Journal of Environment and Sociobiology

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Analysis of Genetic Diversity of Protoplast Fused Mushrooms Pleurotus Florida X Lentinus Squorrosulus (PFLS) Somatic Hybrids through Rapd Markers


Affiliations
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, India
2 Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, India
     

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A total of twelve somatic hybrid strains, Pleurotus florida x Lentinus squorrosulus (pfls), were characterized through PCR based RAPD analysis. Sixteen random decemer primers yielded a total of 136 fragments with 97.79 % polymorphism with an amplicon range of 200 bp - 3000 bp in size. RAPD-08 showed optimum amplification and generated 12 scorable bands (250 bp -1800 bp) where RAPD-07 amplified least and generated 5 scorable bands (400 bp - 1300 bp). PIC ranged from 0.424 (SRS-15) to 0.499 (SRS-16). Based on Jaccard’s proximity matrix the highest genetic distance was found in between parent L. squarrosulus and hybrid pfls1q which means they are genetically most distantly related. Among the hybrid pfls1k and pfls1p showed maximum genetic distance while the most closely related hybrids were pfls1i and pfls1l. The parent L. squarrosulus was most genetically distant individual than the other parent P. florida.

Keywords

Hybrid Mushroom, Genetic Diversity, Polymorphism, RAPD.
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  • Analysis of Genetic Diversity of Protoplast Fused Mushrooms Pleurotus Florida X Lentinus Squorrosulus (PFLS) Somatic Hybrids through Rapd Markers

Abstract Views: 282  |  PDF Views: 1

Authors

Neelanjana Bag
Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, India
Bijay Pal
Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, India
Samir Ranjan Sikdar
Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, India
Pijush Mallick
Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, India

Abstract


A total of twelve somatic hybrid strains, Pleurotus florida x Lentinus squorrosulus (pfls), were characterized through PCR based RAPD analysis. Sixteen random decemer primers yielded a total of 136 fragments with 97.79 % polymorphism with an amplicon range of 200 bp - 3000 bp in size. RAPD-08 showed optimum amplification and generated 12 scorable bands (250 bp -1800 bp) where RAPD-07 amplified least and generated 5 scorable bands (400 bp - 1300 bp). PIC ranged from 0.424 (SRS-15) to 0.499 (SRS-16). Based on Jaccard’s proximity matrix the highest genetic distance was found in between parent L. squarrosulus and hybrid pfls1q which means they are genetically most distantly related. Among the hybrid pfls1k and pfls1p showed maximum genetic distance while the most closely related hybrids were pfls1i and pfls1l. The parent L. squarrosulus was most genetically distant individual than the other parent P. florida.

Keywords


Hybrid Mushroom, Genetic Diversity, Polymorphism, RAPD.