Journal of Environment and Sociobiology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Standardization Of Bioethanol Production From The Well-known Weed, Water Hyacinth


Affiliations
1 Department of Biotechnology, Brainware University, Barasat, Kolkata–700125,, India
     

   Subscribe/Renew Journal


Water hyacinth (Eichhornia crassipes) is a very common weed that degrades inland water bodies and responsible for drastic water pollution. This weed possesses about 80% of cellulose within it which is a polysaccharide of the monomeric β-D glucose. Glucose is a good substrate for the production of bioethanol. This process can be accelerated using some microbial strains which are well documented. The microbes that can grow on the water extract of water hyacinth are believed to utilize cellulose as the organic carbon source. These microbes were isolated from the water hyacinth extract and plated on CMC agar and PDA media to get the pure culture. The bacterial colonies were found on CMC media resembled the Actinomycetaceae family and were used as the inoculating culture of the bioethanol production. The small scale pilot fermentation process, without the help of any bioreactor, was carried out using the water hyacinth as a raw material for one month. The presence of ethanol was confirmed using Iodoform test. The process was replicated to confirm the reliability of the results. This bioethanol production process may prove to be cheaper and eco-friendly.

Keywords

Water hyacinth, Pollutant, BOD, Actinomycetaceae, Fermentation, Bioethanol, Green energy, Pollution control, Iodoform test.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Basak, M. K., Bhaduri, S. K., Banik, S., Kundu, S. K. and Sardar, D. 1998. Some aspects of biochemical changes associated with retting of green jute plants. In: Proceedings of International Seminar on Jute and Allied Fibres/Changing Global Scenario. Calcutta, NIRJAFT Publication, pp. 105-108.

  • Bayrakci, A. G., and Koçar, G. 2014. Second-generation bioethanol production from water hyacinth and duckweed in Izmir: a case study. Renew. Sust. Energy Rev., 30: 306-316.

  • Bergier, I., Salis, S. M., Miranda, C. H. B., Ortega, E. and Luengo, C. A. 2012. Biofuel production from water hyacinth in the Pantanal wetland. Ecohydrol. Hydrobiol., 12(1): 77-84.

  • Buller, L. S., Ortega, E., Bergier, I., Mesa-Pérez, J. M., Salis, S. M. and Luengo, C. A. 2015. Sustainability assessment of water hyacinth fast pyrolysis in the Upper Paraguay river basin, Brazil. Sci. Total Environ., 532(1): 281-291.

  • Cheng, J., Lin, R., Song, W., Xia, A., Zhou, J. and Cen, K. 2015. Enhancement of fermentative hydrogen production from hydrolyzed water hyacinth with activated carbon detoxification and bacteria domestication. Int. J. Hydrog. Energy, 40(6): 2545-2551.

  • Cheng, Y-S, Chen, K-Y and Chou, T-H. 2015. Concurrent calcium peroxide pretreatment and wet storage of water hyacinth for fermentable sugar production. Bioresour. Technol., 176: 267-272.

  • Craft, C., Megonigal, P., Broome, S., Stevenson, J., Freese, R., Cornell, J., Lei, Z. and Sacco, J. 2003. The pace of ecosystem development of constructed Spartina alterniflora marshes. Ecol. Appl., 13(5): 1417-1432.

  • Das, P., Solanki, R. and Khanna, M. 2014. Isolation and screening of cellulolytic actinomycetes from diverse habitats. Int. J. Adv. Biotechnol. Res., 5(3),: 438-451.

  • Das, S. P., Ravindran, R., Ghosh, A., Deka, D., Das, D., Jawed, M., Fontes, C. M. G. A. and Goyal, A. 2013. Efficient pretreatment for bioethanol production from water hyacinth (Eichhornia crassipes) involving naturally isolated and recombinant enzymes and its recovery. / Environ. Prog. Sustain. Energy, 33(4): 1396-1404.

  • Ganguly, A., Chatterjee, P. K. and Dey, A. 2012. Studies on ethanol production from water hyacinth—a review. Renew. Sustain. Energy Rev., 16(1): 966-972.

  • Guerrero-Coronilla, I., Morales-Barrera, L. and Cristiani-Urbina, E. 2015. Kinetic, isotherm and thermodynamic studies of amaranth dye biosorption from aqueous solution onto water hyacinth leaves. J. Environ. Manage., 152(1): 99-108.

  • Hu, Z., Ma, X. and Li, L. 2015. Optimal conditions for the catalytic and non-catalytic pyrolysis of water hyacinth. Energy Convers. Management, 94: 337-344.

  • Jiu, B-B., Li, B-X. and Yu, Q-J. 2015. Effects of Pb on pyrolysis behavior of water hyacinth. J. Anal. Appl. Pyrolysis, 112: 270-275.

