Open Access Open Access  Restricted Access Subscription Access

Phytochemical Analysis and Free-Radical Scavenging Activity of Flacourtia indica (Burm.f.) Merr.


Affiliations
1 Central Indian Pharmacopoeia Laboratory, Govt. of India, Ministry of Health and Family Welfare, Sector -23, Raj Nagar, Ghaziabad, U.P.- 201002, India
2 I.T.S. Paramedical College (Pharmacy), Delhi-Meerut Road, Murad Nagar, Ghaziabad, U.P.- 201206, India
3 Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hamdard University, New Delhi - 110 062, India
 

Flacourtia indica dichloromethane (DCM), butanol (BuOH), methanol (MeOH) extract of stem bark and methanolic extract of leaves were screened for in vitro antioxidant activity using different models. Subsequent quantification showed the presence of 15.62 and 11.53%w/w phenolics; 1.15 and 1.80%w/ w of flavonol in methanol extract of stem bark and leaf of Flacourtia indica (F. indica) respectively. The methanolic extract showed an effective DPPH radical scavenging activity with low IC50 values of 17.5 and 21 µg/ml respectively and hydroxyl radical scavenging by p-NDA method with IC50 value of 350.23 µg/ml. Butanolic extract showed nitric oxide radical inhibition activity with IC50 value of 28.5 µg/ml. The greater amount of phenolic compounds leads to more potent radical scavenging effect as shown by F. indica extracts. The antioxidant property of the extracts may be due to presence of phenolic content.

Keywords

Flacourtia indica, DPPH, Nitric Oxide Radical Inhibition Assay, Scavenging of Hydroxyl Radical by p-NDA.
User
Notifications
Font Size


Abstract Views: 193

PDF Views: 96




  • Phytochemical Analysis and Free-Radical Scavenging Activity of Flacourtia indica (Burm.f.) Merr.

Abstract Views: 193  |  PDF Views: 96

Authors

Madan Swati
Central Indian Pharmacopoeia Laboratory, Govt. of India, Ministry of Health and Family Welfare, Sector -23, Raj Nagar, Ghaziabad, U.P.- 201002, India
Singh Gyanendra Nath
Central Indian Pharmacopoeia Laboratory, Govt. of India, Ministry of Health and Family Welfare, Sector -23, Raj Nagar, Ghaziabad, U.P.- 201002, India
Kumar Yatendra
I.T.S. Paramedical College (Pharmacy), Delhi-Meerut Road, Murad Nagar, Ghaziabad, U.P.- 201206, India
Kohli Kanchan
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hamdard University, New Delhi - 110 062, India
Singh Raman Mohan
Central Indian Pharmacopoeia Laboratory, Govt. of India, Ministry of Health and Family Welfare, Sector -23, Raj Nagar, Ghaziabad, U.P.- 201002, India
Om Prakash
Central Indian Pharmacopoeia Laboratory, Govt. of India, Ministry of Health and Family Welfare, Sector -23, Raj Nagar, Ghaziabad, U.P.- 201002, India

Abstract


Flacourtia indica dichloromethane (DCM), butanol (BuOH), methanol (MeOH) extract of stem bark and methanolic extract of leaves were screened for in vitro antioxidant activity using different models. Subsequent quantification showed the presence of 15.62 and 11.53%w/w phenolics; 1.15 and 1.80%w/ w of flavonol in methanol extract of stem bark and leaf of Flacourtia indica (F. indica) respectively. The methanolic extract showed an effective DPPH radical scavenging activity with low IC50 values of 17.5 and 21 µg/ml respectively and hydroxyl radical scavenging by p-NDA method with IC50 value of 350.23 µg/ml. Butanolic extract showed nitric oxide radical inhibition activity with IC50 value of 28.5 µg/ml. The greater amount of phenolic compounds leads to more potent radical scavenging effect as shown by F. indica extracts. The antioxidant property of the extracts may be due to presence of phenolic content.

Keywords


Flacourtia indica, DPPH, Nitric Oxide Radical Inhibition Assay, Scavenging of Hydroxyl Radical by p-NDA.