Journal of Environment and Sociobiology

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Assessment of the Relationship of Micronuclei, Anomalous Nuclei and Nucleo-Cytoplasmic Ratio of Red Blood Corpuscles in Fish Induced by Ethyl Methane Sulphonate


Affiliations
1 Department of Zoology, Netaji Subhas Open University, DD-26, Sector-1, Salt Lake City, Kolkata-700 064, India
     

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Two different doses (1% and 2%) of Ethyl Methane Sulphonate (EMS), a known mutagen was treated on two different fishes, namely, tilapia (Oreochromis mossambicus) and koi (Anabas testudineus) in respect of suitable distilled water treated control. The present paper aims to assess the relationship of micronuclei, anomalous nuclei and nucleo-cytoplasmic ratio of red blood corpuscles induced by EMS in these fish models. The result shows a positive co-relation among all these parameters examined and inflict an insight towards the relevance of these parameters on the pollution status of the water body.

Keywords

Mutagen, Nuclear abnormalities, Cytotoxicity, Genotoxicity, Erythrocytes, Aquatic toxicity, Fish.
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  • Basu, S. and Guha, B. 2012. Assessment of genotoxicity induced by the effluent of supreme paper mill (India) on the fish Heteropneustes fossilis (Bloch). Proc. Zool. Soc., 65(2): 86-90.

  • Carrasco, K. R., Tilbury, K. L. and Myers, M. S. (1990). Assessment of piscine micronucleus test as an in situ biological indicator of chemical contaminated effects. Can. J. Fish. & Aquat. Sci., 47: 2123-2136.

  • Cavas, T. and Ergene-Gozukara, S. 2003. Micronuclei, nuclear lesions and interphase silver-stained nucleolar organizer regions (AgNORs) as cyto-genotoxicity indicators in Oreochromis niloticus exposed to textile mill effluent. Mutation Research, 534: 93-99.

  • Cavas, C. T. and Ergene-Gozukara, S. 2005. Induction of micronuclei and nuclear abnormalities in Oreochromis niloticus following exposure to petroleum refinery and chromium processing plant effluents. Aquatic Toxicology, 74: 264-271.

  • Cavas, T. Garanko, N. N. and Arkhipchuk, V. V. (2005). Induction of micronuclei and binuclei in blood, gill and liver cells of shessubchronically exposed to cadmium chloride and copper sulphate. Food Chemistry Toxicology, 43: 569-574.

  • Cohn, N. S. (1979). Elements of Cytology, 2ndedn, Freeman Book Co, Delhi, India.

  • Fenech, M. and Crott, J. W. 2002. Micronuclei, nucleoplasmic bridges and nuclear buds induced in folic acid deficient human lymphocytes -evidence for breakagefusionbridge cycles in the cytokinesis block micronucleus assay. Mutation Research, 504: 131-136.

  • Fisher, R. A. and Yates, F. 1963. Statistical Tables for Biological, Agricultural and Medical Research, Sixth ed. Oliver and Boyd, Edinburgh.

  • Grisolia, C. K., Rivero, C. L. G., Starling, F. L. R. M., Silva, I. C. R., Barbosa, A. C. and Dorea, J. G. 2009. Profile of micronucleus frequencies and DNA damage in different species of fish in a eutrophic tropical lake. Genet Mol. Biol., 32: 138-143.

  • Guha, B. and Khuda-Bukhsh, A. R. 2002. Efficacy of vitamin-C (L-ascorbic acid) in reducing genotoxicity in fish (Oreochromis mossambicus) induced by ethyl methane sulphonate. Chemosphere, 47: 49-56.

  • Guha, B. and Khuda-Bukhsh, A. R. 2003. Ameliorating effect of β-carotene on ethyl methane sulphonate (EMS) induced genotoxicity in a fish Oreochromis mossambicus. Mutation Research, 542(1-2): 1-13.

  • Guha, B., Das, J. K. and Khuda-Bukhsh, A. R. 2007. Ameliorative effects of vitamin supplementation on ethyl methane sulphonate-induced genotoxicity in a fish, Anabas testudineus. Ecotoxicology and Environmental Safety, 68(1): 63-70.

  • Heddle, J. A. 1973. A rapid in vivo test for chromosomal damage. Mutation Research, 18: 307-317.

  • Heddle, J. A., Cimino, M. C., Hayashi, M., Romagna, F., Shelby, M. D., Tucker, J. D., Vanprays, P. and MacGregor, J. T. 1991. Micronuclei as an index of cytogenetic damage: past, present and future. Environmental and Molecular Mutagenesis, 18: 277-291.

  • Hooftman, R. N. and Vink, G. J. 1981. Cytogenetic effects on the eastern mudminnow, Umbra pygmaea, exposed to ethyl methane sulphonate, benzo [a] pyrene and river water. Ecotoxicology and Environmental Safety, 5: 261-269.

