Journal of Pharmaceutical Research

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Development Of a Simple HPLC Method for the Quantitation of Artemisinin in Artemisia Annua Herb


Affiliations
1 Research and Development Division, Central Indian Pharmacopoeia Laboratory, Indian Pharmacopoeia Commission, Govt. of India, Ministry of Health & FamilyWelfare, Sector-23, Rajnagar, Ghaziabad-201002, India
2 Department of Chemistry, M. M. H. College, Ghaziabad (U.P.), India
 

A high-performance liquid chromatographic (HPLC) method has been developed for the determination of Artemisinin in Artemisia annua Herb. The HPLC separation was carried out in isocratic mode using C18, sunfire column (4.6 × 250 mm, 5 μm particle size) with a mobile phase composed of acetonitrile : water (85:15 v/v) at a flow rate of 1.0 ml/min. The detection was monitored at 216 nm. The method validation parameters showed good results for linearity, precision, accuracy, specificity, and in recovery studies. The calibration curve for Artemisinin was found linear from the range of 500 to 1200 μg/ml. The interday and intraday studies (relative standard deviation) was obtained within the limits. The proposed HPLC method is precise, accurate and rapid for determination of Artemisinin in Artemisia annua Herb.

Keywords

Artemisinin, HPLC, Artemisia Annua Herb.
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  • Development Of a Simple HPLC Method for the Quantitation of Artemisinin in Artemisia Annua Herb

Abstract Views: 185  |  PDF Views: 99

Authors

P. K. Saini
Research and Development Division, Central Indian Pharmacopoeia Laboratory, Indian Pharmacopoeia Commission, Govt. of India, Ministry of Health & FamilyWelfare, Sector-23, Rajnagar, Ghaziabad-201002, India
C. L. Jain
Department of Chemistry, M. M. H. College, Ghaziabad (U.P.), India
R. M. Singh
Research and Development Division, Central Indian Pharmacopoeia Laboratory, Indian Pharmacopoeia Commission, Govt. of India, Ministry of Health & FamilyWelfare, Sector-23, Rajnagar, Ghaziabad-201002, India
S. C. Mathur
Research and Development Division, Central Indian Pharmacopoeia Laboratory, Indian Pharmacopoeia Commission, Govt. of India, Ministry of Health & FamilyWelfare, Sector-23, Rajnagar, Ghaziabad-201002, India
G. N. Singh
Research and Development Division, Central Indian Pharmacopoeia Laboratory, Indian Pharmacopoeia Commission, Govt. of India, Ministry of Health & FamilyWelfare, Sector-23, Rajnagar, Ghaziabad-201002, India

Abstract


A high-performance liquid chromatographic (HPLC) method has been developed for the determination of Artemisinin in Artemisia annua Herb. The HPLC separation was carried out in isocratic mode using C18, sunfire column (4.6 × 250 mm, 5 μm particle size) with a mobile phase composed of acetonitrile : water (85:15 v/v) at a flow rate of 1.0 ml/min. The detection was monitored at 216 nm. The method validation parameters showed good results for linearity, precision, accuracy, specificity, and in recovery studies. The calibration curve for Artemisinin was found linear from the range of 500 to 1200 μg/ml. The interday and intraday studies (relative standard deviation) was obtained within the limits. The proposed HPLC method is precise, accurate and rapid for determination of Artemisinin in Artemisia annua Herb.

Keywords


Artemisinin, HPLC, Artemisia Annua Herb.