  • Standardization of bioethanol production from the well-known weed, water hyacinth

  • Lin, R., Cheng, J., Song, W., Ding, L., Xie, B., Zhou, J. and Cen, K. 2015. Characterisation of water hyacinth with microwave-heated alkali pretreatment for enhanced enzymatic digestibility and hydrogen/methane fermentation. Bioresour. Technol., 182: 1-7.

  • Malik, A. 2007. Environmental challenge vis a vis opportunity: the case of water hyacinth. Environ. Int., 33(1): 122-138.

  • Masto, R. E., Kumar, S., Rout, T. K., Sarkar, P., George, J. and Ram, L. C. 2013. Biochar from water hyacinth (Eichhornia crassipes) and its impact on soil biological activity. Catena, 111: 64-71.

  • Merino-Pérez, O., Martínez-Palou, R., Labidi, J., and Luque, R. 2015. Microwaveassisted pretreatment of lignocellulosic biomass to produce biofuels and valueadded products. In: Z. Fang, R. Smith Jr. and X, Qi (eds.), Production of Biofuels and Chemicals with Microwave-Biofuels and Biorefineries, Vol. 3: 197- 224. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9612-5_10

  • Patil, J. H., Antony Raj, M. A. L., Shankar, B. B., Shetty, M. K. and Kuar, B. P. P. 2014. Anaerobic co-digestion of water hyacinth and sheep waste. Energy Procedia, 52: 572-578.

  • Rezania, S., Ponraj, M., Din, M. F. M., Songip, A. R., Sairan, F. M. and Chelliapan, S. 2015. The diverse applications of water hyacinth with main focus on sustainable energy and production for new era: an overview. Renew. Sustain. Energy Rev., 41: 943-954.

  • Robl, D., Mergel, C. M., Costa, P. D. S., Pradella, J. G. D. C. and Padilla, G. 2019. Endophytic actinomycetes as potential producers of hemicellulases and related enzymes for plant biomass degradation. Braz. Arch. Biol. Technol., 62: e19180337.

  • Saini, A., Aggarwal, N. K., Sharma, A. and Yadav, A. 2015. Actinomycetes: a source of lignocellulolytic enzymes. Enzyme Res., 2015: 279381, 15 pp. https://dx.doi. org/10.1155/2015/279381

  • Seelye, R. N. and Turney, T. A. 1959. The iodoform reaction. J. Chem. Educ., 36(11): 572.

  • Valentine, J., Clifton-Brown, J., Hastings, A., Robson, P., Allison, G. and Smith, P. 2012. Food vs. fuel: the use of land for lignocellulosic ‘next generation’energy crops that minimize competition with primary food production. GCB Bioenergy, 4(1): 1-19.

  • Xia, A., Cheng, J., Song, W., Yu, C., Zhou, J. and Cen, K. 2013. Enhancing enzymatic saccharification of water hyacinth through microwave heating with dilute acid pretreatment for biomass energy utilization. Energy, 61: 158-166.

  • Zhang, F., Wang, X., Yin, D., Peng, B., Tan, C., Liu, Y., Tan, X. and Wu, S. 2015. Efficiency and mechanisms of Cd removal from aqueous solution by biochar derived from water hyacinth (Eichhornia crassipes). J. Environ. Manage., 153: 68-73.

  • Zhao, J. and Xia, L. 2010. Ethanol production from corn stover hemicellulosic hydrolysate using immobilized recombinant yeast cells. Biochem. Eng. J., 49(1): 28-32.


Abstract Views: 200

PDF Views: 0




  • Standardization Of Bioethanol Production From The Well-known Weed, Water Hyacinth

Abstract Views: 200  |  PDF Views: 0

Authors

Sayantan Ghosh
Department of Biotechnology, Brainware University, Barasat, Kolkata–700125,, India

Abstract


Water hyacinth (Eichhornia crassipes) is a very common weed that degrades inland water bodies and responsible for drastic water pollution. This weed possesses about 80% of cellulose within it which is a polysaccharide of the monomeric β-D glucose. Glucose is a good substrate for the production of bioethanol. This process can be accelerated using some microbial strains which are well documented. The microbes that can grow on the water extract of water hyacinth are believed to utilize cellulose as the organic carbon source. These microbes were isolated from the water hyacinth extract and plated on CMC agar and PDA media to get the pure culture. The bacterial colonies were found on CMC media resembled the Actinomycetaceae family and were used as the inoculating culture of the bioethanol production. The small scale pilot fermentation process, without the help of any bioreactor, was carried out using the water hyacinth as a raw material for one month. The presence of ethanol was confirmed using Iodoform test. The process was replicated to confirm the reliability of the results. This bioethanol production process may prove to be cheaper and eco-friendly.

Keywords


Water hyacinth, Pollutant, BOD, Actinomycetaceae, Fermentation, Bioethanol, Green energy, Pollution control, Iodoform test.

References