. Pollen analysis of honey from some regions in Turkey. Apiacta, 40: 10-15.

  • Kazuhiko, I., Weinberger, K. R., Robinson, G. S., Sheffield, P. E., Lall, R., Mathes, R., Ross, Z., Kinney, P. L. and Matte, T. D. 2015. The associations between daily spring pollen counts, over-the-counter allergy medication sales, and asthma syndrome emergency department visits in New York City, 2002-2012. Environmental Health, 14(71): 1-12.

  • Lazarova, M. A., Atanassova, J. R. and Yurukova, L. D. 2010. Botanical origin and inorganic content of bee honey in Northeast Bulgaria (Shumen region). Phytologia Balcanica, 16(1): 131-135.

  • Lieux, M. H. 1972. Melissopalynological study of 54 Louisianan (USA) honeys. Review of Paleobotany and Palynology, 13: 95-124.

  • Lieux, M. H. 1975. Dominant pollen types recovered from commercial Louisiana honeys. Economic Botany, 29: 78-96.

  • Louveaux, J., Maurizio, A. and Vorwohl, G. 1978. Methods of melissopalynology. International Bee Research Association. Bee World, 59(4): 139-157.

  • Mandal, S. and De, A. K. 1988. Melissopalynological study of honey samples of Birbhum district, West Bengal. Indian Biologist, 20: 8-12.

  • Mandal, S. 1989. Melissopalynological study of honey sample of Murshidabad district, West Bengal. Science and Culture, 55: 178-181.

  • Moar, N. T. 1985. Pollen analysis of Newzealand honey. Journal of Apicultural Research, 28: 39-70.

  • Mondal, M. and Mitra, K. 1980. Pollen analysis of honey from Sundarbans (West Bengal). Geophytology, 10(2): 137-139.

  • Romas, S. E., Perez, B. M. and Ferreros, G. C. 1999. Pollen characterization of multifloral honeys from La Parma (Canary Island). Grana, 38: 356-360.

  • Sanchez-Mesa, J. A., Serrano, P., Carinanos, P., Prieto- Baena, J. C., Moreno, C., Guerra, F. and Galan, C. 2005. Pollen allergy in Cordoba city: frequency of sensitization and relation with antihistamine sales. Journal of Investigational and Allergology Clinical Immunology, 15(1): 50-56.

  • Singh, A. B. and Kumar, P. 2003. Aeroallergens in clinical practice of allergy in India. An overview. Annals of Agricultural and Environmental Medicine, 10: 131-136.

  • Singh, S. and Kar, R. 2011. Melissopalynological studies on mangrove honeys from Sunderban (Bangladesh) and Little Andaman (India). Current Science, 100 (9): 1290-1293.

  • Valencial, R. M., Horrera, B. and Molnar, T. (2000). Pollen and organoleptic analysis of honeys in Leon Province (Spain). Grana, 39: 133-140.

  • Westerhout, K. Y., Verheggen, B. G., Schreder, C. H. and Augustin, M. 2012. Cost effectiveness analysis of immunotherapy in patients with grass pollen allergic rhinoconjunctivitis in Germany. J. Med. Econ., 15: 906-917.


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  • Melissopalynological Studies on Multifloral Honeys from Arambagh Region of Hooghly District, West Bengal

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Authors

Pradyut Biswas
Department of Botany, Asutosh College, 92, S.P. Mukherjee Road, Kolkata-700026, West Bengal, India

Abstract


Pollen grains are important constituent of honey and are collected by the honey bees during forage to blossoms. The characterization and identification of pollen grains from honey samples are of great importance for its quantitative and qualitative assessment. For melissopalynological analysis the honey samples were collected from the investigated regions. The collected samples were acetolysed and pollen grains were studied by optical microscopy. The flowering vegetation of this region was surveyed and the flowering period was recorded. The honey samples investigated are of multifloral sources. Twenty three dominant pollen morphotypes were identified from the honey samples. The identified pollen morphotypes are of Acacia nilotica, Alstonia scholaris, Anisomeles indica, Azadirachta indica, Blumea lacera, Borassus flabellifer, Brassica campestris, Butea monosperma, Carica papaya, Chenopodium album, Eucalyptus citriodora, Hygrophila phlomoides, Litchi chinensis, Mangifera indica, Moringa oleifera, Murraya paniculata, Ocimum canum, Pongamia pinnata, Salvia sp., Sesamum indicum, Syzygium cumini, Thevetia peruviana and Zizyphus mauritiana. The bee specimens identified form the hives were Apis dorsata, Apis cerana indica and Apis mellifera. The pollen grains are mostly tricolporate with reticulate exine ornamentation. The aim of this study is to establish the plant species flowering in the foraging area with the pollen morphotypes identified from honey samples.

Keywords


Melissopalynology, Multifloral Honey, Honey Bee, Pollen Morphotypes.

References