Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Isolation and Characterization of Prospective Salt Tolerant Bacteria With Plant Growth Promoting Properties From Mangroves of Sundarban, West Bengal, India


Affiliations
1 Post-Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata-700016., India
2 Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata-700016., India
3 Vivekananda Institute of Biotechnology, South 24 Parganas, Pin-743 338, West Bengal, India., India
     

   Subscribe/Renew Journal


Increasing soil salinity acts as a major abiotic stress for crop plants. Increasing global temperatures are leading to greater evaporation from soil, along with change in rainfall patterns, which is resulting in reduced soil water availability for crop plants and increased soil salinity. Consequently, crop plants face water and nutrient shortage leading to yield losses. In fact, crop plants cannot be grown easily on such saline soil without some form of remediation. Plant Growth Promoting Rhizobacteria (PGPR) have shown prospective results in this regard. Halotolerant PGPRs have the ability to grow in such saline soils, while providing plant roots in the vicinity with growth nutrients and hormones. In the present study, we obtained six bacterial isolates from mangrove pneumatophores of Aegialitis rotundifoliaRoxb. and Ceriops tagal C. B. Rob. with associated rhizobial soil from Kshetra Mohanpur site in the Sundarbans of West Bengal. They were screened for salt tolerance, nitrogen fixation, phosphate solubilizing, potassium solubilizing and auxin synthesizing ability. Two of the six isolates showed all these properties. Hence, we propose their use as halotolerant PGPR biofertilizers for soil bioremediation.

Keywords

Plant Growth Promoting Rhizobacteria, Biofertilizer, Soil Salinity, Climate Change.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Ahmad, M., Nadeem, S. M., Naveed, M. and Zahir, Z. A. 2016. Potassium-solubilizing bacteria and their application in agriculture. In: V. Meena, B. Maurya, J. Verma and R. Meena (eds.), Potassium Solubilizing Microorganisms for Sustainable Agriculture. Springer, New Delhi.
  • Alippi, A. M. 2019. Data associated with the characterization and presumptive identification of Bacillus and related species isolated from honey samples by using HiCrome Bacillus agar. Data. Br., 25: 104206. DOI: 10.1016/j. dib.2019.104206.
  • Ullah, A., Bano, A. and Khan, N. 2021. Climate change and salinity effects on crops and chemical communication between plants and plant growth-promoting microorganisms under stress. Front. Sustain. Food Syst., 5: 618092. DOI: 10.3389/fsufs.2021.618092
  • Bal, H. B., Nayak, L., Das, S. and Adhya, T. K. 2013. Isolation of ACC deaminase producing PGPR from rice rhizosphere and evaluating their plant growth promoting activity under salt stress. Plant Soil, 366: 93-105.
  • Bhutani, N., Maheshwari, R., Negi, M. and Suneja, P. 2018. Optimization of IAA production by endophytic Bacillusspp. from Vigna radiatafor their potential use as plant growth promoters. Isr. J. Plant Sci., 65(1-2): 83-96.
  • Breedt, G., Labuschagne, N. and Coutinho, T. A. 2017. Seed treatment with selected plant growth-promoting rhizobacteria increases maize yield in the field. Ann. Appl. Biol., 171: 229-236.
  • Cassán, F., Perrig, D., Sgroy, V., Masciarelli, O., Penna, C., and Luna, V. 2009. Azospirillum brasilense Az39 and Bradyrhizobium japonicum E109, inoculated singly or in combination, promote seed germination and early seedling growth in corn (Zea mays L.) and soybean (Glycine max L.). Eur. J. Soil Biol. 45: 28-35.
  • Cordero, I., Balaguer, L., Rincón, A. and Pueyo, J. J. 2018. Inoculation of tomato plants with selected PGPR represents a feasible alternative to chemical fertilization under salt stress. J. Plant Nutr. Soil Sci., 181: 694-703.
  • Dey, M., Chatterjee, S., Dhara, B., Roy, I. and Mitra, A. K. 2022. Promoting crop growth with symbiotic microbes in agro-ecosystems—I. In: Microbes and Microbial Biotechnology for Green Remediation, pp. 117-133. Elsevier.
  • Eswar, D., Karuppusamy, R. and Chellamuthu, S. 2021. Drivers of soil salinity and their correlation with climate change. Curr. Opin. Environ. Sustain., 50: 310-318.
  • Kaymak, H. C. 2010. Potential of PGPR in agricultural innovations. In: D. Maheshwari (ed.), Plant Growth and Health Promoting Bacteria. Microbiology Monographs, Vol. 18. Springer-Verlag Berlin, Heidelberg.
  • Kundu, A., Paul, S., Biswas, S. J., Halder, P., Prasad, R. K., Dey, R., Dhara, B. and Mitra, A. K. 2022. Nature to lab transfer story of the nutritious alga Catenella: a comprehensive review. Vegetos. DOI: 10.1007/s42535-022-00475-3
  • Ghimire, K. M. and Vikas, M. 2012. Climate change – impact on the Sundarbans, a case study. Int. J. Environ. Sci., 2: 7-15.
  • Muthukumarasamy, R., Revathi, G., Vadivelu, M., and Arun, K. 2017. Isolation of bacterial strains possessing nitrogen-fixation, phosphate and potassium-solubilization and their inoculation effects on sugarcane. Indian J. Exp. Biol., 55: 161-170.
  • Pramanik, A., Sengupta, S. and Bhattacharyya, M. 2019 Microbial diversity and community analysis of the Sundarbans Mangrove, a World Heritage Site. Chapter 5. In: S. Das and R. Dash (eds.), Microbial Diversity in the Genomic Era. Academic Press, pp. 65-76.
  • Rasool, S., Hameed, A., Azooz, M. M., Rehman, M. U., Siddiqi, T. O. and Ahmad, P. 2013. Salt Stress: causes, types and responses of plants. In: P. Ahmad, M. Azooz and M. Prasad (eds.), Ecophysiology and Responses of Plants under Salt Stress. DOI: 10.1007/978-1-4614-4747-4_1. Springer, New York, NY.
  • Sahoo, K. and Dhal, N. K. 2009. Potential microbial diversity in mangrove ecosystems: a review. Indian J. mar. Sci., 38: 249-256.
  • Kang, S-M., Radhakrishnan, R., Khan, A. L., Kim, M-J., Park, J-M., Kim, B-R., Shin, D-H. and Lee, I-J. 2014. Gibberellin secreting rhizobacterium, Pseudomonas putidaH-2-3 modulates the hormonal and stress physiology of soybean to improve the plant growth under saline and drought conditions. Plant Physiology and Biochemistry, 84: 115-124.
  • Sarker, A. and Rashid, J. 2013. Technical Report of Quantification of IAA by Microbes, pp. 1-2.
  • Schneegurt, M. 2012. Media and conditions for the growth of halophilic and halotolerant bacteria and archaea. In: R. H. Vreeland (ed.), Advances in Understanding the Biology of Halophilic Microoganisms. Springer, Dordrecht, pp. 35-58.DOI: 10.1007/978-94-007-5539-0_2.
  • Sengupta, A, and Chaudhuri S. 1991. Ecology of heterotrophic dinitrogen fixation in the rhizosphere of mangrove plant community at the Ganges river estuary in India. Oecologia, 87(4): 560-564.
  • Sharma, A., Dev, K., Sourirajan, A. and Choudhari, M. 2021. Isolation and characterization of salt-tolerant bacteria with plant growth-promoting activities from saline agricultural fields of Haryana, India. J. Genet. Eng. Biotechnol., 19: 99. DOI: 10.1186/s43141-021-00186-3.
  • Vafadar, F., Amooaghaie, R., and Otroshy, M. 2014. Effects of plant-growth-promoting rhizobacteria and arbuscular mycorrhizal fungus on plant growth, stevioside, NPK, and chlorophyll content of Stevia rebaudiana, J. Plant Interact., 9(1): 128-136. Zablackis, E. and Santos, G. A. 1986. The carrageenan of Catenella nipae Zanard., a marine red alga. Bot. Mar., 29: 319-322.
  • Zhang, Y., Yang, Q., Ling, J., Van Nostrand, J. D., Shi, Z., Zhou, J. and Dong, J. 2017. Diversity and structure of diazotrophic communities in mangrove rhizosphere, revealed by high-throughput sequencing. Front. Microbiol., 8: 2032. DOI: 10.3389 fmicb.2017.02032.

