Journal of Environment and Sociobiology

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Isolation of Saline Tolerant Pgpr (ST-PGPR) From Sinking Island of Ghoramara


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1 Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata-70016., India
     

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Soil salinity, a global issue, is a threat to the agricultural crops. The deposition of excess salt in the soil reduces the growth, development and yield of crops. Initially techniques like leaching and flushing were applied to reclaim such salt infested soil. However, the success rate was low and it greatly affected the agroecosystem. This had led to the search for saline tolerant and salt resistant crops. The sinking island of Ghoramara is also facing the problem of soil salinity. In this investigation, soil samples were collected from coastal, transition and agricultural region of the island and the physico-chemical parameters of the soil sample were analyzed to understand its quality. The parameters pH and EC were carefully analyzed since they are of utmost importance for elucidating soil salinity. Saline tolerant bacteria were isolated using increasing concentrations of salt in the growth medium. The isolated bacteria were grown on chrome agar followed by gram staining to identify the genus of the isolated bacteria. This was followed by the study of NPK fixation and solubilization analysis, zinc solubilizing efficiency and siderophore production i.e., iron chelating ability of the isolated strains. Based on the results obtained from the above set of experiments five bacteria were selected. These five bacteria would be identified using 16S rRNA. Further experiments for analyzing plant growth promoting hormone production and other plant growth promoting traits will be carried out with these five bacterial strains in the future. Depending on the result a consortium can be developed, specifically designed for the saline infested soil of Ghoramara to improve their crop productivity. Key words:

Keywords

Salt Tolerance, PGPR, Physico-Chemical Parameter, Soil Microflora.
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  • Abo-Koura, H. A., Bishara, M. M. and Saad, M. M. 2019. Isolation and identification of N2 - fixing, phosphate and potassium solubilizing rhizobacteria and their effect on root colonization of wheat Plant. Int. J. Microbiol. Res., 10(2): 62-76.

  • Albdaiwi, R. N., Khyami-Horani, H., Ayad, J. Y., Alananbeh, K. M. and Al-Sayaydeh, R. 2019. Isolation and characterization of halotolerant plant growth promoting rhizobacteria from Durum wheat (Triticum turgidum subsp. durum)cultivated in saline areas of the dead sea region. Front. Microbiol., 10: 1639. DOI: 10.3389/ fmicb.2019.01639.

  • Ali, U., Shar, T., Ahmad, R., Khatoon, M., Khaskheli, M. A., Laghari, A. H. and Leghari, A. J. 2021. Salinity stress–a threat to rice production breeding strategies to develop salinity tolerance in plants. Meh. J. Sci. Tech., 1(1): 13-17.

  • Babu, S. V., Gopal, A. V., Trimurtulu, N., Babu, G. K. and Lakshmi, B. S. 2020. Isolation, screening and characterization of zinc solubilizing microorganisms from direct sown Paddy (Oryza sativaL.) rhizospheric soils of Andhra Pradesh. Int. J. Curr. Microbiol. App. Sci., 9(9), 3334-3346.

  • Black, C. A. 1965. Methods of Soil Analysis. Part 2 Inc., Publisher; Madison, Wisconsin, USA, 802 pp.

  • Dandwate, S. C. 2020. Analysis of soil samples for its physicochemical parameters from Sangamner city. GSC Biol. Pharm. Sci., 12(2): 123-128.

  • Egamberdieva, D., Wirth, S., Bellingrath-Kimura, S. D., Mishra, J. and Arora, N. K. 2019. Salt-tolerant plant growth promoting rhizobacteria for enhancing crop productivity of saline soils. Front. Microbiol., 10: 2791. DOI: 10.3389/ fmicb.2019.02791.

  • Goswami, D., Dhandhukia, P., Patel, P. and Thakker, J. N. 2014. Screening of PGPR from saline desert of Kutch: growth promotion in Arachis hypogea by Bacillus licheniformisA2 ? Microbiol. Res., 169(1): 66-75.

