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A method for simultaneous estimation of Amlodipine besylate and Hydrochlorothiazide in tablet dosage form has been described. The method is based on UV-Spectrophotometric determination of two drugs, using simultaneous equation method. It involves absorbance measurement at 236.17 nm (emax of Amlodipine besylate) and 268.58 nm (emax of Hydrochlorothiazide) in methanol. Linearity was obtained in the range of 6-33 µg/ml for Amlodipine besylate and 3-33 μg/ml for Hydrochlorothiazide. The method allows rapid analysis of binary pharmaceutical formulation with accuracy. Limit of Quantification and Limit of Detection for Amlodipine besylate at 236.17 nm was found to be 2.1 and 0.6, respectively and for Hydrochlorothiazide 0.9 and 0.28, respectively. Limit of Quantification and Limit of Detection for Amlodipine besylate at 268.58 nm was found to be 1.0 and 0.31, respectively and for Hydrochlorothiazide 0.15 and 0.045, respectively. Results of analysis for this method were validated statistically and were found satisfactory.


Amlodipine Besylate (AML), Hydrochlorothiazide (HCT), UV-Spectrophotometry.
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