Journal of Environment and Sociobiology

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Development of Species Specific DNA Marker as Barcode Sequence of Greater Indian Rhinoceros [Rhinoceros unicornis] from Northeast India


Affiliations
1 Department of Biotechnology, Assam University, Silchar-788011, Assam, India
2 Department of Vetarinary Physiology, WBUAFS, Kolkata-700 037, W.B., India
     

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Northeast India is a reservoir of rich biodiversity, especially Greater one-horned rhinoceros (Rhinoceros unicornis) which is facing extinction. DNA barcoding being an exciting tool for accurate documention of biodiversity with a gene sequence, in the present article DNA barcode of R. unicornis is determined for the first time by amplifying mitochondrial COI gene region using cross species primer designing and other bioinformatics analysis (Accession No. JN41700). Phylogeny analysis of obtained sequence with other related species distinctly maintains the assigned taxonomic position.

Keywords

DNA Barcoding, Mitochondrial DNA, COI-5', Rhinoceros Unicornis
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  • Development of Species Specific DNA Marker as Barcode Sequence of Greater Indian Rhinoceros [Rhinoceros unicornis] from Northeast India

Abstract Views: 390  |  PDF Views: 3

Authors

Sankar Kumar Ghosh
Department of Biotechnology, Assam University, Silchar-788011, Assam, India
Prabal Ranjan Ghosh
Department of Vetarinary Physiology, WBUAFS, Kolkata-700 037, W.B., India
Tarikul Huda Mazumder
Department of Biotechnology, Assam University, Silchar-788011, Assam, India
Miranda Kshetrimayum
Department of Biotechnology, Assam University, Silchar-788011, Assam, India

Abstract


Northeast India is a reservoir of rich biodiversity, especially Greater one-horned rhinoceros (Rhinoceros unicornis) which is facing extinction. DNA barcoding being an exciting tool for accurate documention of biodiversity with a gene sequence, in the present article DNA barcode of R. unicornis is determined for the first time by amplifying mitochondrial COI gene region using cross species primer designing and other bioinformatics analysis (Accession No. JN41700). Phylogeny analysis of obtained sequence with other related species distinctly maintains the assigned taxonomic position.

Keywords


DNA Barcoding, Mitochondrial DNA, COI-5', Rhinoceros Unicornis