Abstract Views: 286

PDF Views: 0




  • Isolation and Characterization of Prospective Salt Tolerant Bacteria With Plant Growth Promoting Properties From Mangroves of Sundarban, West Bengal, India

Abstract Views: 286  |  PDF Views: 0

Authors

Shivashis Bikram Banerjee
Post-Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata-700016., India
Debapriya Maitra
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata-700016., India
Bedaprana Roy
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata-700016., India
Bikram Dhara
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata-700016., India
Ramalakshmi Datta
Vivekananda Institute of Biotechnology, South 24 Parganas, Pin-743 338, West Bengal, India., India
Sanjay Haldar
Vivekananda Institute of Biotechnology, South 24 Parganas, Pin-743 338, West Bengal, India., India
Arup Kumar Mitra
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata-700016., India

Abstract


Increasing soil salinity acts as a major abiotic stress for crop plants. Increasing global temperatures are leading to greater evaporation from soil, along with change in rainfall patterns, which is resulting in reduced soil water availability for crop plants and increased soil salinity. Consequently, crop plants face water and nutrient shortage leading to yield losses. In fact, crop plants cannot be grown easily on such saline soil without some form of remediation. Plant Growth Promoting Rhizobacteria (PGPR) have shown prospective results in this regard. Halotolerant PGPRs have the ability to grow in such saline soils, while providing plant roots in the vicinity with growth nutrients and hormones. In the present study, we obtained six bacterial isolates from mangrove pneumatophores of Aegialitis rotundifoliaRoxb. and Ceriops tagal C. B. Rob. with associated rhizobial soil from Kshetra Mohanpur site in the Sundarbans of West Bengal. They were screened for salt tolerance, nitrogen fixation, phosphate solubilizing, potassium solubilizing and auxin synthesizing ability. Two of the six isolates showed all these properties. Hence, we propose their use as halotolerant PGPR biofertilizers for soil bioremediation.

Keywords


Plant Growth Promoting Rhizobacteria, Biofertilizer, Soil Salinity, Climate Change.

References