  • Hooftman, R. N. and De Raat, W. K. (1982). Induction of nuclear anomalies (micronuclei) in the peripheral blood erythrocytes of Eastern mudminnow Umbra pygmaea by ethyl methane sulphonate. Mutation Research, 104: 147– 152.

  • Khuda-Bukhsh, A. R., Chakraborti, J., Mallick, P. and Mohanty, K. C. 2000. Genotoxic studies in natural populations of fish as an indicator of aquatic pollution in Hooghly-Matlah estuary system. Proc. Intl. Conf. Ecol. Eng. Nov 23-27, 1998, Science City, Calcutta. In: Waste Recycling and Resource Management in the Developing World, (Eds Jana BB, Banerjee RD, Guterstam B & Heeb J) pp. 377-385.

  • Kihlman, B. A. 1966. Action of chemicals on dividing cells. Prentice Hall, Eaglewood.

  • Kligerman, A. D. 1982. Fishes as biological detectors of the effects of genotoxic agents. In: Mutagenicity New Horizons in Genetic Toxicology (Ed. Heddle JH), Academic Press, New York, pp. 435-457.

  • Malacinski, G. M. and Freifdelder, D. (1988). Mutation, mutagenesis and DNA repair, In: Essentials of molecular Biology (3rd ed.), Jones and Bartlet Publishers Internationals, UK, pp. 213-247.

  • Manna, G. K. 1984. Progress in fish cytogenetics. Nucleus, 27: 203-247.

  • Manna, G. K. (1986). Tilapia fish as a model for testing genotoxic agents. In: Perspectives in Cytology and Genetics, (Eds. Manna, G. K. and Sinha U), 6: 47-61.

  • Manna, G. K. and Biswas, S. 1986. Micronucleus test (MNT) in four species of fishes treated with the bacterium, Pseudomonas aeruginosa. National Academy of Science Letters, 9: 189-191.

  • Manna, G. K. and Som, R. C. 1982. Effect of X-rays on the somatic chromosomes of the exotic fish, Tilapia mossambica. Proc. Indian Acad. Sci. (Anim. Sci.), 91: 121-133.

  • Manna, G. K. and Mukherjee, R. K. 1989. A study of the genotoxic potentiality of the inorganic weedicide, sodium arsenite in the experimentally treated tilapia fish. Journal of Freshwater Biology, 1: 147-159.

  • Manna, G. K., Banerjee, G. and Gupta, S. 1985. Micronucleus test of different genotoxic agents in peripheral erythrocytes of the exotic fish, Sarotherodon mosambica. Nucleus, 2: 176-179.

  • Porichha, S. K., Sarangi, P. K. and Prasad, R. (1998). Cytogenetic effect of chlorpyriphos in Chhana punctatus. In: Proc IXth All India Cong Cytol Genet, Chandigarh, Nov 22-25, 1996, In: Pers Cytol Genet (Eds Manna GK & Roy Sc), 9: 631-638.

  • Prien, A. E., Thie, G. M., Alink, G. M., Poets, C. L. M. and Koeman, J. H. 1977. Cytogenetic changes in fish exposed to water of river Rhine. Science of the Total Environment, 9: 287-291.

  • Schmid, W. 1976. The micronucleus test for cytogenetic analysis. In: A. Hollaender (ed.), Chemical Mutagens, Vol. 4, Plenum, New York, pp. 31-53.


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  • Assessment of the Relationship of Micronuclei, Anomalous Nuclei and Nucleo-Cytoplasmic Ratio of Red Blood Corpuscles in Fish Induced by Ethyl Methane Sulphonate

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Authors

Onkar Nath Ghosh
Department of Zoology, Netaji Subhas Open University, DD-26, Sector-1, Salt Lake City, Kolkata-700 064, India
Bibhas Guha
Department of Zoology, Netaji Subhas Open University, DD-26, Sector-1, Salt Lake City, Kolkata-700 064, India

Abstract


Two different doses (1% and 2%) of Ethyl Methane Sulphonate (EMS), a known mutagen was treated on two different fishes, namely, tilapia (Oreochromis mossambicus) and koi (Anabas testudineus) in respect of suitable distilled water treated control. The present paper aims to assess the relationship of micronuclei, anomalous nuclei and nucleo-cytoplasmic ratio of red blood corpuscles induced by EMS in these fish models. The result shows a positive co-relation among all these parameters examined and inflict an insight towards the relevance of these parameters on the pollution status of the water body.

Keywords


Mutagen, Nuclear abnormalities, Cytotoxicity, Genotoxicity, Erythrocytes, Aquatic toxicity, Fish.

References