Isolation and Estimation of Clerodendrin a in Clerodendrum Phlomidis and Premna Integrifolia Root

Clerodendrin A, a chemical marker was isolated from the ischolar_main of Clerodendrum phlomidis (Verbanaceae). We here report first time, the presence of clerodendrinAin another Verbanaceae drug, Premna integrifolia. Further, HPTLC method was developed to quantify isolated clerodendrinAin C. phlomidis and P. integrifolia ischolar_mains using n-hexane: ethyl formate (7:3) as mobile phase, pre-coated TLC plates (silica gel 60 F₂₅₄) as stationary phase and H₂SO₄ as derivatizing agent. The method was validated in terms of linearity, precision, repeatability and accuracy. The limit of detection and limit of quantification was determined on the basis of signal to noise ratio. Content of clerodendrin A was found to be 0.073 and 0.04% w/w in C. phlomidis and P. integrifolia respectively. The developed HPTLC method was easy, simple, and precise.