  • Grattan, S. R., Zeng, L., Shannon, M. C. and Roberts, S. R. 2002. Rice is more sensitive to salinity than previously thought. Calif. Agric., 56(6): 189-195.

  • Hoang, T. M. L., Moghaddam, L., Williams, B., Khanna, H., Dale, J. and Mundree, S. G. 2015. Development of salinity tolerance in rice by constitutive-overexpression of genes involved in the regulation of programmed cell death. Front. Plant Sci., 6: 175. DOI: 10.3389/flps.2015.00175

  • Karpagam, T. and Nagalakshmi, P. K. 2014. Isolation and characterization of phosphate solubilizing microbes from agricultural soil. Int. J. Curr. Microbiol. App. Sci., 3(3): 601-614.

  • Isolation of Saline tolerant PGPR (ST-PGPR) from sinking island of Ghoramara 135

  • Pérez-Miranda, S., Cabirol, N., George-Téllez, R., Zamudio-Rivera, L. S. and Fernández, F. J. 2007. O-CAS, a fast and universal method for siderophore detection. J. Microbiol. Methods, 70(1): 127-131.

  • Rashmi, Y. C., Reshmi, R., Poornima, R. and Kumar, S. 2017. Isolation and characterization of microorganisms from agriculture soil of Magnifera indica Orchard. Int. J. Curr. Microbiol. App. Sci., 6(6): 2707-2713.

  • Saxena, S., Kumar, R., Tomar, A., Singh, J., Purushottam and Dhyani, B. P. 2020. Isolation, biochemical characterization and potassium solubilization efficiency of different microbial isolates. Int. J. Curr. Microbiol. App. Sci., 9(6): 2667-2680.

  • Shomi, F. Y., Uddin, M. B. and Zerin, T. 2021. Isolation and characterization of nitrogen-fixing bacteria from soil sample in Dhaka, Bangladesh. Stamford J. Microbiol., 11(1): 11-13.

  • Shultana, R., Kee Zuan, A. T., Yusop, M. R. and Saud, H. M. 2020. Characterization of salt-tolerant plant growth-promoting rhizobacteria and the effect on growth and yield of saline-affected rice. PLoS One, 15(9): e0238537. DOI: 10.1371/ journal.pone.0238537


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  • Isolation of Saline Tolerant Pgpr (ST-PGPR) From Sinking Island of Ghoramara

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Authors

Sayan Kamila
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata-70016., India
Soumya Mondal
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata-70016., India
Bidisha Chatterjee
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata-70016., India
Bedaprana Roy
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata-70016., India
Arup Kumar Mitra
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata-70016., India

Abstract


Soil salinity, a global issue, is a threat to the agricultural crops. The deposition of excess salt in the soil reduces the growth, development and yield of crops. Initially techniques like leaching and flushing were applied to reclaim such salt infested soil. However, the success rate was low and it greatly affected the agroecosystem. This had led to the search for saline tolerant and salt resistant crops. The sinking island of Ghoramara is also facing the problem of soil salinity. In this investigation, soil samples were collected from coastal, transition and agricultural region of the island and the physico-chemical parameters of the soil sample were analyzed to understand its quality. The parameters pH and EC were carefully analyzed since they are of utmost importance for elucidating soil salinity. Saline tolerant bacteria were isolated using increasing concentrations of salt in the growth medium. The isolated bacteria were grown on chrome agar followed by gram staining to identify the genus of the isolated bacteria. This was followed by the study of NPK fixation and solubilization analysis, zinc solubilizing efficiency and siderophore production i.e., iron chelating ability of the isolated strains. Based on the results obtained from the above set of experiments five bacteria were selected. These five bacteria would be identified using 16S rRNA. Further experiments for analyzing plant growth promoting hormone production and other plant growth promoting traits will be carried out with these five bacterial strains in the future. Depending on the result a consortium can be developed, specifically designed for the saline infested soil of Ghoramara to improve their crop productivity. Key words:

Keywords


Salt Tolerance, PGPR, Physico-Chemical Parameter, Soil Microflora.

References